Volumetric imaging of blood perfusion and tissue morphology in the cochlea

耳蜗血液灌注和组织形态的体积成像

• 批准号: 8300967
• 负责人: Ruikang Wang
• 金额: $ 52.66万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8300967
• 关键词: Affect; Algorithms; Animal Model; Animals; Anti-Inflammatory Agents; Arteries; Beds; Blood Cells; Blood Pressure; Blood Vessels; Blood capillaries; Blood flow; Caliber; Cavia; Clinical; Cochlea; Cochlear duct; Contrast Media; Data; Development; Devices; Diagnosis; Edema; Endolymphatic duct; External auditory canal; Gerbils; Goals; Histopathology; Human; Image; Incidence; Individual; Labyrinth; Laser-Doppler Velocimetry; Magnetic Resonance Imaging; Measurement; Measures; Meniere' s Disease; Metric; Microcirculation; Morphology; Noise; Optical Coherence Tomography; Optics; Organ of Corti; Outpatients; Positioning Attribute; Property; Relative (related person); Research; Resolution; Scanning; Sensorineural Hearing Loss; Speed; Surgical incisions; System; Techniques; Testing; Three-Dimensional Image; Time; Tissues; Translating; Veins; Vestibular membrane; Work; arm; arteriole; base; blood perfusion; capillary; cost; design; disease diagnosis; endolymphatic sac; imaging modality; imaging probe; improved; in vivo; instrument; light scattering; microangiography; miniaturize; novel; operation; optical imaging; particle; prototype; public health relevance; success; tool

DESCRIPTION (provided by applicant): Non-invasive techniques for determining blood flow in the cochlea and imaging its tissue morphology are of paramount importance for the improved understanding, diagnosis, and treatment of sudden sensorineural hearing loss (SSHL) and Meniere's disease. Currently there is no device capable of in vivo measurement of volumetric cochlear blood flow (CoBF) or cochlear tissue morphology. We propose to develop an outpatient imaging instrument that is able to measure CoBF in the human inner ear and categorize the flow level as normal or abnormal. The instrument will also image the position of Reissner's membrane and determine if there is a cochlea hydrops. The instrument can be applied in the outpatient setting and for the first time will provide a metric for determining the basis for, and the form and incidence of 'vascular' SSHL and the rational treatment with vasoactive and anti-inflammatory agents. It will be able to confirm the hydrops form of Meniere's disease diagnosis. The design of the proposed instrument is based on a novel optical imaging modality, 3D optical microangiography (OMAG) and optical coherence tomography (OCT) that we have recently developed. OMAG is able, for the first time, to image the 3D distribution of dynamic blood perfusion, down to the capillary level, within the microcirculation tissue beds at an imaging depth up to 2.00mm into tissue. OMAG produces imaging contrast via endogenous light scattering from moving particles (e.g. flowing blood cells within open vessels), thus no exogenous contrast agents are necessary. It is markedly different from LDF as one may obtain a calibrated metric for the blood flow. Using the OMAG system, we have been able to capture in vivo 3D blood flow images, down to capillary level resolution, from the cochlea in gerbils. The OCT mode of operation of the instrument provided images in the plane passing through the organ of Corti and scala media space that revealed the position of Reissner's membrane. In the proposed research, we aim to design this novel imaging system for in vivo imaging cochlear tissue morphology and CoBF and will test it in an animal model. By its use in humans we expect to define blood flow involvement in SSHL since, with the instrument, blood flow can be systematically investigated for the first time at the resolution level of identifiable vessel classes (artery, arterioles, capillaries, venues and vein). We will correlate the data obtained from use of the instrument with that obtained by high field strength MRI. The project design also includes a clinical arm that aims to translate the technique into the clinical settings. PUBLIC HEALTH RELEVANCE: We propose to develop a novel optical imaging instrument that can provide the simultaneous, quantitative assessment of blood flow and tissue morphology in the cochlea in clinical settings. This novel imaging instrument will become an important tool to improve the understanding, diagnosis and treatment of sudden sensorineural hearing loss and Meniere's disease

描述（由申请人提供）：用于测定耳蜗血流和其组织形态学成像的非侵入性技术对于提高对突发性感音神经性听力损失（SSHL）和梅尼埃病的理解、诊断和治疗至关重要。目前还没有能够在体内测量耳蜗体积血流量（CoBF）或耳蜗组织形态的设备。我们建议开发一种门诊成像仪器，能够测量人内耳的CoBF，并将流量水平分类为正常或异常。该仪器还将成像雷氏膜的位置，并确定是否有耳蜗积液。该仪器可用于门诊，首次为确定“血管性”SSHL的基础、形式和发生率以及合理使用血管活性和抗炎药物治疗提供了一个指标。它将能够确诊梅尼埃氏病的水肿形式。该仪器的设计基于一种新的光学成像方式，即我们最近开发的3D光学微血管造影（OMAG）和光学相干断层扫描（OCT）。OMAG首次能够成像微循环组织床内动态血流灌注的3D分布，直至毛细血管水平，成像深度可达组织内部2.00mm。OMAG通过运动颗粒（如开放血管内流动的血细胞）的内源性光散射产生成像造影剂，因此不需要外源性造影剂。它与LDF明显不同，因为人们可以获得血流的校准度量。使用OMAG系统，我们已经能够从沙鼠耳蜗中捕捉到体内3D血流图像，精确到毛细血管水平的分辨率。该仪器的OCT操作模式提供了通过Corti器官和scala介质空间的平面图像，显示了Reissner膜的位置。在本研究中，我们的目标是设计一种新的成像系统，用于在体内成像耳蜗组织形态和CoBF，并将在动物模型中进行测试。通过在人体中使用该仪器，我们期望确定SSHL中血流的参与情况，因为有了该仪器，可以首次在可识别的血管类别（动脉、小动脉、毛细血管、场所和静脉）的分辨率水平上系统地研究血流。我们将把使用该仪器获得的数据与高场强MRI获得的数据联系起来。该项目设计还包括一个临床臂，旨在将该技术转化为临床环境。