Genetic Studies of the Synapse

突触的遗传学研究

• 批准号: 8277923
• 负责人: LILY Y JAN
• 金额: $ 37.97万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8277923
• 关键词: 1-Phosphatidylinositol 3-Kinase; Action Potentials; Adopted; Ankyrins; Area; Autistic Disorder; Award; Axon; Binding; Binding Proteins; Brain; COX7A2L Protein; Calcium; Calcium Spikes; Cell membrane; Chloride Channels; Convulsions; Coupling; Dendrites; Distal; Endoplasmic Reticulum; Epilepsy; Family; Feedback; Fluorescence; Freedom; Generations; Genes; Genetic; Genetic Translation; Glutamate Receptor; Goals; Golgi Apparatus; Grant; Growth; Hippocampus (Brain); Inherited; Instruction; Ion Channel; KRP protein; Kinesin; Kinetics; Learning; Legal patent; Mammalian Cell; Mediating; Membrane; Membrane Potentials; Mental Retardation; Mental disorders; Messenger RNA; Microtubule Bundle; Microtubule-Associated Proteins; Microtubules; Modeling; Motor; Movement; Mutant Strains Mice; Mutation; Myokymia; N-Methyl-D-Aspartate Receptors; N-Methylaspartate; Neonatal; Neurons; Paper; Phosphotransferases; Physiological; Plants; Plus End of the Microtubule; Positioning Attribute; Potassium; Potassium Channel; Property; Protein Binding; Proteins; Publications; Publishing; Regulation; Reporter; Research Design; Research Peer Review; Risk; Role; Signal Pathway; Signal Transduction; Sirolimus; Site; Structure; Suppressor Mutations; Surface; Synapses; Synaptic Potentials; Synaptic plasticity; TSC1 gene; TSC2 gene; Testing; Translations; Tuberous Sclerosis; Tuberous sclerosis protein complex; Varicosity; Vesicle; Voltage-Gated Potassium Channel; Xenopus oocyte; Yeasts; base; density; dimer; experience; expression cloning; follow-up; genetic regulatory protein; information processing; inhibitor/antagonist; insight; mTOR protein; meetings; mutant; neuronal excitability; novel; response; sensor; synaptic function; synaptic inhibition; trafficking; voltage

The long-term objectives are to study how potasslunn channels regulation contributes to neuronal signaling. The specific aims are to understand how the dendritic voltage-gated potassium channels and G proteinactivated inwardly rectifying potassium (GIRK) channels are regulated in hippocampal neurons, and how these potassium channels contribute to neuronal signaling and synaptic plasticity. The research design involves examination of mutant mice with altered or deleted potassium channel regulatory proteins, for regulation of either the local synthesis of channels in the dendrite or the activity or kinetic properties of the channel on the cell membrane. Because the Kv1 voltage-gated potassium channels reside on both dendrites and axons, the mosaic analysis with double markers (MADM) approach will be employed to study the physiological role of dendritic Kv1 channels.

长期目标是研究钾通道调节如何促进神经元信号传导。 具体目的是了解树突状细胞电压门控性钾通道和G蛋白是如何激活 在海马神经元中，内向整流钾通道（GIRK）受到调节， 这些钾通道有助于神经元信号传导和突触可塑性。研究设计 涉及检查具有改变或缺失的钾通道调节蛋白的突变小鼠， 调节枝晶中通道的局部合成或枝晶的活性或动力学性质 细胞膜上的通道。因为Kv1电压门控钾通道位于两个树突上 和轴突，将采用双标记镶嵌分析（MADM）方法来研究 树突状Kv1通道的生理作用。