Proteomics Core

蛋白质组学核心

• 批准号: 8230234
• 负责人: EROL E GULCICEK
• 金额: $ 21.11万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8230234
• 关键词: Algorithms; Amino Acid Sequence; Amino Acids; Basic Science; Biological Markers; Biological Products; Biomedical Research; Biometry; Biophysics; Biotechnology; Chemistry; Clinical; Clinical Research; Commit; Communities; Consult; Custom; Data; Detection; Disease; Emergency Situation; Emerging Communicable Diseases; Event; Experimental Designs; Fluorescence; Foundations; Goals; High Pressure Liquid Chromatography; Infectious Diseases Research; Institution; Ion-Exchange Chromatography Procedure; Laboratories; Lasers; Mass Spectrum Analysis; Methodology; Modification; Molecular Sieve Chromatography; Molecular Weight; N-terminal; National Institute of Allergy and Infectious Disease; Peptide Sequence Determination; Peptide Synthesis; Peptides; Phosphoproteins; Post-Translational Protein Processing; Proteins; Proteomics; Relative (related person); Research; Research Personnel; Research Support; Resources; Sampling; Services; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Technology; Universities; base; biodefense; clinically relevant; design; gel electrophoresis; improved; instrumentation; light scattering; meetings; member; pre-clinical research; programs; protein profiling; research and development; tool

Regional Center of Excellence (RCE) for Biodefense and Emerging Infectious Disease Research program, the Northeast Biodefense Center (NBC) constitutes a biomedical research program with cores supporting its multifaceted research and development activities. As part of this program, the NBC Proteomics Core will operate within the W.M. Keck Foundation Biotechnology Resource Laboratory at Yale University and will continue to provide a very broad range of bioanalytical services to support basic science, preclinical and clinical research programs in the NBC. In addition, in periods of national biodefense or emerging infectious disease emergency, the NBC Proteomics Core will be prepared and devote all of its considerable resources to meeting the challenge. The NBC Proteomics Core will offer these services from all of its proteomics sections: (1) Mass Spectrometry and Protein Profiling resources that utilize state of the art mass spectrometry instrumentation (LC-FT-ICR, LC-LTQ-Orbitrap, LC-QTRAP-Triple Quadrupole, MALDI TOF/TOF, LC-QTOF, etc) and protein profiling technologies like multidimensional protein identification technology (MudPIT), differential 2-D (fluorescence) gel electrophoresis (DICE), and Multiplexed Isobaric Tagging Technology for Relative and Absolute Quantitation (iTRAQ¿). These technologies would also be used in conjunction with the more recently established phosphoprotein profiling methodologies and other protein post translational modification characterization tools. Additionally, this resource will also make available its recently developed clinical proteomics tools for absolute quantitation determination of biomarkers or known biological agents in clinically relevant samples to all NBC investigators. (2) Amino Acid Analysis based on ion-exchange chromatography with post-column ninhydrin detection. (3) Protein Sequencing using Edman (N-terminal) sequencers. (4) A Biophysics Section which offers extensive services including HPLC Size Exclusion Chromatography/laser light scattering determination of native protein molecular weights, (5) Large Scale Peptide Synthesis based on tBOC Chemistry. (6) Small Scale Peptide Synthesis based on Fmoc chemistry with custom peptide modifications. (7) Biostatistics Section offering extensive analysis of proteomic data and improved quantitation algorithms to determine disease biomarkers from mass spectrometric data. With its state-of-the-art instrumentation, and very diverse set of proteomic services, the NBC Proteomics Core staff will consult with members of the NBC regarding the optimum technology to best support the proposed research, experimental design, and interpretation of data produced either by the Proteomics Core or other laboratories to meet the specialized needs of NBC investigators - who will be given the same turn-around priority as Yale investigators

生物防御和新发传染病研究项目区域卓越中心（RCE）， 东北生物防御中心（NBC）是一个生物医学研究项目，其核心是支持 多方面的研究和开发活动。作为该计划的一部分，NBC蛋白质组学核心将 在W. M.耶鲁大学凯克基金会生物技术资源实验室， 继续提供非常广泛的生物分析服务，以支持基础科学、临床前和 NBC的临床研究项目此外，在国家生物防御或新出现的传染病时期， 疾病紧急情况下，NBC蛋白质组学核心将准备和投入其所有的大量资源 迎接挑战。NBC蛋白质组学核心将从其所有蛋白质组学中提供这些服务 部分：（1）质谱和蛋白质分析资源，利用最先进的质量 光谱仪器（LC-FT-ICR、LC-LTQ-Orbitrap、LC-QTRAP-Triple Quadrupole、MALDI TOF/TOF、LC-QTOF等）和蛋白质谱分析技术如多维蛋白质鉴定 技术（MudPIT）、差异2-D（荧光）凝胶电泳（DICE）和多重等压 相对和绝对定量标记技术（iTRAQ）。这些技术也将 与最近建立的磷蛋白谱分析方法和其他方法结合使用 蛋白质翻译后修饰表征工具。此外，该资源还将使 可利用其最近开发的临床蛋白质组学工具， 向所有NBC研究者提供临床相关样本中的生物标志物或已知生物制剂。(2)氨基酸 基于离子交换色谱和柱后茚三酮检测的分析。(3)蛋白 使用Edman（N-末端）测序仪进行测序。(4)提供广泛服务的生物物理科 包括HPLC尺寸排阻色谱法/激光散射测定天然蛋白质 （5）基于tBOC化学的大规模肽合成。(6)小规模肽 基于具有定制肽修饰的Fmoc化学的合成。(7)生物统计科提供 广泛分析蛋白质组数据和改进的定量算法，以确定疾病生物标志物 质谱数据。凭借其最先进的仪器和非常多样化的蛋白质组学 服务，NBC蛋白质组学核心工作人员将与NBC的成员就最佳 技术，以最好地支持拟议的研究，实验设计，并产生的数据解释 无论是由蛋白质组学核心或其他实验室，以满足NBC调查人员的专业需求-谁 将获得与耶鲁大学调查员相同的优先权