LTQ-Orbitrap Mass Spectrometer for Yale University Keck Laboratory

耶鲁大学 Keck 实验室的 LTQ-Orbitrap 质谱仪

• 批准号: 7389417
• 负责人: EROL E GULCICEK
• 金额: $ 50万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389417
• 关键词: Academic Medical Centers; Anthrax disease; Arts; Asthma; Biomedical Research; Biotechnology; Blood capillaries; Cells; Characteristics; Charge; Complex; Core Facility; Coupled; Data; Detection; Disease; Drug Addiction; Funding; Grant; High Pressure Liquid Chromatography; Housing; Hybrids; Ions; Kidney Diseases; Laboratories; Lipids; Mass Spectrum Analysis; Mental Depression; Methodology; Minor; National Institute of Drug Abuse; Numbers; Phosphopeptides; Phosphoproteins; Phosphorylation; Play; Post-Translational Protein Processing; Premature Birth; Proteins; Proteomics; Proteomics Shared Resource; Range; Request for Applications; Research; Research Personnel; Research Project Grants; Resolution; Resources; Role; Scanning; Schizophrenia; Services; Substance abuse problem; System; Technology; Tissues; United States National Institutes of Health; Universities; Yale Cancer Center; base; biodefense; capillary; interest; mass spectrometer; melanoma; member; microbial; nanoscale; pathogen; protein function

DESCRIPTION (provided by applicant): This application requests funding for a state-of-the-art shared hybrid mass spectrometer that will provide collaborative research and core service usage for the Yale University, W.M. Keck Biotechnology Resource Laboratory's MS & Proteomics Core. The LTQ-Orbitrap hybrid mass spectrometer manufactured by Thermo Fisher Scientific combines a linear quadrupole ion-trap with the orbitrap mass spectrometer. When this requested mass spectrometer is utilized with our in-house developed phosphopeptide enrichment methodologies and an existing, "inert" capillary nano scale HPLC system, the resulting platform and the applied methodologies will provide what we believe to be the first robust, dedicated phosphoprotein profiling and related phosphoproteomics services available from a major academic core facility. The LTQ-Orbitrap mass spectrometry platform has a unique, MS2 and MS3 data dependent phosphopeptide acquisition capability that is tightly coupled with robust, highly sensitive, fast scanning, high resolution, high mass accuracy, and wide dynamic range characteristics that together create an unmatched ability to confidently detect and identify large numbers of phosphopeptides from highly complex, digested cell and tissue protein extracts of interest. In addition, the platform also offers detection and identification of other protein post-translational modifications, lipids, metabolites, and other types of analyses not currently offered by the Keck Laboratory. Because of these unique capabilities and the important role that phosphorylation plays in controlling protein function, there is very strong interest in providing the Keck Laboratory with this platform so it can be made available on a service charge basis to support 33 biomedical research projects overseen by 26 major and five minor users from 13 universities and medical centers. These users include members of four NIH Centers that are closely associated with the Keck Laboratory and that have strong proteomics components (i.e., Yale/NHLBI Proteomics Center, Yale/NIDA Neuroproteomics Center, the Proteomics Shared Resource in the NCI-funded Yale Cancer Center, and the Proteomics Core of the NIAID-funded Northeast Center of Excellence for Biodefense). Two of the major users, Richard Lifton (NHLBI) and Angus Nairn (NIDA), will collaborate with our center to pursue their research applications in renal disease and in drug addiction and depression while also collaborating on biotechnology research directed at further advancing our highly promising phosphoproteomics technologies. This effort will then serve as the basis for rapidly developing new phosphoproteomics services that will be made available to all other research contributors and users of the Keck Laboratory. Relevance: If this grant is funded, the LTQ-Orbitrap system would make a unique contribution to biomedical research that would extend to investigators studying fundamental mechanisms of diseases including schizophrenia, depression, substance abuse, preterm birth, asthma, melanoma, renal diseases, and the effects of biodefense related microbial pathogens such as anthrax.

描述（由申请人提供）：本申请要求资助最先进的共享混合质谱仪，该质谱仪将为耶鲁大学W.M.凯克生物技术资源实验室的质谱和蛋白质组学核心提供合作研究和核心服务。由赛默飞世尔科技公司生产的LTQ-Orbitrap混合质谱仪结合了线性四极离子阱和orbitrap质谱仪。当这个要求的质谱仪与我们内部开发的磷酸肽富集方法和现有的“惰性”毛细管纳米级高效液相色谱系统一起使用时，由此产生的平台和应用的方法将提供我们认为是第一个强大的，专用的磷酸蛋白分析和相关的磷酸蛋白质组学服务，可以从一个主要的学术核心设施中获得。LTQ-Orbitrap质谱平台具有独特的MS2和MS3数据依赖的磷酸肽采集能力，与强大、高灵敏度、快速扫描、高分辨率、高质量精度和宽动态范围特性紧密结合，共同创造了无与伦比的能力，可以自信地从高度复杂、消化的细胞和组织蛋白提取物中检测和识别大量磷酸肽。此外，该平台还提供检测和鉴定其他蛋白质翻译后修饰，脂质，代谢物和其他类型的分析，目前不提供Keck实验室。由于这些独特的功能和磷酸化在控制蛋白质功能中发挥的重要作用，Keck实验室非常有兴趣为这个平台提供服务，这样它就可以在收费的基础上支持来自13所大学和医学中心的26个主要用户和5个次要用户监督的33个生物医学研究项目。这些用户包括四个NIH中心的成员，这些中心与Keck实验室密切相关，并且具有强大的蛋白质组学成分（即耶鲁/NHLBI蛋白质组学中心，耶鲁/NIDA神经蛋白质组学中心，nci资助的耶鲁癌症中心的蛋白质组学共享资源，以及niaid资助的东北生物防御卓越中心的蛋白质组学核心）。两位主要用户Richard Lifton （NHLBI）和Angus Nairn （NIDA）将与我们的中心合作，继续他们在肾脏疾病、药物成瘾和抑郁症方面的研究应用，同时也将合作进行生物技术研究，以进一步推进我们极具前景的磷蛋白质组学技术。这项工作将作为快速开发新的磷蛋白质组学服务的基础，这些服务将提供给凯克实验室的所有其他研究贡献者和用户。