Mass Spectrometry and Biological Structure

质谱学核心已更名为质谱学和生物结构(MSBS) 核心，以反映蛋白质结构分析日益重要。核磁共振专家雷切尔教授 Klevit博士和硅蛋白结构预测(PSP)专家Lars博士已加入核心 马尔姆斯特罗姆。质谱学专家仍然是大卫·古德利特教授。因此，核心保持其 最初的目标是提供定性和定量的蛋白质组学分析，蛋白质翻译后 修饰分析，以及脂类A结构分析。然而，我们注意到，经修订的核心部分致力于 涉及分析方法的应用，而不是开发原始核心是 订婚了。我们将利用我们的生物分析专业知识代表NWRCE实现以下具体目标 调查人员： 1)确定蛋白质和蛋白质复合体的结构； 2)确定小分子的结构和筛选文库； 3)描述全球蛋白质调控的特征；以及 4)就目标1-3咨询和培训NWRCE调查人员。 为了实现这些目标，我们将利用一些技术，包括但不限于： 1)高效液相色谱-电喷雾电离-串联质谱法。 2)基质辅助激光解吸电离飞行时间质谱仪。 3)核磁共振波谱。 4)支持数据解释的计算方法。

The Mass Spectrometry core has been renamed the Mass Spectrometry and Biological Structure (MSBS) Core to reflect increased importance of protein structure analysis. An expert in NMR, Professor Rachel Klevit, has been added to the core as well as an expert in silico protein structure prediction (PSP), Dr Lars Malmstrom. The mass Spectrometry expert remains Professor David Goodlett. As such the core maintains its original goals of providing qualitative and quantitative proteomic analysis, protein post-translational modification analysis, as well as lipid A structure analysis. However, we note that the revised core is devoted to applications of analytical methods as opposed to development of methods in which the original core was engaged. We will use our bioanalytical expertise to conduct the following specific aims on behalf of NWRCE investigators: 1) Determine structures of proteins and protein complexes; 2) Determine structures of small molecules and screen libraries; 3) Characterize Global Protein Regulation; and 4) Consult and Train NWRCE Investigators on Aims 1 - 3. To carry out these aims we will utilize a number of technologies including, but not limited to, the following: 1) High Performance Liquid Chromatography - Electrospray lonization - Tandem Mass Spectrometry. 2) Matrix Assisted Laser Desorption lonization Time-of-Flight Mass Spectrometry. 3) Nuclear Magnetic Resonance Spectroscopy. 4) Computational methods to support interpretation of data.