Mass Spectrometry and Biological Structure

质谱和生物结构

基本信息

批准号：
7675901
负责人：
DAVID ROBINSON GOODLETT
金额：
$ 27.59万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-20 至 2014-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7675901
关键词：
Algorithms Area Arts Biochemical Biological Biotinylation Budgets Burkholderia mallei Burkholderia pseudomallei Cells Centers of Research Excellence Chemicals Communicable Diseases Computer Simulation Computing Methodologies Consult Data Databases Dissociation Disulfides Electrons Emerging Communicable Diseases Escherichia coli Fourier Transform Fractionation Francisella tularensis Genetic Goals High Pressure Liquid Chromatography Hybrids Ions Laboratories Lasers Libraries Lipid A Lipids Maps Mass Spectrum Analysis Measurement Methods NMR Spectroscopy Nuclear Magnetic Resonance Organism Peptides Pica Disease Post Translational Modification Analysis Post-Translational Protein Processing Problem Solving Protein Analysis Proteins Proteome Proteomics Regulation Research Personnel Research Project Grants Role Salmonella typhimurium Sampling Sequence Analysis Stable Isotope Labeling Structure Surface Technology Time TimeLine Training Travel Vaccine Adjuvant Virulence Yersinia pestis analytical method base biodefense crosslink gastrointestinal homoserine lactone interest ion mobility mass spectrometer method development multi-photon novel vaccines pathogen professor protein complex protein structure protein structure prediction research study small molecule tandem mass spectrometry therapeutic target

项目摘要

The Mass Spectrometry core has been renamed the Mass Spectrometry and Biological Structure (MSBS) Core to reflect increased importance of protein structure analysis. An expert in NMR, Professor Rachel Klevit, has been added to the core as well as an expert in silico protein structure prediction (PSP), Dr Lars Malmstrom. The mass Spectrometry expert remains Professor David Goodlett. As such the core maintains its original goals of providing qualitative and quantitative proteomic analysis, protein post-translational modification analysis, as well as lipid A structure analysis. However, we note that the revised core is devoted to applications of analytical methods as opposed to development of methods in which the original core was engaged. We will use our bioanalytical expertise to conduct the following specific aims on behalf of NWRCE investigators: 1) Determine structures of proteins and protein complexes; 2) Determine structures of small molecules and screen libraries; 3) Characterize Global Protein Regulation; and 4) Consult and Train NWRCE Investigators on Aims 1 - 3. To carry out these aims we will utilize a number of technologies including, but not limited to, the following: 1) High Performance Liquid Chromatography - Electrospray lonization - Tandem Mass Spectrometry. 2) Matrix Assisted Laser Desorption lonization Time-of-Flight Mass Spectrometry. 3) Nuclear Magnetic Resonance Spectroscopy. 4) Computational methods to support interpretation of data.

质谱核心已重命名为质谱和生物结构（MSB）核心以反映蛋白质结构分析的重要性。 NMR的专家，瑞秋教授 klevit已被添加到核心以及硅蛋白结构预测（PSP）的专家，Lars博士马尔姆斯特罗姆。质谱专家仍然是戴维·古德利特（David Goodlett）教授。因此，核心保持了其提供定性和定量蛋白质组学分析，蛋白质后翻译的原始目标修改分析以及脂质A结构分析。但是，我们注意到修订后的核心是专门的对于分析方法的应用，而不是开发原始核心的方法已订婚的。我们将使用我们的生物分析专业知识代表NWRCE进行以下特定目标调查人员： 1）确定蛋白质和蛋白质复合物的结构； 2）确定小分子和屏幕文库的结构； 3）表征全球蛋白质调节；和 4）就目标1-3咨询和培训调查人员。为了实现这些目标，我们将利用许多技术，包括但不限于以下技术： 1）高性能液相色谱 - 电喷雾孤独 - 串联质谱法。 2）矩阵辅助激光解吸孤独飞机质谱。 3）核磁共振光谱。 4）支持数据解释的计算方法。