Mass Spectrometry and Biological Structure

质谱和生物结构

• 批准号: 7675901
• 负责人: DAVID ROBINSON GOODLETT
• 金额: $ 27.59万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-20 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7675901
• 关键词: Algorithms; Area; Arts; Biochemical; Biological; Biotinylation; Budgets; Burkholderia mallei; Burkholderia pseudomallei; Cells; Centers of Research Excellence; Chemicals; Communicable Diseases; Computer Simulation; Computing Methodologies; Consult; Data; Databases; Dissociation; Disulfides; Electrons; Emerging Communicable Diseases; Escherichia coli; Fourier Transform; Fractionation; Francisella tularensis; Genetic; Goals; High Pressure Liquid Chromatography; Hybrids; Ions; Laboratories; Lasers; Libraries; Lipid A; Lipids; Maps; Mass Spectrum Analysis; Measurement; Methods; NMR Spectroscopy; Nuclear Magnetic Resonance; Organism; Peptides; Pica Disease; Post Translational Modification Analysis; Post-Translational Protein Processing; Problem Solving; Protein Analysis; Proteins; Proteome; Proteomics; Regulation; Research Personnel; Research Project Grants; Role; Salmonella typhimurium; Sampling; Sequence Analysis; Stable Isotope Labeling; Structure; Surface; Technology; Time; TimeLine; Training; Travel; Vaccine Adjuvant; Virulence; Yersinia pestis; analytical method; base; biodefense; crosslink; gastrointestinal; homoserine lactone; interest; ion mobility; mass spectrometer; method development; multi-photon; novel vaccines; pathogen; professor; protein complex; protein structure; protein structure prediction; research study; small molecule; tandem mass spectrometry; therapeutic target

The Mass Spectrometry core has been renamed the Mass Spectrometry and Biological Structure (MSBS) Core to reflect increased importance of protein structure analysis. An expert in NMR, Professor Rachel Klevit, has been added to the core as well as an expert in silico protein structure prediction (PSP), Dr Lars Malmstrom. The mass Spectrometry expert remains Professor David Goodlett. As such the core maintains its original goals of providing qualitative and quantitative proteomic analysis, protein post-translational modification analysis, as well as lipid A structure analysis. However, we note that the revised core is devoted to applications of analytical methods as opposed to development of methods in which the original core was engaged. We will use our bioanalytical expertise to conduct the following specific aims on behalf of NWRCE investigators: 1) Determine structures of proteins and protein complexes; 2) Determine structures of small molecules and screen libraries; 3) Characterize Global Protein Regulation; and 4) Consult and Train NWRCE Investigators on Aims 1 - 3. To carry out these aims we will utilize a number of technologies including, but not limited to, the following: 1) High Performance Liquid Chromatography - Electrospray lonization - Tandem Mass Spectrometry. 2) Matrix Assisted Laser Desorption lonization Time-of-Flight Mass Spectrometry. 3) Nuclear Magnetic Resonance Spectroscopy. 4) Computational methods to support interpretation of data.

质谱核心已更名为质谱和生物结构（MSBS） 核心反映了蛋白质结构分析的重要性。核磁共振的专家瑞秋教授 Klevit和计算机蛋白质结构预测（PSP）专家Lars博士也加入了核心 马姆斯壮质谱专家仍然是大卫古德利特教授。因此，核心保持其 最初的目标是提供定性和定量的蛋白质组学分析，蛋白质翻译后 修饰分析以及脂质A结构分析。然而，我们注意到，经修订的核心预算 分析方法的应用，而不是发展的方法，其中原来的核心是 订婚了我们将利用我们的生物分析专业知识代表NWRCE实现以下具体目标 研究者： 1)确定蛋白质和蛋白质复合物的结构; 2)确定小分子的结构并筛选文库; 3)表征全球蛋白质调节;和 4)就目标1 - 3咨询和培训NWRCE调查员。 为了实现这些目标，我们将使用多种技术，包括但不限于以下内容： 1)高效液相色谱-电喷雾电离-串联质谱。 2)基质辅助激光解吸电离飞行时间质谱。 3)核磁共振光谱。 4)支持数据解释的计算方法。