权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mechanisms of Articular Cartilage Lubrication

关节软骨润滑的机制

基本信息

批准号：
7903846
负责人：
Robert L Sah
金额：
$ 18.4万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-18 至 2011-09-17
项目状态：
已结题

项目摘要

Articular cartilage is one of a number of tissues in the body that bears load and slides relative to an apposing tissue surface with remarkable tribological properties¿low friction, low wear, and multiple modes of lubrication acting to lower friction and/or wear. However, in aging and osteoarthritis, the articular surface becomes roughened and eroded. Such deterioration may be the result of several factors leading to excessive loading, a failure in lubrication mechanisms, or both. The broad, long-term objectives are to contribute to the understanding of the mechanobiological system of the interacting joint compartments in order to gain insight into the mechanisms and consequences of lubrication of articular cartilage in joints. This proposal seeks to advance the understanding of lubrication mechanisms, in particular the molecular basis for boundary-mode lubrication of articular cartilage by synovial fluid. The overall hypothesis to be tested is that boundary lubrication of articular cartilage occurs via interactions of synovial fluid molecules with the articular surface, reducing boundary-mode friction and wear, and thereby contributing to the mechanical maintenance of healthyjoints. This hypothesis suggests that the concentration of certain molecules within the joint cavity and their adherence at the articular surface are critical determinants of functional lubrication. The specific hypotheses to be tested are: Hypothesis 1: The SF components that lower friction at a cartilage-cartilage interface via boundary lubrication are Lub/SZP, SAPL, and HA, acting alone or in combination. Hypothesis 2: The SF components that lower wear at a cartilage-cartilage interface via boundary lubrication are Lub/SZP, SAPL, and HA, acting alone or in combination. Hypothesis 3: Solutions of biosynthetic and actual SF provide articular cartilage with friction and wear properties according to levels of Lub/SZP, SAPL, and HA, and results of the studies addressing Hypotheses 1 and 2. To address each hypothesis, studies are proposed beginning with tissue-level studies on excised osteochondral fragments in a cartilage-on-cartilage test configuration. They proceed to selected joint-scale biomechanical experiments on articulating cartilage. Relevance to Public Health: Confirmation of the above hypotheses is relevant both to the normal maintenance of joint health and to pathogenic processes in arthritic disease. Such information may ultimately facilitate diagnostic analysis and therapeutic manipulation of molecules of mechanical importance in the synovial fluid compartment.

关节软骨是体内承受负荷并相对于关节滑动的众多组织之一。具有显着摩擦学特性的贴合组织表面——低摩擦、低磨损、多种摩擦模式润滑可降低摩擦和/或磨损。然而，在衰老和骨关节炎中，关节面变得粗糙并被侵蚀。这种恶化可能是多种因素导致的结果负载、润滑机制故障或两者兼而有之。广泛的长期目标是为了解相互作用的关节室的机械生物学系统，以获得洞察力研究关节中关节软骨润滑的机制和后果。该提案旨在促进对润滑机制的理解，特别是边界模式的分子基础通过滑液润滑关节软骨。要测试的总体假设是关节软骨的边界润滑是通过相互作用发生的滑液分子与关节表面的相互作用，减少边界模式摩擦和磨损，从而有助于健康关节的机械维护。该假设表明浓度关节腔内某些分子的组成及其在关节表面的粘附是关键的决定因素的功能性润滑。待检验的具体假设为：假设 1：SF 成分通过边界润滑降低软骨-软骨界面的摩擦是 Lub/SZP、SAPL 和 HA，单独或联合作用。假设 2：SF 组件通过边界润滑降低软骨-软骨界面的磨损是 Lub/SZP、SAPL 和 HA，单独或联合作用。假设 3：生物合成溶液和实际 SF 为关节软骨提供摩擦和磨损根据 Lub/SZP、SAPL 和 HA 水平确定的特性，以及研究结果假设1和2。为了解决每个假设，建议从切除的组织水平研究开始进行研究软骨对软骨测试配置中的骨软骨碎片。他们继续选择联合规模关节软骨的生物力学实验。与公共卫生的相关性：上述假设的证实与正常情况相关维持关节健康和关节炎疾病的致病过程。此类信息最终可能促进对机械重要性分子的诊断分析和治疗操作滑液室。