软骨基因表达的调控-猫眼课题宝

软骨基因表达的调控

批准号：

7847192

负责人：

LINDA J SANDELL

金额：

$1.61万

依托单位：

WASHINGTON UNIVERSITY

依托单位国家：

美国

项目类别：

财政年份：

2009

资助国家：

美国

项目状态：

已结题

起止时间：

2009-06-10 至 2010-09-30

来源：

https://reporter.nih.gov/project-details/7847192

关键词：

Address Adipose tissue Adult Alternative Splicing Applications Grants Binding Biological Models CCAAT-Enhancer-Binding Proteins Cartilage Cell Line Cells Chick Embryo Chondrocytes Chondrogenesis Clinical Collaborations Collagen Gene Data Degenerative polyarthritis Development Disease Dorsal EWS/FLI 1 Type 1 antisense oligonucleotide Embryo Event Excision Exons Extracellular Matrix Family member Gene Expression Gene Expression Regulation Genes Genetic Transcription Grant Health In Vitro Inflammation Inflammatory Interleukin-1 Intervertebral disc structure Laboratories Length Maintenance Mediating Mesenchymal Messenger RNA NF-kappa B Nuclear Nuclear RNA Osteogenesis Play Process Procollagen Production Protein Biosynthesis Protein Isoforms Proteins RNA Splicing RNA-Binding Proteins Regulation Reporter Role Signal Transduction Skeletal Development Staging Stem cells System TFAP2A gene Testing Tissue Engineering Trans-Activators Transcription Process Translations Type II Procollagen Undifferentiated Work Wound Healing Xenopus adult stem cell bone morphogenetic protein 2 bone morphogenetic protein 4 cartilage development cytokine enhancer-binding protein AP-2 improved in vitro testing in vivo insight mRNA Precursor notochord novel strategies repaired response transcription factor

项目摘要

项目成果

LINDA J SANDELL的其他基金

相似基金

相关文献

中文摘要

翻译

点击翻译按钮获取中文摘要

英文摘要

DESCRIPTION (provided by applicant): Understanding the mechanisms that control chondrogenesis is of major clinical importance for improving tissue engineering strategies that can be applied to repair cartilage during osteoarthritis, for example. Studies proposed for the grant renewal period are focused on deciphering the regulation chondrogenesis at the level of gene transcription, precursor messenger RNA (pre-mRNA) alternative splicing and protein synthesis. The following three Specific Aims will directly address these aspects of cartilage development: 1) Investigate the role of transcription factors during chondrogenesis, specifically AP-2, dEF-1 and C/EBP; 2) Investigate the regulation of the type II procollagen pre-mRNA switch that occurs during chondrogenesis and 3) Determine the function of the developmentally-expressed type IIA procollagen protein isoform in vivo. Combined data from these Specific Aims will provide unique insight into the regulation of chondrogenesis as it is becoming apparent that processes of transcription, RNA splicing and translation are tightly coordinated. We also plan to study mechanisms important for cartilage maintenance and repair as described in the fourth Specific Aim: 4) Investigate the mechanism of IL-1b and TNF-a induction of BMP-2 in adult chondrocytes. Processes that occur during chondrogenesis are believed to be recapitulated during tissue repair under conditions of cartilage degradation. Therefore, the final Specific Aim in combination with the first three Specific Aims focused on chondrogenesis will provide invaluable information into the maintenance of cartilage health. The predominant experimental approach to these topics will be to use in vitro systems of chondrogenesis utilizing the ATDC5 cell line and adult stem cells isolated from adipose tissue to investigate state-specific differentiation events. Regulatory factors will be tested by over expression and inhibition studies and correlated with expression in vivo.

期刊论文(42)

专著(0)

科研奖励(0)

会议论文