Regulation and Function of Keratins in the Epidermis

表皮角蛋白的调节和功能

• 批准号: 7847960
• 负责人: Dennis Roop
• 金额: $ 3.56万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-10 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7847960
• 关键词: Address; Affect; Biology; Bullous Congenital Ichthyosiform Erythroderma; C-terminal; Cell Cycle; Commit; Cytokeratin filaments; Cytoskeleton; Desmosomes; Development; Disease; Embryo; Epidermis; Epithelial; Funding; Genes; Genetic; Grant; In Vitro; Individual; Inherited; Intermediate Filaments; Keratin; Lead; Mechanical Stress; Mitotic; Modeling; Molecular; Mutation; Protein Biosynthesis; Protein Isoforms; Proteins; Regulation; Research; Role; Staging; Stratification; Structure; Surface Ectoderm; Testing; Tissues; Transgenic Mice; Transgenic Organisms; base; cell envelope; cell type; gene therapy; improved; in vivo; insight; keratinocyte; mouse model; novel; novel therapeutic intervention; programs; retinal rods; skin disorder; transcription factor

DESCRIPTION (provided by applicant): With the discovery that mutations in fourteen different keratin genes can cause eight distinct epithelial fragility disorders, comes the realization that the keratin filament cytoskeleton is crucial to the structural integrity of epithelial tissues exposed to mechanical stress. The identification of mutations causing these disorders has improved our understanding of keratin structure and function, particularly with regard to the highly conserved regions of the central rod domain. However, two central questions in keratin biology still remain. The first question concerns the regulation of individual keratin genes, since the tightly regulated synthesis of these proteins is a pre-requisite for the normal development of the epidermis. To address this question, we will focus on two keratin genes that are expressed at different stages of epidermal development, keratins K14 and K1. K14 is one of the first genes expressed in the surface ectoderm after commitment to stratification. We have recently demonstrated that the transcription factor p63 is the molecular switch for initiation of the epidermal stratification program. This discovery has provided us with novel insights into the molecular mechanisms responsible for the induction of K14 expression during epidermal development. We have also discovered that a switch in p63 isoform expression is required to allow basal keratinocytes to withdraw from the cell cycle and commit to terminal differentiation. Since K1 is one of the first genes expressed in the epidermis after keratinocytes have committed to terminal differentiation, we will exploit this discovery to gain insight into the molecular mechanisms regulating K1 expression during maturation of the epidermis. The second question concerns the roles of the keratin N- and C-terminal end domains. These domains are distinctive for each keratin protein but are remarkably well conserved across species and presumably have functional significance. In vitro studies have suggested that these domains interact with desmosomes and/or the cell envelope, however very little is known regarding cell type-specific functions of these domains in vivo. Two transgenic approaches are proposed to address this question. Finally, epidermolytic hyperkeratosis (EHK), the dominantly inherited skin disorder caused by mutations in the post-mitotically expressed keratins, K1 or K10, presents a challenge for gene therapy. During the last funding period of this grant, we generated a mouse model that mimics EHK at both the genetic and phenotypic level. This mouse model has provided new insights into the molecular and cellular basis of EHK and we propose to use this model to test new gene therapy strategies. The results of this research will yield valuable information that can lead to novel therapeutic approaches for other diseases affecting post-mitotic keratinocytes.

描述（由申请人提供）：随着发现14种不同角蛋白基因的突变可导致8种不同的上皮易碎性疾病，人们认识到角蛋白丝细胞骨架对暴露于机械应力下的上皮组织的结构完整性至关重要。对引起这些疾病的突变的鉴定提高了我们对角蛋白结构和功能的理解，特别是关于中央杆结构域的高度保守区域。然而，角蛋白生物学的两个核心问题仍然存在。第一个问题涉及单个角蛋白基因的调控，因为这些蛋白质的严格调控合成是表皮正常发育的先决条件。为了解决这个问题，我们将重点关注在表皮发育的不同阶段表达的两个角蛋白基因，角蛋白K14和K1。K14是细胞层化后最早在外胚层表达的基因之一。我们最近已经证明转录因子p63是表皮分层程序启动的分子开关。这一发现为我们在表皮发育过程中诱导K14表达的分子机制提供了新的见解。我们还发现，p63异构体表达的一个开关是允许基底角质形成细胞退出细胞周期并致力于终端分化所必需的。由于K1是角化细胞最终分化后表皮中最早表达的基因之一，我们将利用这一发现来深入了解表皮成熟过程中调节K1表达的分子机制。第二个问题涉及角蛋白N端和c端结构域的作用。这些结构域对于每个角蛋白都是独特的，但在物种之间非常保守，可能具有功能意义。体外研究表明，这些结构域与桥粒和/或细胞包膜相互作用，然而，对于这些结构域在体内的细胞类型特异性功能知之甚少。提出了两种转基因方法来解决这个问题。最后，表皮松解性角化过度症（EHK）是一种主要由有丝分裂后表达角蛋白K1或K10突变引起的遗传性皮肤病，对基因治疗提出了挑战。在这项资助的最后一个资助期间，我们建立了一个小鼠模型，在遗传和表型水平上都模仿了EHK。该小鼠模型为EHK的分子和细胞基础提供了新的见解，我们建议使用该模型来测试新的基因治疗策略。这项研究的结果将产生有价值的信息，可以导致新的治疗方法影响有丝分裂后角质形成细胞的其他疾病。