Functional Classification of Cardiomyocytes Derived from Stem Cells
干细胞来源的心肌细胞的功能分类
基本信息
- 批准号:8259042
- 负责人:
- 金额:$ 20.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-04-20 至 2014-03-31
- 项目状态:已结题
- 来源:
- 关键词:Action PotentialsAddressAdipocytesAdrenergic AgentsAlgorithmsArrhythmiaBlood CellsBone MarrowCardiacCardiac MyocytesCause of DeathCell Culture TechniquesCellsCharacteristicsClassificationDataData SetDescriptorDevelopmentDevicesDiscriminant AnalysisElectrophysiology (science)EmbryoEvaluationEvolutionFaceFrequenciesFutureGoalsHeartHeart AtriumHeart DiseasesIsometric ExerciseMachine LearningMapsMeasurementMethodsMicroelectrodesMorphologyMyocardiumNatural regenerationNodalOpticsPharmaceutical PreparationsPhenotypePrincipal Component AnalysisProceduresPropertyRegenerative MedicineResearchResearch PersonnelResourcesRestRiskSeriesShapesStagingStem cellsTechniquesTestingTimeTissuesTrainingTransplantationUmbilical Cord BloodUnited StatesVariantVentricularWomanadrenergicadult stem cellbasecardiac repaircell typecholinergicclinical applicationfunctional lossheart cellhuman embryonic stem cellhuman stem cellsinduced pluripotent stem cellinnovationinterestmenmolecular markernovelpublic health relevanceresearch studyresponsestem cell therapytissue repair
项目摘要
DESCRIPTION (provided by applicant): Heart disease is the number one cause of death in the United States each year for both women and men. Although significant advances have been made in conventional drug and device therapies in recent years, they have not been able to reverse the loss of functional myocardium. Regeneration of the heart may someday be possible with the ability to derive functional cardiomyocytes from human stem cells. However, before these cells can be used for cardiac repair, more must be known about their electrophysiology and their likelihood to seamlessly integrate with native cardiac tissue. In particular, it is of critical importance to establish the electrophysiological compatibility of these cells with host myocardium to minimize the risk of arrhythmia. Despite this critical need, the classification of the electrophysiological phenotypes has been largely subjective for all cell types that have been studied so far, relying mainly on parameters related to action potential shape. The overall goal of this project is to develop a new, analytical and automated method to classify newly differentiated cardiac cells, based on techniques developed for machine learning. The specific aims are first, to use optical mapping and microelectrode recordings to generate datasets of functional electrophysiological characteristics of human embryonic stem cell-derived cardiomyocytes (hESC-CMs), and second, to use machine learning techniques to classify the phenotypes of the hESC-CMs, based on parametric descriptions. These techniques will involve linear and nonlinear dimensionality reduction algorithms, and clustering and classification algorithms. Cells at different stages of differentiation will be evaluated at different pacing and pharmacological conditions that will help to establish the functional properties of the cells. In summary, the proposed research will enable the classification of cardiomyocytes that are derived from human stem cells. The ability to classify and identify cardiomyocyte phenotypes will permit a quantitative assessment of the batch-to-batch variability in cell cultures, the effect of different differentiation procedures, and the evolution of phenotypes during cardiomyocyte differentiation and maturation. These are critically important issues for the future clinical application of these cells to regenerate cardiac tissue.
PUBLIC HEALTH RELEVANCE: Stem cell therapy holds great potential for treating diseased and failing hearts, but still faces significant challenges. This project addresses the electrophysiological aspects these cells, and the goal is to develop methods that can classify and identify the variety of heart cells that are derived from different kinds of stem cells.
描述(由申请人提供):心脏病是美国每年女性和男性的第一大死因。尽管近年来传统药物和设备疗法取得了重大进展,但仍无法逆转功能性心肌的丧失。有一天,通过从人类干细胞中提取功能性心肌细胞的能力,心脏的再生可能成为可能。然而,在这些细胞用于心脏修复之前,必须更多地了解它们的电生理学以及它们与天然心脏组织无缝整合的可能性。特别是,建立这些细胞与宿主心肌的电生理相容性对于最大限度地降低心律失常的风险至关重要。尽管存在这一迫切需求,但对于迄今为止研究的所有细胞类型来说,电生理表型的分类在很大程度上是主观的,主要依赖于与动作电位形状相关的参数。 该项目的总体目标是基于机器学习开发的技术,开发一种新的分析自动化方法来对新分化的心脏细胞进行分类。具体目标是,首先,使用光学测绘和微电极记录生成人胚胎干细胞来源的心肌细胞(hESC-CM)功能性电生理特征的数据集,其次,使用机器学习技术根据参数描述对 hESC-CM 的表型进行分类。这些技术将涉及线性和非线性降维算法以及聚类和分类算法。处于不同分化阶段的细胞将在不同的起搏和药理学条件下进行评估,这将有助于确定细胞的功能特性。 总之,拟议的研究将使人类干细胞衍生的心肌细胞分类成为可能。分类和识别心肌细胞表型的能力将允许定量评估细胞培养物中批次间的变异性、不同分化程序的影响以及心肌细胞分化和成熟过程中表型的演变。对于这些细胞未来临床应用再生心脏组织来说,这些都是至关重要的问题。
公众健康相关性:干细胞疗法在治疗患病和衰竭的心脏方面具有巨大潜力,但仍面临重大挑战。该项目解决了这些细胞的电生理学方面的问题,目标是开发可以分类和识别源自不同种类干细胞的各种心脏细胞的方法。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A metamorphosis distance for embryonic cardiac action potential interpolation and classification.
用于胚胎心脏动作电位插值和分类的变态距离。
- DOI:10.1007/978-3-642-40811-3_59
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:Gorospe,Giann;Younes,Laurent;Tung,Leslie;Vidal,René
- 通讯作者:Vidal,René
Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes.
- DOI:10.1186/scrt507
- 发表时间:2014-10-20
- 期刊:
- 影响因子:7.5
- 作者:Zhu R;Blazeski A;Poon E;Costa KD;Tung L;Boheler KR
- 通讯作者:Boheler KR
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
LESLIE TUNG其他文献
LESLIE TUNG的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('LESLIE TUNG', 18)}}的其他基金
Engineered Human Heart Slice for Testing Drug-Induced Arrhythmia
用于测试药物引起的心律失常的工程人体心脏切片
- 批准号:
10593346 - 财政年份:2020
- 资助金额:
$ 20.5万 - 项目类别:
Engineered Human Heart Slice for Testing Drug-Induced Arrhythmia
用于测试药物引起的心律失常的工程人体心脏切片
- 批准号:
10593334 - 财政年份:2020
- 资助金额:
$ 20.5万 - 项目类别:
Engineered Human Heart Slice for Testing Drug-Induced Arrhythmia
用于测试药物引起的心律失常的工程人体心脏切片
- 批准号:
10250777 - 财政年份:2020
- 资助金额:
$ 20.5万 - 项目类别:
Mechanoelectrical Interactions Between Cardiac Myofibroblasts and Myocytes
心脏肌成纤维细胞和肌细胞之间的机电相互作用
- 批准号:
9204715 - 财政年份:2016
- 资助金额:
$ 20.5万 - 项目类别:
ARVD/C Dysfunction in Human Stem Cell-Derived Cardiac Tissue
人类干细胞来源的心脏组织中的 ARVD/C 功能障碍
- 批准号:
9815578 - 财政年份:2016
- 资助金额:
$ 20.5万 - 项目类别:
ARVD/C Dysfunction in Human Stem Cell-Derived Cardiac Tissue
人类干细胞来源的心脏组织中的 ARVD/C 功能障碍
- 批准号:
9106007 - 财政年份:2016
- 资助金额:
$ 20.5万 - 项目类别:
Mechanoelectrical Interactions Between Cardiac Myofibroblasts and Myocytes
心脏肌成纤维细胞和肌细胞之间的机电相互作用
- 批准号:
9028886 - 财政年份:2016
- 资助金额:
$ 20.5万 - 项目类别:
ARVD/C Dysfunction in Human Stem Cell-Derived Cardiac Tissue
人类干细胞来源的心脏组织中的 ARVD/C 功能障碍
- 批准号:
9251893 - 财政年份:2016
- 资助金额:
$ 20.5万 - 项目类别:
Functional Classification of Cardiomyocytes Derived from Stem Cells
干细胞来源的心肌细胞的功能分类
- 批准号:
8095482 - 财政年份:2011
- 资助金额:
$ 20.5万 - 项目类别:
相似海外基金
Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
- 批准号:
MR/S03398X/2 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Fellowship
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
- 批准号:
2338423 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Continuing Grant
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
- 批准号:
EP/Y001486/1 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Research Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
- 批准号:
MR/X03657X/1 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
- 批准号:
2348066 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Standard Grant
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
- 批准号:
2341402 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
- 批准号:
AH/Z505481/1 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
EU-Funded
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
- 批准号:
AH/Z505341/1 - 财政年份:2024
- 资助金额:
$ 20.5万 - 项目类别:
Research Grant














{{item.name}}会员




