JHU Ultima-L Imaging System

JHU Ultima-L 成像系统

• 批准号: 7595518
• 负责人: LESLIE TUNG
• 金额: $ 15.77万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595518
• 关键词: Arrhythmia; Basic Science; Biological; Calcium; Cardiac; Cell Culture Techniques; Cells; Dimensions; Event; Fluorescence; Hair Cells; Image; Measurement; Measures; Microscopic; Optics; Organ; Pathogenesis; Pattern; Pulmonary artery structure; Regulation; Research; Resolution; Signal Transduction; Signaling Molecule; System; Time; Tissues; Universities; Weight; base; digital imaging; instrument; instrumentation; neurodevelopment; public health relevance; release of sequestered calcium ion into cytoplasm

DESCRIPTION (provided by applicant): It is proposed to enhance the research capabilities at the Johns Hopkins University by the addition of a new shared instrument, a fast imaging system from SciMedia for fluorescence measurements in cells and tissues. The dual cameras in the proposed system will allow simultaneous imaging at two optical wavelengths, at frame rates between 1,000 and 5,000 frames per second (fps), or of one probe up to 10,000 fps. Each camera is a MiCAM Ultima-L camera, which has a fast CMOS chip with high spatial resolution that can be used for both microscopic and macroscopic imaging of dynamic optical signals. Existing cameras at Johns Hopkins are weighted towards very high spatial resolution and sensitivity, but they are incapable of measuring signals at frame rates greater than about 500 fps. The SciMedia system is a complete system, with hardware, software and computer for image acquisition and display, and hence, can be easily mastered by new users. Examples of projects that can be undertaken with this system are the study of: calcium-based arrhythmias in cardiac cell cultures, spatio- temporal patterns of calcium-dependent signaling molecules, analysis of cell signaling at fast time scales, calcium flux and vesicular release in cochlear hair cells, calcium regulation in pulmonary arteries, and neural development and pathogenesis. In summary, the proposed instrumentation will fill a major gap in the imaging capabilities for basic science studies at Johns Hopkins and enable new horizons to be explored in a diverse range of health-related studies, as outlined in this proposal. PUBLIC HEALTH RELEVANCE: Major advances in the study of cell and tissue function as made possible by a wide range of optical probes. The proposed instrumentation system will enable a new dimension of imaging studies of fast biological events to be performed at the Johns Hopkins University and will greatly facilitate our understanding of the basis of normal and pathological function of tissues and organs.

描述（由申请人提供）：为了提高约翰霍普金斯大学的研究能力，我们提议增加一种新的共享仪器，一种来自SciMedia的用于细胞和组织荧光测量的快速成像系统。拟议系统中的双摄像头将允许在两个光学波长下同时成像，帧率在1000到5000帧每秒（fps）之间，或者一个探头高达10,000帧每秒。每个相机都是MiCAM Ultima-L相机，该相机具有高空间分辨率的快速CMOS芯片，可用于动态光信号的微观和宏观成像。约翰·霍普金斯大学现有的相机都倾向于极高的空间分辨率和灵敏度，但它们无法测量帧率超过500帧/秒的信号。SciMedia系统是一个完整的系统，有硬件、软件和计算机用于图像采集和显示，因此，新用户很容易掌握。该系统可用于以下研究项目：心脏细胞培养中钙基心律失常、钙依赖信号分子的时空模式、快速时间尺度细胞信号分析、耳蜗毛细胞中的钙通量和囊泡释放、肺动脉中的钙调节、神经发育和发病机制。总之，拟议的仪器将填补约翰·霍普金斯大学基础科学研究成像能力方面的重大空白，并使本提案概述的各种健康相关研究能够开拓新的领域。公共卫生相关性：广泛的光学探针使细胞和组织功能研究取得重大进展。所提出的仪器系统将使约翰霍普金斯大学对快速生物事件的成像研究进入一个新的维度，并将极大地促进我们对组织和器官正常和病理功能基础的理解。

项目成果

Variability of Action Potentials Within and Among Cardiac Cell Clusters Derived from Human Embryonic Stem Cells.

• DOI: 10.1038/srep18544
• 发表时间: 2016-01-05
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Zhu R;Millrod MA;Zambidis ET;Tung L
• 通讯作者: Tung L

Automated grouping of action potentials of human embryonic stem cell-derived cardiomyocytes.

• DOI: 10.1109/tbme.2014.2311387
• 发表时间: 2014-09
• 期刊: IEEE transactions on bio-medical engineering
• 作者: Gorospe G;Zhu R;Millrod MA;Zambidis ET;Tung L;Vidal R
• 通讯作者: Vidal R

Defibrillation success with high frequency electric fields is related to degree and location of conduction block.

高频电场除颤的成功与否与传导阻滞的程度和位置有关。

• DOI: 10.1016/j.hrthm.2013.01.016
• 发表时间: 2013
• 期刊: Heart rhythm
• 影响因子: 5.5
• 作者: Weinberg,SethH;Chang,KellyC;Zhu,Renjun;Tandri,Harikrishna;Berger,RonaldD;Trayanova,NataliaA;Tung,Leslie
• 通讯作者: Tung,Leslie

Electrophysiological and contractile function of cardiomyocytes derived from human embryonic stem cells.

• DOI: 10.1016/j.pbiomolbio.2012.07.012
• 发表时间: 2012-10
• 期刊: Progress in biophysics and molecular biology
• 影响因子: 3.8
• 作者: Blazeski A;Zhu R;Hunter DW;Weinberg SH;Boheler KR;Zambidis ET;Tung L
• 通讯作者: Tung L

Cardiomyocytes derived from human induced pluripotent stem cells as models for normal and diseased cardiac electrophysiology and contractility.

• DOI: 10.1016/j.pbiomolbio.2012.07.013
• 发表时间: 2012-10
• 期刊: Progress in biophysics and molecular biology
• 影响因子: 3.8
• 作者: Blazeski A;Zhu R;Hunter DW;Weinberg SH;Zambidis ET;Tung L
• 通讯作者: Tung L