INTEGRATED FERROFLUIDIC MICROCHIP FOR CELL MANIPULATION AND ENRICHMENT

用于细胞操作和富集的集成铁磁流体微芯片

• 批准号: 8352207
• 负责人: Ji Chen
• 金额: $ 21.06万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8352207
• 关键词: Abnormal Cell; Adoption; Area; Biological Assay; Biological Markers; Biomedical Research; Cancer Biology; Cancer Control; Caring; Cells; Cellular biology; Centers for Disease Control and Prevention (U.S.); Cervical; Cervical Cancer Screening; Characteristics; Chemistry; Cytopathology; Development; Devices; Effectiveness; Elasticity; Electrodes; Electromagnetics; Electronics; Engineering; Faculty; Future; Goals; Growth; Health Care Costs; Individual; Interdisciplinary Study; Label; Life; Liquid substance; Magnetism; Malignant Neoplasms; Malignant neoplasm of cervix uteri; Methods; Microfabrication; Microfluidics; Molecular; Outcome; Pattern; Prevention; Production; Reproducibility; Research; Research Personnel; Research Project Grants; Sampling; Science; Screening for cancer; Screening procedure; Shapes; Site; Specimen; Strategic Planning; Supporting Cell; Surface; Techniques; Technology; Testing; Time; United States Dept. of Health and Human Services; Universities; Woman; base; cancer cell; cell behavior; cervical cancer prevention; clinically relevant; cost; cost effective; design; disorder prevention; ferrofluid; flexibility; fluorescence activated cell sorter device; graduate student; improved; innovation; magnetic field; microchip; model design; models and simulation; novel; polydimethylsiloxane; prevent; prototype; research study; success; tv watching; two-dimensional; user-friendly

DESCRIPTION (provided by applicant): In this proposal, we aim to develop a fully integrated ferrofluidic microchip to magnetically manipulate and enrich cells inside bio-compatible ferrofluids in a label-free manner by combining microfluidics and dynamically-reconfigurable wire-mesh coils. Goals of this research are (1) to develop the ferrofluidic microchip as a general cell manipulation platform for biomedical research, and (2) to develop a fast and low-cost front-end separation method to enrich cervical abnormal cells for increased reproducibility, reduced screening time, and improved screening accuracy. We plan to achieve these goals through pursuing three specific aims. (1) Development of integrated ferrofluidic cell manipulation and enrichment microchip. We will model, design, fabricate and test a novel and versatile wire-mesh coil to produce reconfigurable and dynamic magnetic field patterns. A PDMS microchannels will be designed and integrated with the wire-mesh coil to form the prototype chip. (2) Demonstration of the chip's effectiveness in cell manipulation. We will develop bio-compatible ferrofluids for live cell experiments. We will exploit size, shape and elasticity of cells as potenial manipulation characteristics. Cell manipulation will be conducted with live cells. (3) Application of chip in cervical cancer cell enrichment. The chip can be applied in areas in cell biology where understanding of cell behavior requires isolation and manipulation of certain cell subpopulations. This is a highly interdisciplinary research project because it deals with the themes of colloidal chemistry, fluid dynamics, magnetism, electronics, modeling and simulation, microfabrication, and cancer biology. An interdisciplinary team in both engineering sciences (University of Georgia and University of Houston) and cancer biology (Centers for Disease Control and Prevention) is formed for this project. The team includes 4 university faculty, one CDC cervical cancer expert, one CDC postdoctoral scholar, and two graduate students. The duration of the proposed project period is 3 years. PUBLIC HEALTH RELEVANCE (provided by applicant): The chip can be applied in areas in cell biology where understanding of cell behavior requires isolation and manipulation of certain cell subpopulations. The chip will be used to enrich abnormal cervical cells and support the effort for better prevention and control of cervical cancer, which is the second most common cancer in women globally. This device supports Department of Health and Human Services objectives in the 2010-2015 Strategic Plan to "reduce the growth of health care costs while promoting high-value, effective care."

描述（由申请人提供）：在本提案中，我们的目标是开发一种完全集成的铁磁流体微芯片，通过结合微流体和动态可重构的线网线圈，以无标记的方式对生物相容性铁磁流体内的细胞进行磁性操纵和富集。这项研究的目标是（1）开发铁磁流体微芯片作为生物医学研究的通用细胞操作平台，以及（2）开发一种快速、低成本的前端分离方法来富集宫颈异常细胞，以提高再现性、减少筛查时间并提高筛查准确性。我们计划通过追求三个具体目标来实现这些目标。 (1) 集成铁磁流体细胞操作和富集微芯片的开发。我们将建模、设计、制造和测试一种新颖的多功能线网线圈，以产生可重新配置的动态磁场模式。 PDMS 微通道将被设计并与线网线圈集成以形成原型芯片。 (2) 验证芯片在细胞操作方面的有效性。我们将开发用于活细胞实验的生物相容性铁磁流体。我们将利用细胞的大小、形状和弹性作为潜在的操纵特征。细胞操作将使用活细胞进行。 (3)芯片在宫颈癌细胞富集中的应用。该芯片可应用于细胞生物学领域，在这些领域中，了解细胞行为需要分离和操作某些细胞亚群。 这是一个高度跨学科的研究项目，因为它涉及胶体化学、流体动力学、磁学、电子学、建模和模拟、微加工和癌症生物学等主题。该项目组建了工程科学（乔治亚大学和休斯顿大学）和癌症生物学（疾病控制与预防中心）的跨学科团队。该团队包括 4 名大学教员、1 名 CDC 宫颈癌专家、1 名 CDC 博士后学者和 2 名研究生。拟建项目工期为3年。 公共健康相关性（由申请人提供）：该芯片可应用于细胞生物学领域，在这些领域中，了解细胞行为需要分离和操作某些细胞亚群。该芯片将用于富集异常宫颈细胞，支持更好地预防和控制宫颈癌，宫颈癌是全球女性第二常见的癌症。该设备支持卫生与公众服务部 2010-2015 年战略计划中的目标，即“减少医疗保健成本的增长，同时促进高价值、有效的护理”。