METAL ION REGULATION CORE ADMINISTRATIVE PAGES

金属离子监管核心管理页面

• 批准号: 8379473
• 负责人: Martina Ralle
• 金额: $ 24.08万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8379473
• 关键词: AA Spectrophotometry; Antibody Formation; Antigens; Baculoviruses; Behavior; Businesses; Carrier Proteins; Categories; Cells; Charge; Communities; Confocal Microscopy; Copper; Core Facility; Data Analyses; Disease; Electrophoresis; Ensure; Equipment; Generations; Goals; Health; Homeostasis; Human; Human Resources; Imagery; Immunohistochemistry; Ion Transport; Ions; Mass Spectrum Analysis; Mediating; Metals; Microscope; Monoclonal Antibodies; Peptides; Protein Analysis; Proteins; Protocols documentation; Reagent; Recombinant Proteins; Regulation; Research; Research Personnel; Selenium; Selenocysteine; Services; Tissues; Training; Wilson disease protein; absorption; instrument; instrumentation; interest; mass spectrometer; mutant; programs; protein expression; research study; trafficking; two-dimensional

The purpose of the Metal Ion Core is to provide the instrumentation, technical support, and to generate reagents necessary to fulfill the goals of this application. The core will provide atomic absorption analysis of metals; equipment and expertise to perform two-dimensional electrophoresis analysis of proteins, a mass spectrometry facility for the analysis of proteins and peptides; and a confocal microscope facility for the immunolocalization of proteins in cells and tissues. The atomic absorption instrument will allow sensitive quantitative analysis of copper and selenium ions necessary to study copper transport and transfer (Projects 1,2, and 4). The mass spectrometer will be used to identify protein interacting with the copper transporters and regulating their intracellular localization and trafficking behavior (Projects 3,4, and 5), as well as to confirm the efficiency of incorporation of selenocysteine into proteins prepared for small angle x-ray diffraction experiments. The confocal microscope will allow direct visualization of the subcellular localization of ion transport proteins so that the mechanisms controlling their trafficking in normal and diseased tissue can be understood (Project 4). The important charge of the core will be to express and purify recombinant proteins for production of antibodies against proteins involved in copper homeostasis and well as to generate proteins for collaborative research between all five projects. These latter category of proteins includes various mutants of ATP7B (Project 5), NBDdelta TMS1,2 (Project 2 and 4), and deltal-63 protein (Projects 4 and 5)

金属离子核心的目的是提供仪器、技术支持和生成试剂