Inductively Coupled Plasma Mass Spectrometer for High Sensitivity Elemental Analysis

项目摘要/摘要 这项提案的长期目标是帮助NIH或其他联邦资助项目的研究人员 更好地理解微量元素在生物学或医学中的作用。元素分析核心(EAC)位于 俄勒冈健康与科学大学(OHSU)分析生物标本中的元素含量 电感耦合等离子体质谱(ICPMS)。作为OHSU的大学共享资源， 然而，EAC主要为来自OHSU和波特兰地区的用户服务，因为我们在 我们还与美国和加拿大的许多调查人员合作进行痕量元素分析。自.以来 选管会于2011年开始，我们已分析了约14,000个样本，并稳步增加每年的 样本装载。我们目前的仪器，一个单四极电感耦合等离子体质谱仪， 是由2009年的S10赠款资助的，不再满足我们的需求，要求我们的大多数用户群。 因此，我们正在申请资金，用安捷伦8900三重四极杆取代这台仪器 具有单粒子选项的电感耦合等离子体质谱仪。这个新的，最先进的 仪器的问世，将极大地改善我们目前的服务，并增加一项全新的技术，元素分析 Signle粒子，到我们的投资组合。它克服了单一四元仪的局限性，如分析 样品的硫含量或钛浓度以及探测器技术的改进提供了10- 灵敏度提高100倍。极大地提高了探测器的速度与增强的灵敏度相结合 允许使用专门的样品导入，一次测量一种粒子的元素浓度 系统。这将使用于确定单个细胞或纳米颗粒中的痕量元素浓度， 举个例子。我们还将制定协议来检测线粒体等亚细胞细胞器中的内容。Dr。 Ralle是EAC的主任，在微量元素的生物分析方面拥有超过25年的经验 在生物学方面；她是国际公认的元素分析专家，并成功地处理了 EAC。正是因为她的专业知识和声誉，来自美国各地的客户将样品送到EAC，以便 对OHSU的分析。目前在太平洋任何地方都没有类似的仪器具有这种设置 西北部、阿拉斯加或北加州。这一新仪器将创建一个强大的元素分析 为全美生物医学研究人员提供的资源。

Project Summary/Abstract The long term objective of this proposal is to help researchers with NIH or other federally funded projects to better understand the role of trace elements in biology or medicine. The Elemental Analysis Core (EAC) at the Oregon Health & Science University (OHSU) analyses biological specimen for their elemental content using Inductively Coupled Plasma Mass Spectrometry (ICPMS). As a University Shared Resource at OHSU, the EAC primarily serves users from OHSU and the Portland Area, however, because of our unique expertise in trace elemental analysis we also collaborate with many investigators across the US and Canada. Since inception of the EAC in 2011 we have analyzed ~14,000 samples and have steadily increased our annual sample load. Our current instrumentation, a single quadrupole inductively coupled plasma mass spectrometer, was funded by an S10 grant in 2009 and no longer meets the needs and asks for a majority of our user base. We are therefore requesting funds to replace this instrument with an Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometer with single particle option. This new, state-of-the-art instrument, will greatly improve our current services and add an entirely new technique, elemental analysis of signle particles, to our portfolio. It overcomes limitations of the single quad instrument such as analyzing samples for their sulfur content or titanium concentration and improvements in detector technology offer a 10 – 100 fold increase in sensitivity. The vastly increased detector speed in combination with enhanced sensitivity allows for measuring elemental concentration for one particle at a time using a specialized sample introduction system. This will enable used to determine concentrations of trace elements in single cells or nanoparticles, for example. We will also develop protocols to detect contents in subcellular organelles such as mitochondria. Dr. Ralle, the Director of the EAC, has over 25 years of experience in the bioanalytical aspects of trace elements in biology; she is an internationally recognized expert for elemental analysis and has successfully managed the EAC. It is because of her expertise and reputation clients from across the US send samples to the EAC for analyses to OHSU. There are currently no comparable instruments with this setup anywhere in the Pacific Northwest, Alaska, or Northern California. This new instrument will create a powerful elemental analysis resource for biomedical researchers across the US.