ICP-MS to Measure Elemental Concentrations

描述(由申请人提供)：在这份申请中，我们请求为OHSU生物化学和分子生物学系的金属离子核心提供一台电感耦合等离子体质谱仪(ICP-MS)。电感耦合等离子体质谱(ICP-MS)测定从Li到U的元素浓度，具有高灵敏度、高质量精度和宽的线性检测范围。由于高质量精度和高灵敏度，测定同位素比率的实验也是可能的。由于这些独特的特性，电感耦合等离子体质谱仪是各种生物医学项目的完美工具，这些项目通常在医学研究机构执行。在与疾病有关的研究项目中，能够可靠地确定生物材料中的元素浓度往往是至关重要的，特别是如果这些疾病是由金属代谢障碍引起的或涉及到这些疾病。在阐明含有金属的酶的功能或对蛋白质进行结构研究时，元素含量的准确测量也是至关重要的。在过去的5年里，OHSU的金属离子核心为多名研究人员提供了与生物材料中金属和元素浓度有关的服务。目前，可用于测定元素浓度的仪器往往是一个限制步骤。电感耦合等离子体质谱仪将使金属离子核的现有和新用户能够在他们的研究中纳入必要的但目前无法获得的测量。我们选择的具体乐器是Perkin Elmer的Elan DRCII。在过去的25年里，珀金·埃尔默一直是电感耦合等离子体质谱仪器的领先制造商。虽然有其他制造商的仪器可用，但它们目前在OHSU等医学院环境中的足迹非常有限，在那里通常会分析过多的不同样本材料。Elan DRCII提供了目前可用的最佳检测限值，同时有效地消除了生物材料中常见的干扰。目前，校园内还没有电感耦合等离子体质谱仪。现有的能够在校园内测定金属浓度的仪器受到线性范围的限制，无法同时测量多个元素，或者它们对大样本量的要求。然而，显然有必要在整个OHSU建立电感耦合等离子体质谱(ICPMS)。来自该校多个学院和院系的研究人员表达了他们对获得ICP-MS的兴趣和大力支持。公共卫生相关性各种严重的疾病要么由金属代谢紊乱引起，要么由金属依赖的酶功能障碍引起。为了提高对特定疾病机制的认识或确定金属依赖酶的功能，能够可靠地确定元素浓度是至关重要的。只有电感耦合等离子体质谱仪器才能提供成功进行此类测量所需的高灵敏度和高质量精度。

DESCRIPTION (provided by applicant): In this application, we are requesting an ICP-MS (Inductively Coupled Plasma Mass Spectrometer) for the Metal Ion Core in the Department of Biochemistry and Molecular Biology at OHSU. An ICP-MS determines the concentration for elements from Li to U with high sensitivity, high mass accuracy and a broad linear detection range for elemental concentration. Because of the high mass accuracy and sensitivity experiments that determine isotope ratios are also possible. Due to these unique properties an ICP- MS is the perfect tool for a variety of biomedical projects that are typically performed at a medical research institution. Being able to reliably determine elemental concentrations in biological material is often crucial in disease related research projects especially if these diseases are caused or involve disturbances in metal metabolism. Accurate measurements of elemental content are also critical also when elucidating the function of metal containing enzymes or performing structural studies on proteins. Over the past 5 years the Metal Ion Core at OHSU has provided services related to metal and elemental concentrations in biological material to multiple researchers. Presently, the available instrumentation to determine elemental concentrations is often a limiting step. An ICP-MS would enable existing and new users of the Metal Ion Core to incorporate necessary but currently unavailable measurements in their research. The specific instrument that we selected is the Elan DRCII from Perkin Elmer. Perkin Elmer has been, by far, the leading manufacturer of ICP-MS instruments for the past 25 years. While instruments from other manufacturers are available they currently have only a very limited footprint in medical school settings like OHSU, where a plethora of different sample materials are typically analyzed. The Elan DRCII offers the best detection limits currently available while effectively eliminating interferences as they typically occur in biological material. Currently, there is no ICP-MS instrument on campus. Available instruments that are capable of determining metal concentrations on campus are limited by the range of linearity, inability to simultaneously measure multiple elements, or their requirements for large sample volumes. However, there is a clear need for an ICP-MS throughout OHSU. Researchers from several schools and departments within the school expressed their interest and strong support for acquiring an ICP-MS. PUBLIC HEALTH RELEVANCE A variety of severe disorders are either caused by disturbances in metal metabolism or by the dysfunction of metal dependent enzymes. To advance the knowledge about specific disease mechanisms or determine the function of metal dependent enzymes it is crucial to be able to reliably determine elemental concentrations. Only ICP-MS instrumentation offers the necessary high sensitivity and high mass accuracy to successfully perform such measurements.