ICP-MS to Measure Elemental Concentrations

描述（由申请人提供）：在本申请中，我们要求OHSU生物化学和分子生物学系的金属离子核心使用ICP-MS（电感耦合等离子体质谱仪）。ICP-MS以高灵敏度、高质量准确度和宽的元素浓度线性检测范围测定从Li到U的元素浓度。由于高质量精度和灵敏度，确定同位素比的实验也是可能的。由于这些独特的性质，ICP- MS是通常在医学研究机构进行的各种生物医学项目的完美工具。能够可靠地确定生物材料中的元素浓度在疾病相关的研究项目中通常是至关重要的，特别是如果这些疾病是由金属代谢引起或涉及金属代谢紊乱。当阐明含金属酶的功能或对蛋白质进行结构研究时，元素含量的准确测量也是至关重要的。在过去的5年中，OHSU的金属离子中心为多名研究人员提供了与生物材料中金属和元素浓度相关的服务。目前，测定元素浓度的可用仪器通常是一个限制性步骤。ICP-MS将使金属离子核心的现有和新用户能够在其研究中纳入必要但目前无法获得的测量。我们选择的特定仪器是Perkin Elmer的Elan DRCII。Perkin Elmer在过去的25年里一直是ICP-MS仪器的领先制造商。虽然来自其他制造商的仪器是可用的，但它们目前在OHSU等医学院环境中的足迹非常有限，在那里通常要分析过多的不同样品材料。Elan DRCII提供了目前最佳的检测限，同时有效地消除了生物材料中通常存在的干扰。目前，校园内没有ICP-MS仪器。能够在校园内测定金属浓度的现有仪器受到线性范围的限制，无法同时测量多种元素，或者它们对大样品体积的要求。然而，在整个OHSU显然需要ICP-MS。来自学校内的几个学校和部门的研究人员表达了他们对获得ICP-MS的兴趣和强烈支持。公共卫生相关性各种严重的疾病是由金属代谢紊乱或金属依赖酶功能障碍引起的。为了提高对特定疾病机制的认识或确定金属依赖性酶的功能，能够可靠地确定元素浓度至关重要。只有ICP-MS仪器才能提供成功执行此类测量所需的高灵敏度和高质量准确度。

DESCRIPTION (provided by applicant): In this application, we are requesting an ICP-MS (Inductively Coupled Plasma Mass Spectrometer) for the Metal Ion Core in the Department of Biochemistry and Molecular Biology at OHSU. An ICP-MS determines the concentration for elements from Li to U with high sensitivity, high mass accuracy and a broad linear detection range for elemental concentration. Because of the high mass accuracy and sensitivity experiments that determine isotope ratios are also possible. Due to these unique properties an ICP- MS is the perfect tool for a variety of biomedical projects that are typically performed at a medical research institution. Being able to reliably determine elemental concentrations in biological material is often crucial in disease related research projects especially if these diseases are caused or involve disturbances in metal metabolism. Accurate measurements of elemental content are also critical also when elucidating the function of metal containing enzymes or performing structural studies on proteins. Over the past 5 years the Metal Ion Core at OHSU has provided services related to metal and elemental concentrations in biological material to multiple researchers. Presently, the available instrumentation to determine elemental concentrations is often a limiting step. An ICP-MS would enable existing and new users of the Metal Ion Core to incorporate necessary but currently unavailable measurements in their research. The specific instrument that we selected is the Elan DRCII from Perkin Elmer. Perkin Elmer has been, by far, the leading manufacturer of ICP-MS instruments for the past 25 years. While instruments from other manufacturers are available they currently have only a very limited footprint in medical school settings like OHSU, where a plethora of different sample materials are typically analyzed. The Elan DRCII offers the best detection limits currently available while effectively eliminating interferences as they typically occur in biological material. Currently, there is no ICP-MS instrument on campus. Available instruments that are capable of determining metal concentrations on campus are limited by the range of linearity, inability to simultaneously measure multiple elements, or their requirements for large sample volumes. However, there is a clear need for an ICP-MS throughout OHSU. Researchers from several schools and departments within the school expressed their interest and strong support for acquiring an ICP-MS. PUBLIC HEALTH RELEVANCE A variety of severe disorders are either caused by disturbances in metal metabolism or by the dysfunction of metal dependent enzymes. To advance the knowledge about specific disease mechanisms or determine the function of metal dependent enzymes it is crucial to be able to reliably determine elemental concentrations. Only ICP-MS instrumentation offers the necessary high sensitivity and high mass accuracy to successfully perform such measurements.