Snapshot Image Mapping Spectrometer (IMS) for High Resolution Biological Imaging

用于高分辨率生物成像的快照图像映射光谱仪 (IMS)

• 批准号: 8325548
• 负责人: Robert Kester
• 金额: $ 34.32万
• 依托单位: REBELLION PHOTONICS, INC.
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8325548
• 关键词: Address; Americas; Area; Biological; Businesses; Calibration; Cells; Collection; Communities; Complex; Computer software; Custom; Data; Data Analyses; Data Collection; Development; Devices; Diamond; Elements; Event; Feedback; Fluorescent Probes; Four-dimensional; Funding Mechanisms; Future; Generations; Goals; Hour; Image; Imagery; Imaging Device; Investigation; Lead; Life; Light Microscope; Lighting; Location; Maps; Marketing; Methods; Microscope; Microscopy; Modeling; Noise; Optics; Paper; Performance; Phase; Photobleaching; Phototoxicity; Prize; Procedures; Process; Research; Research Personnel; Resolution; Rice; Sampling; Scanning; Scientist; Side; Signal Transduction; Small Business Innovation Research Grant; Societies; Software Tools; Solutions; Specimen; Speed; Structure; System; Techniques; Technology; Testing; Thailand; Time; Universities; Validation; Work; base; biological systems; cellular imaging; charge coupled device camera; commercialization; cost; design; detector; heart imaging; improved; innovation; insight; instrument; instrumentation; meetings; news; photonics; prototype; research study; sensor

DESCRIPTION (provided by applicant): Snapshot Image Mapping Spectrometer (IMS) for High Resolution Biological Imaging Indirect Imaging is proposing, through the SBIR funding mechanism, to develop an innovative imaging device that will allow economical snapshot hyperspectral imaging for real time microscopy and other biomedical applications, and is compatible with most research grade light microscopes. Recent advances in fluorescent probes, detector technology and micro-fabrication now make it possible to build an Image Mapping Spectrometer (IMS) - a device for rapid, real time quantitative spectral imaging. The IMS is a widefield method for acquiring full spectral information simultaneously from every pixel. It has superior signal-to-noise ratio compared to scanning hyperspectral systems and can be used with optical sectioning methods such as Nipkow disk. The IMS works by spatially redirecting image zones to obtain space between lines and using a multi-prism element to acquire simultaneously spectral and spatial information about the object. The final spectral cube is reconstructed by remapping the pixel locations from the CCD 2D image sensor to respective voxels (x, y, ;). This is a Phase I proposal, in which we will focus on (1) developing a larger format IMS system capable of collection a (x, y, ;) datacube of size 500 x 500 x 48 with an initial wavelength range of 450 to 700 nm and testing the Image Mapping Spectrometer against currently available spectral imaging systems in several live cell imaging applications. In parallel the project will pursue (2) developing the means to manufacture an Image Mapper at minimal costs - the fabrication process is currently expensive and time consuming taking 100+ hours/per part depending on the size and complexity. We will pursue a new diamond ruling fabrication approach that has a potential to dramatically shorten the fabrication time. In addition we will implement (3) automatic calibration procedures and software for real-time data analysis and visualization leading to optimized performance, improved resolution and frame-rate spectral unmixing capability. For the first time this will provide researchers with immediate, live feedback in real-time living cell hyperspectral imaging. In summary, the IMS has the potential to significantly advance a wide range of applications in the area of cellular imaging by reducing the phototoxicity and photobleaching and allowing hyperspectral analysis at high frame rates. To further its impact, in the future, we plan to combine the IMS with optical sectioning by using structured illumination, Nipkow disk confocal, and/or spatial deconvolution. These 4-dimensional imaging systems (X, Y, Z, ;) would further improve the signal-to-noise ratio of the collected images and improve their speed.

描述（由申请人提供）：用于高分辨率生物成像间接成像的快照图像映射光谱仪（IMS）建议通过SBIR资助机制开发一种创新的成像设备，该设备将允许经济的快照高光谱成像用于真实的时间显微镜和其他生物医学应用，并且与大多数研究级光学显微镜兼容。荧光探针、检测器技术和微加工的最新进展现在使得构建图像映射光谱仪（IMS）成为可能--一种用于快速、真实的时间定量光谱成像的设备。IMS是一种同时从每个像素获取全光谱信息的宽视场方法。与扫描高光谱系统相比，它具有上级信噪比，可与光学切片方法（如Nipkow盘）一起使用。IMS的工作原理是通过空间重定向图像区域以获得线之间的空间，并使用多棱镜元件来同时获取关于物体的光谱和空间信息。通过将来自CCD 2D图像传感器的像素位置重新映射到相应的体素（x，y，i）来重建最终的光谱立方体。 这是第一阶段提案，其中我们将专注于（1）开发更大格式的IMS系统，该系统能够收集尺寸为500 x 500 x 48的（x，y，;）数据立方体，初始波长范围为450至700 nm，并在几种活细胞成像应用中针对当前可用的光谱成像系统测试图像映射光谱仪。与此同时，该项目将致力于（2）开发以最低成本制造图像映射器的方法-制造过程目前昂贵且耗时，根据尺寸和复杂性，每个部件需要100多个小时。我们将寻求一种新的钻石统治制造方法，有可能大大缩短制造时间。此外，我们将实施（3）自动校准程序和软件，用于实时数据分析和可视化，从而优化性能，提高分辨率和帧率频谱分解能力。这将首次为研究人员提供实时活细胞高光谱成像的即时实时反馈。 总之，IMS具有通过减少光毒性和光漂白并允许在高帧速率下进行高光谱分析来显著推进细胞成像领域中的广泛应用的潜力。为了进一步发挥其影响，在未来，我们计划将IMS与光学切片相结合，通过使用结构化照明，Nipkow盘共焦，和/或空间解卷积。这些4维成像系统（X，Y，Z，i）将进一步提高所收集的图像的信噪比并提高它们的速度。