Molecular Screening Shared Resouce

分子筛选共享资源：人类基因组序列的完成， 老鼠、斑马鱼和其他一些模式生物已经初步识别了所有编码的 这些物种中的蛋白质。化学文库和全基因组文库的增殖 许多物种的抑制性RNA序列使寻找抑制性RNA序列成为可能 所有基因编码靶标的小分子和抑制性RNA。随之而来的发展 高通量筛选(NTS)技术使移动成为可能--借助正确的资源 这从概念到实践。加州大学洛杉矶分校JCCC分子筛选共享资源(MSSR)是 成立于2003年，以满足这一令人兴奋的科学目标。MSSR现在在 新加州纳米系统研究所，小分子文库，包含超过70,000个化合物，全基因组 酵母的敲除文库，以及鼠和人靶标的干扰RNA文库。太棒了 学术和技术领导层建立了一个强大的、可访问的筛查中心，帮助 开发可靠和可靠的酶和细胞培养HTS分析方法，并促进全基因组 屏幕。大卫·格芬医学院、加州纳米系统研究所、加州大学洛杉矶分校 文学与科学学院、几个单独的院系和一些私人捐赠者加入了 JCCC为设备和图书馆提供资金(远远超过1,000,000美元)和运营“启动” 需要启动并运行MSSR。共享资源已经运行(或当前正在运行)65 屏幕，其中绝大多数是为JCCC成员提供的。基因调控的成员，信号 转导与治疗学、肿瘤免疫学、癌细胞生物学、恶性血液病 分子流行病学与癌症发生、胸科肿瘤学和泌尿生殖科学发展计划 肿瘤学计划领域已经利用了这一共享资源。CCSG为此提出的预算请求 CCSG第一年的共享资源预算为估计运营预算的13%；支持 共享资源还来自美国国立卫生研究院辐射防护器中心CNSI，加州大学洛杉矶分校校长的 生物科学倡议、用户充值和慈善事业。(另请参阅关于共享的第6.2.3节 历史上的资源、描述、基本特征)。 代表四个癌症中心计划地区的八名癌症中心成员在 本报告所述期间的分子筛查共享资源；许多筛查正在进行中。 这是一个持续共享的资源。

The Molecular Screening Shared Resource (MSSR): Completion of the genome sequences of humans, mice, zebrafish and a number of other model organisms has led to the tentative identification of all encoded proteins in these species. The proliferation both of chemical libraries and of libraries of genome wide inhibitory RNA sequences for a number of species has made it possible to conceive of looking for inhibitory small molecules and inhibitory RNAs for all genetically encoded targets. The concomitant development of High Throughput Screening (NTS) technologies has made it possible - with the right resources - to move this from concept to practice. The UCLA JCCC Molecular Screening Shared Resource (MSSR) was established in 2003 to meet this exciting scientific objective. The MSSR now has dedicated space in the new California NanoSystems Institute, small molecule libraries containing over 70,000 compounds, genomewide knockout libraries for yeast, and interfering RNA libraries for both murine and human targets. Excellent academic and technical leadership has established a robust, accessible screening center that assists both in developing reliable and robust enzymatic and cell culture HTS assays and in facilitating genome-wide screens. The David Geffen School of Medicine, the California NanoSystems Institute (CNSI), the UCLA College of Letters and Science, several individual departments and some private donors have joined the JCCC to provide funding for the equipment and libraries (well over $1,000,000) and operating "start-up" required to get the MSSR up and running. The Shared Resource has run (or is currently running) 65 screens, the great majority of which are for JCCC members. Members of the Gene Regulation, Signal Transduction and Therapeutics, Tumor Immunology, Cancer Cell Biology, Hematopoietic Malignancies, Developing Program in Molecular Epidemiology and Carcinogenesis, Thoracic Oncology and Genitourinary Oncology Program Areas have already utilized this Shared Resource. The CCSG budget request for this Shared Resource for the first year CCSG budget is for 13% of the estimated operating budget; support for the shared resource also comes from the CNSI, an NIH Center for Radioprotectors, the UCLA Chancellor's Biosciences Initiative, user recharges and philanthropy. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). Eight Cancer Center members representing four Cancer Center Program Areas completed screens in the Molecular Screening Shared Resource during the reporting period; many screens are ongoing. This is a continuing shared resource.