STRUCTURE ACTIVITY RELATIONSHIPS OF TRPM7 ION CHANNEL INHIBITORS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 由于我作为夏威夷太平洋大学共享仪器INBRE核心的领导者和初级调查员的双重角色，我不得不平衡两套责任。就背景而言，首先必须指出的是，由于分包合同的问题，HPU直到2010年12月底才收到资金。不幸的是，这所大学目前没有预付资金的组织机制，因此，在某种程度上，我们的开始被推迟了。然而，随着一个新的校园内BL2生物分析实验室的建设完成，并将于本周开始进行分析，我将能够将更多的时间分配到我的外喜菌素A类似物项目中。由于HPU没有为新聘用的教师提供“启动”资金的一致政策，INBRE II的资金在为我开始独立研究生涯所需的设备和用品方面发挥了无价的作用。因此，还花费了大量时间研究各种材料和用品资源。三名年轻的研究人员最近加入了该项目，他们的培训已经开始。在这项培训工作中，我还开始制作一系列培训视频，教授特定于天然产品合成的实验室技能和技术。我们还收集了少量的天然外新素A用于衍生化，因此，最初的任务之一是开始努力收集其来源--软珊瑚Sarcothelia edmondsoni，以及随后提纯外生素A。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Due to my dual roles as the leader of the Hawaii Pacific University Shared Instrumentation INBRE Core and that as a junior investigator, I have had to balance two sets of responsibilities. For context, it is first important to note that due to issues with the sub-contract, HPU did not receive funds until late December 2010. Unfortunately, the university presently has no organized mechanism for advanced funding and thus, to some degree, our start was delayed. However, with construction of a new on-campus BL2 bioassay lab complete and assays to begin this week, I will be able to allocate much more of my time into my waixenicin A analogue project. Because HPU has no consistent policy in place to provide new faculty hires with "start-up" funds the INBRE II funding has been invaluable in supplying me with the necessary equipment and supplies to start my independent research career. Thus, a significant amount of time has also been spent researching various resources of materials and supplies. Three young researchers have recently been brought on board to the project and their training has begun. In this training endeavor, I have also begun to produce a collection of training videos that teach laboratory skills and techniques specific to natural product synthesis. We are also collecting modest quantities of natural Waixencin A for derivatization and thus one of the very first tasks was to initiate efforts towards the collection of its source, soft coral Sarcothelia edmondsoni, as well as the subsequent purification of waixenicin A.