EFFECTS OF TESTOSTERONE & SOCIAL INTERACTIONS ON MEMORY & ADULT NEUROGENESIS

睾酮的作用

• 批准号: 8360427
• 负责人: Mark D. Spritzer
• 金额: $ 8.1万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360427
• 关键词: Adult; Brain; Bromodeoxyuridine; Cell Survival; Cells; Funding; Genetic; Grant; Hippocampus (Brain); Label; Learning; Memory; National Center for Research Resources; Neurons; Oils; Performance; Principal Investigator; Proliferating; Radial; Rattus; Research; Research Infrastructure; Resources; Running; Short-Term Memory; Social Interaction; Source; Staining method; Stains; Testing; Testosterone; Testosterone Propionate; United States National Institutes of Health; Vermont; Work; adult neurogenesis; arm; cost; improved; male; neurogenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Previous research indicates that testosterone enhances the survival of newly proliferated neurons in the adult hippocampus, and increased survival of hippocampal neurons may correlate with improved spatial memory. Therefore, adult neurogenesis may be the mechanism by which testosterone improves spatial working memory. We examined both performance on a working-reference memory version of the 8-arm radial maze (RAM) and 30-day hippocampal cell survival using castrated adult male rats. Subjects were injected with BrdU (200 mg/kg) to assess the survival of newly proliferated cells within the hippocampus. Beginning the following day, rats were run once daily for 29 days on the RAM. The number of both working memory errors (WME) and reference memory errors (RME) was scored. Each day prior to maze testing, rats were injected with either 0.5 mg of testosterone propionate (n = 10) or 0.1ml of oil vehicle (n = 10). BrdU-labeled cells were stained and scored on brain sections. Testosterone-injected subjects performed significantly fewer WMEs than did controls during the first ten days of testing. In contrast, testosterone had no significant effect on RMEs. This indicates that testosterone improves working memory but not reference memory. Surprisingly, testosterone had no effect on cell survival. This may have been because learning on the maze elevated neurogenesis levels in the control subjects. These results suggest that enhanced hippocampal cell survival may not be the mechanism by which testosterone improves spatial memory.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 先前的研究表明，睾酮增强成年海马中新增殖的神经元的存活，并且海马神经元存活的增加可能与空间记忆的改善相关。因此，成年神经发生可能是睾酮改善空间工作记忆的机制。我们研究了这两个性能的工作参考记忆版本的8臂径向迷宫（RAM）和30天的海马细胞存活率使用去势成年雄性大鼠。受试者注射BrdU（200 mg/kg），以评估海马内新增殖细胞的存活率。从第二天开始，大鼠在RAM上每天跑一次，持续29天。对工作记忆错误（WME）和参考记忆错误（RME）的数量进行评分。每天在迷宫测试之前，向大鼠注射0.5mg丙酸睾酮（n = 10）或0.1ml油载体（n = 10）。对BrdU标记的细胞进行染色并在脑切片上评分。在测试的前十天，注射睾酮的受试者的WME明显少于对照组。相比之下，睾酮对RME没有显着影响。这表明睾酮改善了工作记忆，但没有改善参考记忆。令人惊讶的是，睾酮对细胞存活没有影响。这可能是因为在迷宫中学习提高了对照受试者的神经发生水平。这些结果表明，增强海马细胞存活可能不是睾酮改善空间记忆的机制。