The Role of Nag-1 in colon cancer and aging

Nag-1 在结肠癌和衰老中的作用

• 批准号: 8535333
• 负责人: KELLY INMAN
• 金额: $ 22.56万
• 依托单位: INTEGRATED LABORATORY SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2013-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8535333
• 关键词: Aging; Animals; Carcinogens; Colon Carcinoma; Diet; Dose; Fatty acid glycerol esters; Female; GDF15 gene; Gene Expression; Intraperitoneal Injections; Knock-out; Leptin; Longevity; Malignant Neoplasms; Mus; Obesity; Resistance; Role; Serum; Time; Wild Type Mouse; feeding; tumor

To determine whether the NAG-Tg mice are resistant to high-fat-diet-induced obesity followed by cancer resistant, mice (NAG-Tg, knockouts and control littermates) will be fed regular chow or a high-fat diet. NAG-Tg, KO, and control mice (6-8 week old) will be treated at weekly intervals with six doses of AOM (10 mg/kg) by intraperitoneal injection. In addition, the purpose of this study is to evaluate the impact of a high fat diet on life span, serum leptin and NAG-1 levels, and gene expression in female Nag-1 mice. Wild type and NAG-1 females from lines 1377 and 1398 are being feed a diet of 10% or 60% fat.

为了确定 NAG-Tg 小鼠是否能够抵抗高脂肪饮食诱导的肥胖，进而抵抗癌症，将给小鼠（NAG-Tg、基因敲除小鼠和对照同窝小鼠）喂食普通食物或高脂肪饮食。 NAG-Tg、KO 和对照小鼠（6-8 周龄）将每周通过腹膜内注射六剂 AOM（10 mg/kg）进行治疗。此外，本研究的目的是评估高脂肪饮食对雌性 Nag-1 小鼠寿命、血清瘦素和 NAG-1 水平以及基因表达的影响。来自品系 1377 和 1398 的野生型和 NAG-1 雌性正在饲喂 10% 或 60% 脂肪的饮食。