Rapid breath detection of colorectal cancer with a colorimetric sensor array

描述（由申请人提供）：项目概要/摘要最近，据报道犬科动物可以从呼出气的气味中检测结肠癌的存在，具有91%的选择性和99%的特异性（Sonoda，H.例如，Gut，2011，60，814）。能够执行类似操作的设备的前景打开了快速、非侵入性、准确和廉价的结肠癌呼吸测试的前景。iSense Medical Corp.（IMC）传感器的操作类似于生物嗅觉，因为不同阵列的交叉反应化学传感器被呼吸样本激活。先前的工作已经表明，基于嵌入在非常高表面积的纳米多孔溶胶-凝胶基质中的比色指示剂的这种传感器系统能够区分复杂的混合物（Suslick，B.例如，Anal. 2010，82，2067），并且最近在克利夫兰临床基金会（CCF）的临床测试中显示，以85%的准确度跨阶段从呼出气检测肺癌的存在（Mazzone，P. J. Thoracic Oncology，出版中）。根据该合同，我们建议定制纳米多孔指示剂阵列，以优化其对活性结肠肿瘤小分子代谢物的灵敏度，并在第二阶段在CCF进行试点研究，以量化呼吸中结肠癌检测的准确性。 公共卫生相关性：项目叙述拟议的研究工作将开发一种快速，廉价和非侵入性的诊断测试结肠癌的存在，作为后续结肠镜检查的提示。该技术的另一个应用是呼吸测试，以非侵入性和廉价地监测化疗治疗的疗效。

DESCRIPTION (provided by applicant): Project Summary/Abstract Recently it has been reported that canines can detect the presence of colon cancer from the scent of exhaled breath, with 91% selectivity and 99% specificity (Sonoda, H. et al., Gut, 2011, 60, 814). The prospect of a device which can perform similarly opens the prospect of a rapid, non-invasive, accurate and inexpensive breath test for colon cancer. The iSense Medical Corp. (IMC) sensor operates analogously to biological olfaction in that a diverse array of cross- reactive chemical sensors is activated by the breath sample. Prior work has shown this sensor system, based on colorimetric indicators embedded in a nanoporous sol-gel matrix of very high surface area, to be capable of discrimination of complex mixtures (Suslick, B. et al., Anal. Chem. 2010, 82, 2067), and has recently been shown in clinical testing at the Cleveland Clinic Foundation (CCF) to detect the presence of lung cancer from exhaled breath, across stages, with accuracy of 85% (Mazzone, P. et al., J. Thoracic Oncology, in press). Under this contract we propose to customize the nanoporous indicator array to optimize its sensitivity to small molecule metabolites of active colon tumor, and in Phase 2 conduct a pilot study at CCF to quantify accuracy of detection of colon cancer from breath, by stage. PUBLIC HEALTH RELEVANCE: Project Narrative The proposed research effort will develop a rapid, inexpensive and non-invasive diagnostic test for the presence of colon cancer, as a prompt for subsequent colonoscopic examination. A further application of this technology would be a breath test to non-invasively and inexpensively monitor the efficacy of chemotherapy treatment.