Oxidative Stress Biosensor for Breath-Based Environmental Monitoring

用于基于呼吸的环境监测的氧化应激生物传感器

• 批准号: 9408215
• 负责人: Ray Anthony Martino
• 金额: $ 14.99万
• 依托单位: ISENSE, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9408215
• 关键词: Acetone; Acute; Air Pollution; Aldehydes; Algorithms; Ammonia; Antioxidants; Area; Benchmarking; Biochemical; Biochemical Process; Biological Markers; Biosensor; Breath Tests; Cancer Patient; Carbon Monoxide; Cardiovascular Diseases; Chemicals; Chemistry; Chronic Disease; Classification; Clinic; Complex Mixtures; Data Analyses; Detection; Development; Devices; Disease; Distress; Endocrine Disruptors; Environmental Exposure; Environmental Monitoring; Evaluation; Exhalation; Failure; Fingerprint; Formulation; Health; Health Status; Heavy Metals; Human; Human Resources; Hydrocarbons; Hydrogen Peroxide; Hydrogen Sulfide; Individual; Industrialization; Inflammatory Response; Isoprene; Kidney Diseases; Libraries; Malignant neoplasm of lung; Malondialdehyde; Measurement; Measures; Methods; Monitor; Neurodegenerative Disorders; Nitric Oxide; Nitrogen Oxides; Noise; Organ; Oxidative Stress; Pathogenesis; Pathway interactions; Patient Care; Patients; Performance; Peroxides; Pesticides; Phase; Physical Examination; Physicians; Physiological; Production; Public Health; Reactive Oxygen Species; Reporting; Reproducibility; Research; Screening Result; Sensitivity and Specificity; Signal Transduction; System; Technology; Testing; Time; Toxic Environmental Substances; Vulnerable Populations; Work; Xenobiotics; base; biomarker identification; biosignature; carcinogenesis; chemical fingerprinting; environmental stressor; hexanoic acid; improved; insight; interest; n-hexanal; novel; optical sensor; oxidation; oxidative damage; portability; repaired; response; sensor; success; tool; urban area

PROJECT SUMMARY/ABSTRACT This application seeks to develop a non-invasive and practical optical sensor technology to monitor and quantify oxidative health. Oxidative stress (OS) is a biochemical imbalance in which production of reactive oxygen species exceeds the system’s antioxidant capacity. Failure to repair or contain cumulative oxidative damage is likely responsible for the pathogenesis of many acute and chronic illnesses, including cardiovascular and kidney disease, inflammatory responses, neurodegenerative disorders, and carcinogenesis. A noninvasive, inexpensive, robust sensor capable of evaluating and monitoring systemic oxidative stress would be a tool of great value to public health officials, clinicians, and individual consumers since many environmental conditions and xenobiotics (e.g., urban air pollution, heavy metal contamination, endocrine disrupting compounds, pesticides, and industrial chemicals) trigger oxidative stress. Because there is no universal biomarker for OS and there are many potential target molecules, it’s critical to develop a system that is capable of detecting many chemical components and creating a chemical fingerprint that reflects alterations in key pathways associated with environmental stressors. iSense, LLC has developed a novel sensor array that can detect a vast library of volatile compounds across multiple chemical moieties at ppb-level concentrations. The technology is rapid, inexpensive, and capable of distinguishing between single analytes and complex mixtures. Our breath sensor has been used to detect the biosignature of lung cancer patients and differentiate between different types of lung cancer in humans. Phase I of this project will test, improve, and optimize this breath analysis technology towards the detection and identification of key biomarkers of oxidative stress. Successful completion of this work will establish the fundamental chemistry necessary to develop a device to measure and understand markers of environmental exposure.

项目摘要/摘要 该应用程序旨在开发一种非侵入性和实用的光学传感器技术来监视和 量化氧化健康。氧化应激（OS）是生化不平衡，其中反应性产生 氧气超过了系统的抗氧化能力。无法修复或含有累积氧化物 损害可能导致许多急性和慢性病的发病机理，包括心血管 和肾脏疾病，炎症反应，神经退行性疾病和癌变。无创的， 廉价，稳健的传感器能​​够评估和监测系统性氧化应激将是一种工具 由于许多环境，对公共卫生官员，临床医生和个人消费者的重视 疾病和异生元（例如，城市空气污染，重金属污染，内分泌干扰 化合物，农药和工业化学物质）触发氧化物胁迫。因为没有通用 OS的生物标志物，并且有许多潜在的靶标分子，开发一个能力的系统至关重要 检测许多化学成分并创建一个化学指纹，以反映钥匙的变化 与环境压力源相关的途径。 Isense，LLC开发了一个新型的传感器阵列，可以 在PPB级浓度下检测多个化学部分的巨大挥发性化合物库。这 技术快速，便宜，能够区分单个分析物和复杂混合物。 我们的呼吸传感器已用于检测肺癌患者的生物签名并区分 人类的不同类型的肺癌。该项目的第一阶段将测试，改进和优化这种呼吸 分析技术用于检测和鉴定氧化应激的关键生物标志物。成功的 这项工作的完成将确定开发一种衡量设备所必需的基本化学反应 了解环境暴露的标记。