Oxidative Stress Biosensor for Breath-Based Environmental Monitoring

用于基于呼吸的环境监测的氧化应激生物传感器

• 批准号: 9408215
• 负责人: Ray Anthony Martino
• 金额: $ 14.99万
• 依托单位: ISENSE, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9408215
• 关键词: Acetone; Acute; Air Pollution; Aldehydes; Algorithms; Ammonia; Antioxidants; Area; Benchmarking; Biochemical; Biochemical Process; Biological Markers; Biosensor; Breath Tests; Cancer Patient; Carbon Monoxide; Cardiovascular Diseases; Chemicals; Chemistry; Chronic Disease; Classification; Clinic; Complex Mixtures; Data Analyses; Detection; Development; Devices; Disease; Distress; Endocrine Disruptors; Environmental Exposure; Environmental Monitoring; Evaluation; Exhalation; Failure; Fingerprint; Formulation; Health; Health Status; Heavy Metals; Human; Human Resources; Hydrocarbons; Hydrogen Peroxide; Hydrogen Sulfide; Individual; Industrialization; Inflammatory Response; Isoprene; Kidney Diseases; Libraries; Malignant neoplasm of lung; Malondialdehyde; Measurement; Measures; Methods; Monitor; Neurodegenerative Disorders; Nitric Oxide; Nitrogen Oxides; Noise; Organ; Oxidative Stress; Pathogenesis; Pathway interactions; Patient Care; Patients; Performance; Peroxides; Pesticides; Phase; Physical Examination; Physicians; Physiological; Production; Public Health; Reactive Oxygen Species; Reporting; Reproducibility; Research; Screening Result; Sensitivity and Specificity; Signal Transduction; System; Technology; Testing; Time; Toxic Environmental Substances; Vulnerable Populations; Work; Xenobiotics; base; biomarker identification; biosignature; carcinogenesis; chemical fingerprinting; environmental stressor; hexanoic acid; improved; insight; interest; n-hexanal; novel; optical sensor; oxidation; oxidative damage; portability; repaired; response; sensor; success; tool; urban area

PROJECT SUMMARY/ABSTRACT This application seeks to develop a non-invasive and practical optical sensor technology to monitor and quantify oxidative health. Oxidative stress (OS) is a biochemical imbalance in which production of reactive oxygen species exceeds the system’s antioxidant capacity. Failure to repair or contain cumulative oxidative damage is likely responsible for the pathogenesis of many acute and chronic illnesses, including cardiovascular and kidney disease, inflammatory responses, neurodegenerative disorders, and carcinogenesis. A noninvasive, inexpensive, robust sensor capable of evaluating and monitoring systemic oxidative stress would be a tool of great value to public health officials, clinicians, and individual consumers since many environmental conditions and xenobiotics (e.g., urban air pollution, heavy metal contamination, endocrine disrupting compounds, pesticides, and industrial chemicals) trigger oxidative stress. Because there is no universal biomarker for OS and there are many potential target molecules, it’s critical to develop a system that is capable of detecting many chemical components and creating a chemical fingerprint that reflects alterations in key pathways associated with environmental stressors. iSense, LLC has developed a novel sensor array that can detect a vast library of volatile compounds across multiple chemical moieties at ppb-level concentrations. The technology is rapid, inexpensive, and capable of distinguishing between single analytes and complex mixtures. Our breath sensor has been used to detect the biosignature of lung cancer patients and differentiate between different types of lung cancer in humans. Phase I of this project will test, improve, and optimize this breath analysis technology towards the detection and identification of key biomarkers of oxidative stress. Successful completion of this work will establish the fundamental chemistry necessary to develop a device to measure and understand markers of environmental exposure.

项目摘要/摘要 这一应用寻求开发一种非侵入性和实用的光学传感器技术来监测和 量化氧化健康。氧化应激(OS)是一种生化失衡，在这种失衡中， 氧气的种类超过了系统的抗氧化能力。未能修复或遏制累积氧化 损伤可能是许多急慢性疾病的致病原因，包括心血管疾病。 肾脏疾病、炎症反应、神经退行性疾病和癌症。一种非侵入性， 能够评估和监测全身氧化应激的廉价、坚固的传感器将是一种工具 对公共卫生官员、临床医生和个人消费者具有极大的价值，因为许多环境 环境和异生物质(例如，城市空气污染、重金属污染、内分泌干扰 化合物、杀虫剂和工业化学品)会引发氧化应激。因为没有万能的 OS的生物标记物和许多潜在的靶分子，开发一种能够 检测许多化学成分并创建反映密钥变化的化学指纹 与环境应激源相关的通路。ISense，LLC开发了一种新型传感器阵列，可以 检测ppb级别的多个化学部分的大量挥发性化合物。这个 这项技术快速、廉价，能够区分单一分析物和复杂混合物。 我们的呼吸传感器已被用于检测肺癌患者的生物特征，并区分 人类患上不同类型的肺癌。该项目的第一阶段将测试、改进和优化这一呼吸 氧化应激的关键生物标志物的检测和识别的分析技术。成功 这项工作的完成将建立必要的基础化学，以开发一种测量和 了解环境暴露的标志。