Laboratory Testing Core

实验室测试核心

• 批准号: 8247047
• 负责人: Daniel A Lawrence
• 金额: $ 22.77万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247047
• 关键词: Anticoagulants; Antigens; Atherosclerosis; Biological Assay; Bleeding time procedure; Blood Platelets; Chemicals; Coagulation Process; Development; Ensure; Fibrin fragment D; Fibrinolysis; Genomics; Goals; Immunoassay; Individual; Inflammatory; Laboratories; Libraries; Lipids; Measures; Mouse Protein; Mouse Strains; Mus; Obesity; Plasma; Plasminogen Activator; Plasminogen Activator Inhibitor 1; Platelet aggregation; Proteins; Research Personnel; Risk; Screening procedure; Technology; Testing; Thrombosis; Time; Venous Thrombosis; Zebrafish; adipokines; cytokine; hypercholesterolemia; novel; programs; small molecule; success

Core B: Laboratory Testing Core The Testing Laboratory Core B has the goal to serve every project in the Program. It will provide an array of assays assessing fibrinolysis, coagulation, anticoagulant activities, and plasma lipid profiles. The Core will also provide assays of inflammatory cytokines, and adipokines as well as with established kits for mouse proteins associated with thrombosis, atherosclerosis and obesity. The specific assays that are available for each of the projects are indicated below. Core B will also coordinate the high through-put screening of small molecules by the Center for Chemical Genomics. Project 1: Core B will perform analysis of plasma PAI-1 levels for both active and inactive PAI-1 and of plasminogen activator antigen and activity levels. In addition, plasma lipid profiles will be routinely determined and immunoassays of various inflammatory cytokines as well as adipokines will be measured in a mutiplex format using the Luminex Technology. High through-put screening of small molecule compound libraries for the development of novel PAI-1 inactivating agents will also be performed through the Center for Chemical Genomics. Project 2: Core B will perform analysis of plasma PAI-1 levels for both active and inactive PAI-1 and of plasminogen activator antigen and activity levels. Murine plasmas will also be analyzed for increased risk for thrombosis by determining the levels of plasma coagulation, and anticoagulant proteins in various strains of mice at baseline, and after venous thrombosis with or without hypercholesterolemia. D-dimer levels will also be measured. Procoagulant microparticles will also be measured as well as immunoassays of various inflammatory cytokines. Project 3: Core B will assist the investigators off Project 3 by assessing platelet function including bleeding times and platelet aggregation in mice. Fibrinolytic parameters will be determined including PAI-1 and plasminogen activator levels. Immunoassays of various inflammatory cytokines will also be measured in a mutiplex format using the Luminex Technology. Project 4: Core B will assist the investigators of Project 4 by developing novel, assays for coagulation/fibrinolysis and platelet function in Zebrafish. These assays will include immunoassays and functional assays such as clotting times as well as factor assays. The overall goals of the Testing Laboratory Core B are to enhance the success of each individual project by providing rigorous, standardized assays and to ensure that the combined Program is stronger than each of its individual parts.

核心B：实验室测试核心 测试实验室核心B的目标是为项目中的每个项目提供服务。它将提供一个 评估纤维蛋白溶解、凝血、抗凝活性和血浆脂质分布的测定。芯会 还提供了炎性细胞因子和脂肪因子的测定以及用于小鼠的已建立的试剂盒， 与血栓形成、动脉粥样硬化和肥胖症相关的蛋白质。可用于以下目的的特定检测试剂盒 每个项目如下。核心B也将协调小的高通量筛选 化学基因组学中心（Center for Chemical Genomics） 项目1：核心B将分析活性和非活性派-1的血浆派-1水平， 纤溶酶原激活物抗原和活性水平。此外，还将常规检测血脂谱。 测定和免疫测定各种炎性细胞因子以及脂肪因子， 使用Luminex技术的多路复用格式。小分子化合物的高通量筛选 用于开发新型派-1灭活剂的文库也将通过 化学基因组学 项目2：核心B将分析活性和非活性派-1的血浆派-1水平以及 纤溶酶原激活物抗原和活性水平。还将分析鼠血浆中的以下风险增加： 血栓形成通过测定血浆凝固水平，和抗凝蛋白在各种菌株， 在基线和静脉血栓形成后，有或没有高胆固醇血症的小鼠。D-二聚体水平也将 被衡量。还将测量促凝血剂微粒以及各种促凝血剂的免疫测定。 炎性细胞因子 项目3：核心B将通过评估血小板功能（包括出血）协助研究者退出项目3 时间和小鼠血小板聚集。将测定纤溶参数，包括派-1和 纤溶酶原激活物水平。各种炎性细胞因子的免疫测定也将在免疫组织化学中测量。 使用Luminex技术的多路复用格式。 项目4：核心B将协助项目4的研究者开发新的检测方法， 凝血/纤维蛋白溶解和血小板功能。这些测定将包括免疫测定和 功能测定如凝血时间以及因子测定。 测试实验室核心B的总体目标是通过以下方式提高每个项目的成功率 提供严格、标准化的检测，并确保合并后的计划比 它的各个部分。