Dissection of the TORC1 Signaling Network in Yeast

酵母中 TORC1 信号网络的剖析

• 批准号: 8334661
• 负责人: Andrew Paul Capaldi
• 金额: $ 27.65万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-30 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8334661
• 关键词: Accounting; Address; Aging; Animal Model; Biochemical; Biological Models; Biological Process; Cells; Cellular Stress; Complex; Computing Methodologies; DNA Microarray Chip; Data; Diabetes Mellitus; Disease; Dissection; Eukaryota; Eukaryotic Cell; Event; Gene Expression; Genes; Genomics; Goals; Growth; Hormones; Human; Inositol; Light; MAP Kinase Gene; MAPK14 gene; Malignant Neoplasms; Measurement; Measures; Metabolism; Methods; Microarray Analysis; Modeling; Neoplasm Metastasis; Nutrient; Obesity; Output; Pathway interactions; Phosphorylation; Phosphotransferases; Post-Translational Protein Processing; Protein Biosynthesis; Proteins; Regulation; Repression; Research; Ribosomes; Saccharomyces cerevisiae; Second Messenger Systems; Series; Signal Pathway; Signal Transduction; Signaling Protein; Sirolimus; Stimulus; Stress; Structure; Study models; System; Work; Yeasts; base; cancer cell; cell growth; chemotherapy; data modeling; deprivation; genome-wide; high throughput screening; mutant; network models; protein protein interaction; research study; second messenger

DESCRIPTION (provided by applicant): Eukaryotic cells utilize a complex network of signaling pathways to regulate cell growth. This network is known to control aging and its malfunction leads to a wide range of diseases. At the center of the network lies the target of rapamycin complex 1 (TORC1) pathway. In the presence of the appropriate hormones and nutrients, this pathway is active and drives growth by inducing ribosome and protein synthesis and repressing catabolic metabolism. However, when nutrient or hormone levels are low, or cells are exposed to noxious stress, TORC1 signaling is inactivated and growth is slowed. How TORC1 activity is regulated remains largely unknown, and in particular it is uncertain (1) what output the complex branched TOR pathway has in stress, (2) which stress signaling pathways are involved in TOR regulation, and (3) how they impinge on the TOR pathway itself. Here we propose to address these questions in the model organism S. cerevisiae, taking advantage of the fact that the structure and function of the TORC1 pathway is highly conserved from yeast to human. In preliminary studies, we have examined TORC1 signaling in osmotic stress and find that stress alters TORC1 pathway output differently than other stimuli, such as nutrient deprivation and rapamycin. In addition, we find that TORC1 pathway repression depends significantly on the activity of the stress activated MAPK Hog1/p38. Building on these results, we will now construct a quantitative model of TOR pathway regulation and output in stress using a multipronged approach. First, we will use DNA microarray analysis of mutant strains to construct a model of the TOR/Hog1 signaling circuit to explain how stress and Hog1 alter signals transmitted through TORC1 and other growth pathways. We will then use high-throughput screens to identify additional upstream of regulators of TORC1, and then determine how these proteins interact with each other and the TOR pathway, again using microarray analysis. Finally we will add mechanistic detail to our model by studying the protein-protein interactions and post-translational modifications underlying the regulatory events we uncover. The proposed research will provide the first detailed view of the signaling pathways underlying stress dependent growth/TORC1 regulation in eukaryotes. This will not only shed light onto a fundamental and poorly understood regulatory system, but due to the high level of conservation in the TORC1 and stress pathways, our data and model will likely have a profound influence on our understanding of TOR and cell growth related diseases such as cancer, aging, obesity and diabetes.

描述（由申请人提供）：真核细胞利用复杂的信号通路网络来调节细胞生长。众所周知，这个网络可以控制衰老，它的功能失调会导致各种疾病。在网络的中心是雷帕霉素复合物1（TORC 1）途径的目标。在适当的激素和营养物质的存在下，这一途径是活跃的，并通过诱导核糖体和蛋白质合成和抑制分解代谢来驱动生长。然而，当营养或激素水平较低，或细胞暴露于有害应激时，TORC 1信号转导被灭活，生长减慢。TORC 1活性是如何调节的在很大程度上仍然是未知的，特别是不确定的是（1）复杂的分支TOR通路在应激中有什么输出，（2）哪些应激信号通路参与TOR调节，以及（3）它们如何影响TOR通路本身。在这里，我们建议在模式生物S中解决这些问题。利用TORC 1途径的结构和功能从酵母到人是高度保守的这一事实，在初步研究中，我们已经研究了渗透胁迫中的TORC 1信号传导，并发现胁迫改变TORC 1通路的输出不同于其他刺激，如营养剥夺和雷帕霉素。此外，我们发现，TORC 1通路的抑制显着依赖于应激激活的MAPK Hog 1/p38的活性。在这些结果的基础上，我们现在将使用多管齐下的方法构建压力下TOR通路调节和输出的定量模型。首先，我们将使用突变株的DNA微阵列分析来构建TOR/Hog 1信号通路的模型，以解释压力和Hog 1如何改变通过TORC 1和其他生长途径传递的信号。然后，我们将使用高通量筛选来识别TORC 1的其他上游调节因子，然后再次使用微阵列分析来确定这些蛋白质如何相互作用以及TOR通路。最后，我们将通过研究蛋白质-蛋白质相互作用和我们发现的调控事件的翻译后修饰，为我们的模型添加机制细节。这项研究将提供真核生物中压力依赖性生长/TORC 1调节的信号通路的第一个详细视图。这不仅将揭示一个基本的和不太了解的调控系统，而且由于TORC 1和应激途径的高度保守性，我们的数据和模型可能会对我们理解TOR和细胞生长相关疾病（如癌症，衰老，肥胖和糖尿病）产生深远的影响。