Animal Core

动物核心

• 批准号: 8379778
• 负责人: MICHELE T PRITCHARD
• 金额: $ 4.46万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8379778
• 关键词: Acute; Alcoholic Liver Diseases; Alcohols; Animal Model; Animals; Biochemical; Body Weight; Cell Separation; Cells; Chronic; Clinic; Collaborations; Complex; Control Animal; Cost Savings; Data; Databases; Deltastab; Diet; Eating; Endothelial Cells; Ensure; Equipment and supply inventories; Ethanol; Exposure to; Goals; Harvest; Hepatic; Hepatic Stellate Cell; Hepatocyte; Human Resources; In Vitro; Kupffer Cells; Laboratories; Modeling; Molecular; Mononuclear; Mus; Pathogenesis; Pilot Projects; Plasma; Primary Cell Cultures; Procedures; Process; Protocols documentation; Rattus; Research Infrastructure; Research Institute; Research Personnel; Rodent; Rodent Model; Sampling; Techniques; Testing; Tissue Sample; Tissues; Work; abstracting; alcohol exposure; alcohol response; animal resource; biomedical resource; experience; feeding; innovation; member; novel; operation

The Animal Model Core will be managed from the Department of Pathobiology, but will be active at both the Animal Resource Center (ARC) at CWRU and the Biomedical Resource Center (BRU) in the Lerner Research Institute at the Cleveland Clinic (CC). The functional operation of this core predates the submission of this proposal and, therefore, the proposed Animal Models and Cell Isolation Core (Animal Core) benefits from our experienced personnel and the existing infrastructure at both the BRU and ARC facilities. Dr. Nagy's laboratory has extensive experience with the use of the Lieber-DeCarli ad libitum ethanol exposure model in both rats and mice (1-4). In the past several years, Dr. Nagy's group has collaborated with each of the Pis on the Exploratory Projects to provide plasma, tissue and isolated cell samples from rats and mice after acute and chronic ethanol exposures. We have experience in coordinating the procedures required and have carried out these functions in both the BRU and ARC facilities. Recent abstracts from collaborations between members of the Cleveland Alcohol Center using samples from our pre-existing core are listed below in section E.

动物模型核心将由病理生物学系管理，但将在两个部门都活跃。 CWRU动物资源中心（ARC）和Lerner研究中心生物医学资源中心（BRU） 克利夫兰诊所（Cleveland Clinic）这一核心的功能运作早于本报告的提交。 因此，提出的动物模型和细胞分离核心（动物核心）受益于我们的 经验丰富的人员和BRU和ARC设施的现有基础设施。纳吉医生的 实验室在使用Lieber-DeCarli自由乙醇暴露模型方面具有丰富的经验， 大鼠和小鼠（1-4）。在过去的几年里，纳吉博士的团队与每一个Pis合作， 探索性项目，提供急性和慢性感染后大鼠和小鼠的血浆、组织和分离细胞样本， 慢性酒精暴露我们在协调所需程序方面有经验，并已执行 BRU和ARC设施中的这些功能。成员之间的合作的最新摘要 克利夫兰酒精中心使用我们现有的核心样品进行的研究列于下文E节。