TISSUE CORE FACILITY

项目总结（见说明）： 组织核心（TC）的目的是帮助促进同行评审的科学需求。癌症中心的研究人员通过提供集中的服务和技术集中在高质量，丰富的注释人类生物标本。技术委员会由四个不同的业务部门组成，提供生物标本收集、处理、注释、储存和最终释放所需的端到端生物储存服务。TC-摄入和采集部分负责收集和 生物样本的初始注释和去标识化，用于研究者驱动和一般银行方案。 TC样本处理实验室提供各种生物样本处理服务，从血液处理到核酸提取。TC研究组织学服务部门通过提供基本的组织学服务，研究重点免疫组织化学和组织微阵列的构建来支持CCSG研究人员。TC-终身癌症筛查作为该中心终身癌症筛查和预防中心的组织核心卫星设施运作。技术人员负责操作和维护核心仪器和技术。 在上一个赠款期间，核心建立了先进的，国家的科学技术，以支持免疫组织化学（Ventana发现自动化系统），自动化，高通量核酸提取系统，（Qiagen）和最近的共享仪器赠款资助的自动冷冻系统。还启动了一个新的生物样本库存管理系统（LabVantage生物库）。 新的LabVantage生物库系统提供集中的全面样本管理，同意验证和生物标本去识别，使核心有效地作为一个机构诚实的经纪人。获取生物样本和组织核心服务受定期更新的生物样本收集和使用政策以及明确的退款机制的约束。 总的来说，所有四个TC部分都被所有5个研究项目大量使用，并为90多个模拟器启动的协议提供支持。核心请求CCSG提供400，966美元的支助，占其业务预算的24%。超过75%的用户是Moffitt成员和同行评审。

PROJECT SUMMARY (See instructions): The purpose of the Tissue Core (TC) is to help the facilitate the scientific needs of peer-reviewed. Cancer Center investigators by providing a collection of centralized services and technologies focused around high quality, richly-annotated human biospecimens. The TC is organized in four distinct operational sections that provide end-to-end biorepository services required for the collection, processing, annotation, storage and ultimate release of biospecimens. The TC-lntake & Acquisition section is responsible for the collection and initial annotation and de-identification of biospecimens for investigator driven and general banking protocols. The TC-Sample Processing Lab provides a wide variety of biospecimen processing services ranging from processing of blood to extraction of nucleic acids. The TC-Research Histology Services section supports CCSG investigators by offering basic histology services, research focused immunohistochemistry and construction of Tissue Microarray. The TC-Lifetime Cancer Screening operates as a Tissue Core satellite facility at the Center's Lifetime Cancer Screening and Prevention Center. The technical staff is responsible for operating and maintaining core instrumentation and technology. Over the last grant period, the Core established sophisticated, state-of-the-science technologies to support Immunohistochemistry (Ventana Discovery Automated System), automated, high-throughput nucleic acid extraction systems, (Qiagen) and most recently a Shared Instrumentation Grant funded automated freezer system. A new biospecimen inventory management system (LabVantage Biobanking) was also launched. The new LabVantage Biobanking system provides centralized comprehensive sample management, consent verification and biospecimen de-identification allowing the core to effectively serve as an institutional Honest Broker. Access to biospecimens and Tissue Core services are governed by regularly updated biospecimen collection and utilization policies and well-defined chargeback mechanism. Collectively, all four TC sections are heavily utilized by all 5 research programs and provide support for over 90 investigator-initiated protocols. The Core requests CCSG Support of $400,966, which is 24% of its operational budget. Over 75% of total users are Moffitt members and peer-reviewed.