Linking the physical and chemical characteristics of Qdots to their toxicity

将 Qdot 的物理和化学特性与其毒性联系起来

• 批准号: 8258515
• 负责人: Terrance J Kavanagh
• 金额: $ 3.01万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-24 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8258515
• 关键词: A/J Mouse; Air; Antibodies; Apoptosis; Arsenic; Benign; Bioinformatics; Biological; Biological Markers; Biology; Breathing; Budgets; Cadmium; Carbon; Carbon Nanotubes; Cell Culture System; Cell Line; Cell Survival; Cell model; Cells; Characteristics; Charge; Chemicals; Chemistry; Chromosome Mapping; Complementary DNA; Computer software; Conceptus; Custom; Data; Development; Dose; Drug Delivery Systems; Educational workshop; Elements; Embryo; Endothelial Cells; Engineering; Environment; Environmental Exposure; Epigenetic Process; Epithelial; Epithelial Cells; Epithelium; Excretory function; Exposure to; Female; Fluorescent Dyes; Gene Expression; Genetic Polymorphism; Genomics; Goals; Gold; Health; Heart; Heavy Metals; Hepatocyte; Histopathology; Human; Hypersensitivity; In Vitro; Inbred Mouse; Inbred Strains Mice; Inflammatory; Instruction; Journals; Kidney; Lead; Ligands; Link; Liquid substance; Liver; Lung; Maps; Materials Testing; Measures; Mercury; Metabolism; Methods; Mitochondria; Modeling; Modification; Mouse Strains; Mus; Nanosphere; Nanotubes; National Institute of Environmental Health Sciences; Nature; Necrosis; Occupational; Oxidation-Reduction; Oxidative Stress; Pathway Analysis; Pathway interactions; Phase; Poison; Protein Engineering; Proteins; Quantum Dots; Reporting; Request for Applications; Research; Rest; Risk; Risk Assessment; Schedule; Selenium; Semiconductors; Services; Shapes; Signal Pathway; Signal Transduction; Silver; Site; Small Interfering RNA; Spleen; Splenocyte; Sports Equipment; Staining method; Stains; Stress; Structure; Sunscreening Agents; Surface; Surface Properties; System; Techniques; Technology; Tellurium; Testing; Testis Brain; Thymus Gland; Tissues; Toxic effect; Toxicology; Transfection; Travel; Tubular formation; Vascular Endothelial Cell; Wound Healing; Writing; absorption; aerosolized; aqueous; base; biomineralization; cell type; ceric oxide; chemical property; cytokine; cytotoxicity; design; epigenomics; genetic technology; human tissue; imaging modality; in vivo; in vivo Model; insight; interest; iron oxide; kidney epithelial cell; lymph nodes; macrophage; male; meetings; member; metal oxide; molecular imaging; nanoGold; nanocrystal; nanofiber; nanomaterials; nanoparticle; nanoparticulate; nanorod; nanotoxicology; optical imaging; particle; physical property; predictive modeling; pregnant; programs; resistant strain; response; sertoli cell; titanium dioxide; uptake; web page

Engineered nanomaterials are being used or are being proposed for use in a large number of commercial products. Because of their highly diverse physical and chemical characteristics, and their small size, concerns have been raised regarding their potential to cause harm to human health or the environment. One important class of ENMs are quantum dots (Qdots) which are luminexcent semiconductor nanocrystals composed of heavy metal cores (e.g. CdSe, CdTe, HgTe) with cap and coating structures that vary greatly, depending upon the characteristics required for various applications. Certain characteristics are known to be determinants for the interaction of nanoparticles with cells and tissues, including shape, size, hydrophobieity, and surface charge. Because of the highly variable nature of these and other engineered nanomaterials, it will be nonetheless difficult to predict their toxicity. Modern advances in genomics, epigenetics and bioinformatics, and the availability of multiple strains of inbred mice with well characterized polymorphisms and regulatory sequences have made it possible to map and predict toxicity pathways for many toxic substances including nanomaterials. The research proposed herein will utilize in vitro and in vivo models of airway exposure to aerosolized quantum dot nanoparticles to 1) define the physical and chemical characteristics that govern their absorption, distribution, metabolism, excretion and toxicity; 2) using multiple inbred strains of mice, map toxicity pathways associated with these characteristics; and 3) incorporate the information that is obtained from these in vitro and in vivo models into a risk assessment paradigm that will be used to predict nanomaterial toxicity to humans. Such information will not only define which physical and chemical characteristics are important for the adverse biological effects of quantum dots, but will also simultaneously advance the fields of nanomaterial toxicology and functional epigenomics. These advances can then be used in safe design and manufacturing of nanomaterials so as to maximize their utility for many applications.

工程纳米材料正在被用于或正在被提议用于大量的商业用途