Generation and Characterization of Human Cytokine-Producing B Cell Effectors

产生人类细胞因子的 B 细胞效应子的生成和表征

基本信息

  • 批准号:
    8432311
  • 负责人:
  • 金额:
    $ 2.93万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-05-15 至 2013-04-30
  • 项目状态:
    已结题

项目摘要

Project Summary B cells are important for the generation of protective immunity and function at multiple discrete stages. The unique role that B cells play as antibody-producing cells is appreciated, however the other functions of B cells as antigen presenting cells, lymphoid tissue organizers and cytokine-producing effector or regulatory cells are much less understood and rarely studied. Recent work from our lab showed that cytokine producing effector B cells play crucial roles in the development of protective humoral and cellular immunity to infectious agents. We therefore hypothesized that there are specialized populations of B cells that have the ability to secrete cytokines and regulate immune responses. To address this hypothesis, we developed an in vitro culture system using primary mouse B cell to generate different classes of cytokine producing effector B cells. We characterized these in vitro generated cells and found markers that we subsequently used to identify the cytokine producing B cell subpopulations in vivo. For example, using markers identified in our in vitro cultures, we can now identify IFNγ producing B cell effectors in vivo and showed that these B cells are specifically induced in response to influenza infection and are elevated in number in some mouse models of autoimmunity. Thus, this approach has allowed us for the first time to visualize B cell effectors in vivo and has opened the door to functional and mechanistic studies that will reveal the roles for these cells in infectious and autoimmune disease. Despite the significant progress that we have made in our studies of mouse effector B cells, little is known about their human B cell counterparts. In fact, only very rudimentary studies have been conducted to date with human B cells. From these limited studies it is clear that human B cells have the potential to make cytokines under some stimulation conditions. However, these studies have been conducted with bulk peripheral blood B cells, thus the effector human B cells subsets remain to be identified. The goals of this proposal are to (i) to develop an in vitro culture system to phenotypically and functionally characterize human effector B cells and (ii) To use the information that we have obtained from the in vitro cultures to identify effector B cells in samples from healthy and autoimmune individuals.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Frances E. Lund其他文献

Transcription factor T-bet regulates the maintenance and differentiation potential of lymph node and lung effector memory B cell subsets
转录因子 T 细胞结合抑制因子调节淋巴结和肺效应记忆 B 细胞亚群的维持和分化潜能
  • DOI:
    10.1016/j.immuni.2025.05.021
  • 发表时间:
    2025-07-08
  • 期刊:
  • 影响因子:
    26.300
  • 作者:
    Christopher A. Risley;Michael D. Schultz;S. Rameeza Allie;Shanrun Liu;Jessica N. Peel;Anoma Nellore;Christopher F. Fucile;Christopher D. Scharer;Jeremy M. Boss;Troy D. Randall;Alexander F. Rosenberg;Frances E. Lund
  • 通讯作者:
    Frances E. Lund
IgM Memory Cells: First Responders in Malaria
  • DOI:
    10.1016/j.immuni.2016.08.005
  • 发表时间:
    2016-08-16
  • 期刊:
  • 影响因子:
  • 作者:
    Sara L. Stone;Frances E. Lund
  • 通讯作者:
    Frances E. Lund
This information is current as Infection in Mice Pneumocystis Clearance of T Cells for + Early Priming of CD 4 B Lymphocytes Are Required during the Feola
此信息是最新的,因为小鼠肺孢子虫感染在 Feola 期间需要清除 T 细胞以早期启动 CD 4 B 淋巴细胞
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M. Opata;M. Hollifield;Frances E. Lund;Troy D. Randall;Robert Dunn;B. Garvy;D. Feola
  • 通讯作者:
    D. Feola
Signaling through CD38 augments B cell antigen receptor (BCR) responses and is dependent on BCR expression.
通过 CD38 的信号传导可增强 B 细胞抗原受体 (BCR) 反应,并且依赖于 BCR 表达。
  • DOI:
    10.4049/jimmunol.157.4.1455
  • 发表时间:
    1996
  • 期刊:
  • 影响因子:
    4.4
  • 作者:
    Frances E. Lund;N.;K. M. Kim;M. Reth;Maureen Howard
  • 通讯作者:
    Maureen Howard
This information is current as MechanismsStrains of Influenza via Multiple B Cells Promote Resistance to Heterosubtypic and
该信息是最新的,因为机制流感菌株通过多个 B 细胞促进对异亚型和
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J. Rangel;D. Carragher;Ravi S. Misra;Kim L. Kusser;Louise Hartson;A. Moquin;Frances E. Lund;T. Randall
  • 通讯作者:
    T. Randall

Frances E. Lund的其他文献

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{{ truncateString('Frances E. Lund', 18)}}的其他基金

TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10642784
  • 财政年份:
    2020
  • 资助金额:
    $ 2.93万
  • 项目类别:
TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10431929
  • 财政年份:
    2020
  • 资助金额:
    $ 2.93万
  • 项目类别:
Tissue and organ specific human B cell immunity
组织和器官特异性人类 B 细胞免疫
  • 批准号:
    10265689
  • 财政年份:
    2020
  • 资助金额:
    $ 2.93万
  • 项目类别:
TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10032785
  • 财政年份:
    2020
  • 资助金额:
    $ 2.93万
  • 项目类别:
Identification and characterization of effector memory B cell populations that dominate memory responses to subsequent influenza infection and vaccination
效应记忆 B 细胞群的鉴定和表征,该细胞群主导对随后流感感染和疫苗接种的记忆反应
  • 批准号:
    10227903
  • 财政年份:
    2020
  • 资助金额:
    $ 2.93万
  • 项目类别:
TLR7 and TLR9-directed plasma cell formation: Dissecting the molecular basis for their differential dependence on IFN-induced signals
TLR7 和 TLR9 定向浆细胞形成:剖析它们对 IFN 诱导信号的差异依赖性的分子基础
  • 批准号:
    10214491
  • 财政年份:
    2020
  • 资助金额:
    $ 2.93万
  • 项目类别:
Identification and characterization of effector memory B cell populations that dominate memory responses to subsequent influenza infection and vaccination
效应记忆 B 细胞群的鉴定和表征,该细胞群主导对随后流感感染和疫苗接种的记忆反应
  • 批准号:
    10455632
  • 财政年份:
    2020
  • 资助金额:
    $ 2.93万
  • 项目类别:
Administrative Core
行政核心
  • 批准号:
    10395996
  • 财政年份:
    2019
  • 资助金额:
    $ 2.93万
  • 项目类别:
Characterization of virus-specific human B cell subsets in lymphoid and non-lymphoid tissues
淋巴和非淋巴组织中病毒特异性人类 B 细胞亚群的表征
  • 批准号:
    10592418
  • 财政年份:
    2019
  • 资助金额:
    $ 2.93万
  • 项目类别:
Tissue and organ specific human B cell immunity
组织和器官特异性人类 B 细胞免疫
  • 批准号:
    10592408
  • 财政年份:
    2019
  • 资助金额:
    $ 2.93万
  • 项目类别:

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人多能干细胞垂体前叶干细胞的产生和表征
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