Core C - FACS and Epigenetics Technology Core

以及在该中心开发的斑马鱼模型系统。这些技术，荧光激活的细胞分选 (FACS)和对少量细胞的表观遗传学分析，将提供一个前所未有的视角 造血术。染色质免疫沉淀和NEXT等技术的最新进展 世代测序(ChlP-seq)有望极大地提高我们对基因表达的理解 造血细胞。核心C将为中心成员提供对最新、最先进的单元的随时访问 分拣能力，并将进一步开发、应用和广泛使用最具创新性的方法 关于限制原代细胞数量的表观遗传学分析。核心C的努力将显著降低 阻碍了这些技术的应用。意义：详细了解正常造血过程中的血细胞发育和 各种疾病状态与人类疾病有直接关系，并将有助于开发新的 治疗方法。

and zebrafish model systems developed in the Center. These technologies, fluorescence activated ceil sorting (FACS) and epigenetic analysis on small numbers of cells, will provide an unprecedented view of hematopoiesis. The recent development of technologies such as chromatin immunoprecipitation and next generation sequencing (ChlP-seq) promises to dramatically enhance our understanding of gene expression in hematopoietic cells. CORE C will provide Center members with ready access to the latest, most advanced cell sorting capabilities, and will further develop, apply, and make widely available the most innovative approaches to epigenetic analyses on limiting numbers of primary cells. The efforts of CORE C will significantly lower the barrier to application of these technologies. Significance: A detailed understanding of blood cell development during normal hematopoiesis and various disease states has direct relevance to human disease and will aid in the development of new therapeutic approaches.