CORE--FUNCTIONAL GENOMICS

核心——功能基因组学

• 批准号: 7687431
• 负责人: YI ZHOU
• 金额: $ 12.18万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7687431
• 关键词: Anemia; Animal Disease Models; Bioinformatics; Biological Assay; Candidate Disease Gene; Complementary DNA; Dideoxy Chain Termination DNA Sequencing; Gene Expression; Gene Expression Regulation; Genes; Genome; Hematological Disease; Hematopoiesis; Human; In Situ Hybridization; Leadership; Length; Link; Maps; Microarray Analysis; Modeling; Patients; Pattern; Positioning Attribute; Research Personnel; Role; Sampling; Services; System; United States National Institutes of Health; Zebrafish; functional genomics; genome sequencing; improved; leukemia; mutant; positional cloning; tool; zebrafish genome

Specific Aims: The zebrafish genome is being sequenced by the Sanger Center, and Drs. Zon and Zhou are participating in the consortium to achieve a high quality assembly of the zebrafish genome sequence by 2006. We propose to comparatively evaluate gene expression patterns, profiles, and function during hematopoiesis in several vertebrate systems, improving our understanding of blood diseases such as anemias and leukemias. 1. Establish and compare gene expression patterns in vertebrate models by using in situ hybridization and microarray assays. 2. Apply bioinformatics tools to find gene regulation networks in animal disease models and human patient samples. 3. Map mutant genes and develop a reverse genetic strategy for zebrafish.

具体目的： 斑马鱼基因组由Sanger Center测序，Drs。 Zon和Zhou是 参与财团，到2006年获得斑马鱼基因组序列的高质量组装。我们建议在几个脊椎动物系统中相对评估造血过程中相对评估基因表达模式，谱和功能，从而提高我们对诸如贫血和白血病等血液疾病的理解。 1。通过使用原位杂交和微阵列分析，在脊椎动物模型中建立和比较基因表达模式。 2。应用生物信息学工具在动物疾病模型和人类中找到基因调节网络 病人样本。 3。映射突变基因并为斑马鱼开发反向遗传策略。