CORE--FUNCTIONAL GENOMICS

具体目标： 斑马鱼基因组正在由桑格中心进行测序，Zon和Zhou博士正在 参与该联盟，以在2006年之前实现斑马鱼基因组序列的高质量组装。我们建议比较评估基因的表达模式，配置文件和造血功能在几个脊椎动物系统，提高我们的血液疾病，如贫血和白血病的理解。1.利用原位杂交和微阵列分析建立和比较脊椎动物模型中的基因表达模式。2.应用生物信息学工具寻找动物疾病模型和人类的基因调控网络 患者样本。3.斑马鱼突变基因图谱及反向遗传策略的研究。

Specific Aims: The zebrafish genome is being sequenced by the Sanger Center, and Drs. Zon and Zhou are participating in the consortium to achieve a high quality assembly of the zebrafish genome sequence by 2006. We propose to comparatively evaluate gene expression patterns, profiles, and function during hematopoiesis in several vertebrate systems, improving our understanding of blood diseases such as anemias and leukemias. 1. Establish and compare gene expression patterns in vertebrate models by using in situ hybridization and microarray assays. 2. Apply bioinformatics tools to find gene regulation networks in animal disease models and human patient samples. 3. Map mutant genes and develop a reverse genetic strategy for zebrafish.