CORE--FUNCTIONAL GENOMICS

核心——功能基因组学

• 批准号: 7494130
• 负责人: YI ZHOU
• 金额: $ 12.63万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7494130
• 关键词: Anemia; Animal Disease Models; Bioinformatics; Biological Assay; Candidate Disease Gene; Complementary DNA; Dideoxy Chain Termination DNA Sequencing; Gene Expression; Gene Expression Regulation; Genes; Genome; Hematological Disease; Hematopoiesis; Human; In Situ Hybridization; Leadership; Length; Link; Maps; Microarray Analysis; Modeling; Patients; Pattern; Positioning Attribute; Research Personnel; Role; Sampling; Services; System; United States National Institutes of Health; Zebrafish; functional genomics; genome sequencing; improved; leukemia; mutant; positional cloning; tool; zebrafish genome

Specific Aims: The zebrafish genome is being sequenced by the Sanger Center, and Drs. Zon and Zhou are participating in the consortium to achieve a high quality assembly of the zebrafish genome sequence by 2006. We propose to comparatively evaluate gene expression patterns, profiles, and function during hematopoiesis in several vertebrate systems, improving our understanding of blood diseases such as anemias and leukemias. 1. Establish and compare gene expression patterns in vertebrate models by using in situ hybridization and microarray assays. 2. Apply bioinformatics tools to find gene regulation networks in animal disease models and human patient samples. 3. Map mutant genes and develop a reverse genetic strategy for zebrafish.

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