CORE--FUNCTIONAL GENOMICS

具体目标： 桑格中心正在对斑马鱼的基因组进行测序，Zon博士和周博士正在 参与该联盟，在2006年前实现斑马鱼基因组序列的高质量组装。我们建议比较评估几种脊椎动物系统在造血过程中的基因表达模式、图谱和功能，以提高我们对贫血和白血病等血液疾病的理解。1.利用原位杂交和基因芯片技术建立和比较脊椎动物模型的基因表达谱。2.应用生物信息学工具在动物疾病模型和人类中寻找基因调控网络 病人样本。3.定位斑马鱼的突变基因并开发反向遗传策略。

Specific Aims: The zebrafish genome is being sequenced by the Sanger Center, and Drs. Zon and Zhou are participating in the consortium to achieve a high quality assembly of the zebrafish genome sequence by 2006. We propose to comparatively evaluate gene expression patterns, profiles, and function during hematopoiesis in several vertebrate systems, improving our understanding of blood diseases such as anemias and leukemias. 1. Establish and compare gene expression patterns in vertebrate models by using in situ hybridization and microarray assays. 2. Apply bioinformatics tools to find gene regulation networks in animal disease models and human patient samples. 3. Map mutant genes and develop a reverse genetic strategy for zebrafish.