Integrated Optical Coherence Tomography and Microscopy for Molecular-Targeted Ima

用于分子靶向成像的集成光学相干断层扫描和显微镜

• 批准号: 8472624
• 负责人: Chao Zhou
• 金额: $ 24.9万
• 依托单位: LEHIGH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8472624
• 关键词: Animal Model; Antibodies; Binding; Biodistribution; Biological; Cancer Detection; Cardiovascular Diseases; Cell Culture Techniques; Cells; Confocal Microscopy; Contrast Media; Data Set; Detection; Development; Epidermal Growth Factor Receptor; Frequencies; Glutamate Carboxypeptidase II; Goals; Histology; Histopathology; Human; Image; Imagery; Imaging Techniques; Imaging technology; Instruction; Lasers; Ligands; Methods; Microscopy; Molecular; Molecular Target; Morphology; Nanotechnology; Neurodegenerative Disorders; Noise; Optical Coherence Tomography; Optics; Pathology; Performance; Phase; Proteins; Protocols documentation; Research; Resolution; Screening for cancer; Sensitivity and Specificity; Signal Transduction; Source; Specimen; Specimen Handling; Speed; Staging; Structure; System; Techniques; Technology; Therapeutic; Time; Tissues; Toxic effect; absorption; base; cancer cell; cytotoxicity; design; imaging modality; improved; in vivo; nanoparticle; neoplastic; novel; phase change; programs; protein expression; small molecule; tool; tumor; tumor progression

Optical coherence tomography (OCT) is a powerful tool for assessing three-dimensional (3D) tissue architectural morphology in vivo and in real-time, with a resolution approaching that of standard histopathology. Optical coherence microscopy (OCM) combines OCT with confocal microscopy in order to achieve high transverse resolution and 3D visualization of cellular features. However, current OCT and OCM imaging technologies have not been able to leverage the advances in molecular-targeted contrast agents because there is no. known method to generate molecular contrast using OCT and OCM. Our hypothesis is that 3D, multi-scale OCT and OCM, in combination with molecular-targeted contrast agents, will improve the sensitivity and specificity of early cancer detection. The goal of this program is to develop the technology that will enable molecular contrast for 3D-0CT and OCM imaging, building upon preliminary studies. This program will vertically integrate technical development in OCT and OCM, and leverage recent advances in molecular-targeted contrast agents and nanotechnology. The successful completion of this program will: 1) transform the OCT field by enabling molecularly sensitive contrast and 3D structural imaging; 2) open the way for the highly sensitive and specific detection of cancer markers that can be readily combined with photothermal therapeutic techniques; and 3) serve as a launching point for additional OCT and OCM studies of other pathologies associated with abnormal protein expression levels, such as neurodegenerative and cardiovascular diseases. These advances will enable both the structure and pathological states of tissue to be imaged in 3D, in vivo, in real time, and with micron-level spatial resolutions at multiple scales.

光学相干断层扫描（OCT）是评估体内和实时的三维（3D）组织架构形态的强大工具，其分辨率接近标准组织病理学的分辨率。光学相干显微镜（OCM）将OCT与共聚焦显微镜结合在一起，以实现高横向分辨率和细胞特征的3D可视化。但是，由于没有。使用OCT和OCM生成分子对比的已知方法。我们的假设是3D，多尺度OCT和OCM结合了分子靶向对比剂，将提高早期癌症检测的敏感性和特异性。该计划的目的是开发将在初步研究的基础上为3D-0CT和OCM成像的分子对比的技术。 该计划将垂直整合OCT和OCM的技术发展，并利用分子针对的对比剂和纳米技术的最新进展。该程序的成功完成将：1）通过启用分子敏感对比和3D结构成像来改变OCT场； 2）为高度敏感和特异性检测癌症标志物开辟道路，这些癌症标志物可以与光热治疗技术结合使用；和3）作为与异常蛋白表达水平（例如神经退行性和心血管疾病）相关的其他病理学的其他OCT和OCM研究的发射点。这些进步将使组织的结构和病理状态能够在3D，体内，实时成像，并在多个尺度上实时使用微米级的空间分辨率。