Acquisition of a Transmission Electron Microscope

购买透射电子显微镜

• 批准号: 8246562
• 负责人: MARK WINEY
• 金额: $ 59万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246562
• 关键词: 3-Dimensional; Aging; Binding Proteins; Biological; Cellular biology; Collaborations; Collection; Colorado; Computers; Developmental Biology; Diagnostic; Eagles; Electron Microscope; Electron Microscopy; Endosomes; Freeze Fracturing; Funding; Heart Diseases; Image; Institution; Malignant Neoplasms; Membrane Proteins; Microscope; Microscopy; Microtubules; Minor; Molecular; Molecular Biology; Molecular Structure; Mutation; Myosin ATPase; Neurodegenerative Disorders; Plastics; Preparation; Resolution; Services; Specimen; Staging; Staining method; Stains; Structure; System; Therapeutic; Twin Multiple Birth; United States National Institutes of Health; Universities; Virus; digital; electron tomography; instrument; lens; nanoparticle; protein structure; technology development; transmission process; virology

DESCRIPTION (provided by applicant): The MCDB Electron Microscopy Service, located in the Department of Molecular, Cellular and Developmental Biology at the University of Colorado in Boulder, seeks funding to purchase a FEI Tecnai G2 Spirit Bio Twin Transmission Electron Microscope (TEM) from the FEI Company. A new TEM is needed to replace our 23-year-old Philips CM10 TEM, an underperforming microscope that is increasingly difficult to maintain and is currently serving the users of the MCDB EM Service. The FEI G2 Spirit Bio Twin electron microscope is a very versatile 120 KV TEM that is appropriate for most electron microscopy (EM) applications in cellular and molecular microscopy. Its high-contrast lens is well suited to a wide range of specimens and is ideal for low-contrast biological specimens such as negatively stained cellular preparations, high- resolution freeze-fracture replicas and conventional thin plastic sections. The Tecnai G2 Spirit BioTwin is equipped with a computer controlled +/- 70 degree tiltable goniometer stage that can accommodate conventional specimen holders, rotating holders for control of the tilt axis, high- tilt-dual-axis holders for electron tomography(3-dimensional imaging) and state-of-the-art LN2 cryo-holders for imaging vitrified specimens. Seven NIH-funded major users are submitting this application: Dr. Bruce Eaton and Dr. Daniel Feldheim (collaboration on bio-metalic nanoparticles), Dr. Robert Garcea (virus structure and assembly), Dr. Andreas Hoenger (microtubule binding protein structure, technology development), Dr. Leslie Leinwand (myosin mutations and heart disease), Dr. Greg Odorizzi (endosome assembly and structure), Dr. Michael Stowell (membrane protein structure), and Dr. Mark Winey (structure and assembly of MTOCs). The microscope will also service a collection of minor users, both from the University of Colorado and from other institutions. All of the users require the 5-Angstrom point-to-point resolution obtainable with this microscope and the requested Eagle 2K x 2K digital camera system. Many of their projects depend upon the convenient control of the specimen tilt axis provided by this instrument's stage and the requested specimen holders. Altogether, the major and minor users study a wide variety of cell biology topics or molecular structures that are relevant to virology, cancer, heart disease, aging, neurodegenerative diseases, and diagnostic and therapeutic strategies.

描述（由申请人提供）：MCDB电子显微镜服务，位于博尔德的科罗拉多大学分子、细胞和发育生物学系，寻求资金从FEI公司购买FEI Tecnai G2 Spirit Bio Twin透射电子显微镜（TEM）。我们需要一台新的TEM来取代我们使用了23年的Philips CM 10 TEM，这是一台性能不佳的显微镜，越来越难以维护，目前正在为MCDB EM服务的用户提供服务。FEI G2 Spirit Bio Twin电子显微镜是一款非常通用的120 KV TEM，适用于细胞和分子显微镜中的大多数电子显微镜（EM）应用。它的高对比度透镜非常适合于各种标本，是低对比度生物标本的理想选择，例如负染色细胞制备物、高分辨率冷冻断裂复制品和传统薄塑料切片。Tecnai G2 Spirit BioTwin配备了计算机控制的+/- 70度可倾斜测角器载物台，可容纳传统的标本夹持器、用于控制倾斜轴的旋转夹持器、用于电子断层扫描（三维成像）的高倾斜双轴夹持器和用于玻璃化标本成像的最先进的LN 2冷冻夹持器。七个NIH资助的主要用户正在提交此应用程序：布鲁斯伊顿博士和丹尼尔费尔德海姆博士（生物金属纳米颗粒合作），罗伯特·加尔恰博士（病毒结构和组装），Andreas Hoenger博士（微管结合蛋白结构，技术开发），Leslie Leinwand博士（肌球蛋白突变和心脏病），Greg Odorizzi博士（核内体组装和结构）、Michael Stowell博士（膜蛋白结构）和Mark Winey博士（MTOC的结构和组装）。该显微镜还将为来自科罗拉多大学和其他机构的一些小用户提供服务。所有的用户都需要5埃的点对点分辨率与此显微镜和所要求的鹰2K x 2K数码相机系统获得。他们的许多项目依赖于该仪器的载物台和所需的样品架提供的样品倾斜轴的方便控制。总的来说，主要和次要用户研究各种各样的细胞生物学主题或与病毒学，癌症，心脏病，衰老， 神经退行性疾病以及诊断和治疗策略。