TripleTOF 5600 Hybrid Mass Spectrometer

描述（由申请人提供）：该提案要求使用AB SCIEX TripleTOF 5600质谱仪-新一代四极杆飞行时间混合串联质谱仪系统，该系统将用于推进许多NIH资助的对人类健康重要的项目，例如：鉴定慢性盆腔疼痛、肾盂输尿管连接部（UPJ）梗阻和胰腺炎的生物标志物，了解婴儿猝死综合征（SIDS）和脊髓性肌萎缩症（SMA）的病理生理学，识别与炎性疼痛相关的感觉神经元上的细胞因子和受体，并促进对最小细胞周期和多位点磷酸化的系统生物化学的理解。所要求的质谱仪是一种市售仪器，具有独特的功能组合，使其成为各种所述项目的理想选择;这些功能包括非常高的扫描速度，出色的灵敏度以及MS 1和MS 2域中出色的分辨率和质量精度。这种规格组合使TripleTOF 5600质谱仪成为基于同量异位素质量标记（如iTRAQ和/或TMT）的多路定量蛋白质组学实验的首选仪器。由于所描述的绝大多数项目都采用这种定量策略，因此获得这种最先进的混合串联质谱仪将显著推进所提出的研究项目。拟议的仪器将为波士顿儿童医院的蛋白质组学中心增加急需和期待已久的额外和最先进的能力，该中心拥有老化的蛋白质组学级仪器。收购AB SCIEX TripleTOF 5600系统将从两个方面使蛋白质组学中心受益：1）减少目前两周的样品等待时间; 2）极大地改善使用CHB蛋白质组学中心的研究人员可用的技术。目前用于这些定量实验的四极TOF仪器比目前最先进的仪器老两代，因此由于仪器和相关技术的老化，其能力显著降低。因此，所要求的仪器将对整个波士顿儿童医院的基础和转化研究界大有裨益。该仪器将被安置在波士顿儿童医院的蛋白质组学中心。蛋白质组学中心训练有素的专家人员拥有多年的AB SCIEX仪器经验，将监督和管理仪器的操作和维护，从而确保有效的仪器时间管理，最大限度地减少闲置或停机时间，从而推进拟议和未来的项目。 公共卫生相关性：AB SCIEX TripleTOF 5600四极杆飞行时间质谱仪将为波士顿儿童医院的蛋白质组学中心增加急需的最先进能力。它将被应用于许多NIH资助的对人类健康重要的转化和基础项目。其中包括：确定慢性盆腔疼痛，肾盂输尿管连接部（UPJ）梗阻和胰腺炎的生物标志物，了解婴儿猝死综合征（SIDS）和脊髓性肌萎缩症（SMA）的病理生理学，炎症性疼痛的研究和推进最小细胞周期的理解和多位点磷酸化的系统生物化学。

DESCRIPTION (provided by applicant): This proposal requests an AB SCIEX TripleTOF 5600 mass spectrometer - a new generation quadrupole time- of-flight hybrid tandem mass spectrometer system that will be utilized to advance numerous NIH-funded projects important to human health, for example: identifying biomarkers for chronic pelvic pain, Ureteropelvic junction (UPJ) obstruction and pancreatitis, understanding the pathophysiology of Sudden Infant Death Syndrome (SIDS) and Spinal Muscular Atrophy (SMA), the identification of cytokines and receptors on sensory neurons associated with inflammatory pain and advancing the comprehension of the minimal cell cycle and the systems biochemistry of multisite phosphorylation. The requested mass spectrometer is a commercially available instrument that has unique combination of features making it ideal for the various described projects; these features include very high scan speed, excellent sensitivity and outstanding resolution and mass accuracy in the MS1- and MS2-domain. This combination of specifications makes the TripleTOF 5600 mass spectrometer the instrument of choice for multiplexed quantitative proteomics experiments based on isobaric mass tagging such as iTRAQ and/or TMT. Since the vast majority of the described projects are applying this quantitative strategy, acquisition of this stae- of-the-art hybrid tandem mass spectrometer will significantly advance the proposed research projects. The proposed instrument will add critically needed and long awaited additional and state-of-the-art capacity to the Proteomics Center at Children's Hospital Boston, which has an ageing fleet of proteomics-grade instruments. Acquisition of the AB SCIEX TripleTOF 5600 System will benefit the Proteomic Center in two ways 1) alleviate the wait time for samples which is two weeks at the moment 2) vastly improve the technology available to researchers who use the CHB Proteomics Center. The current quadrupole TOF instrument used for these quantitative experiments is two generations older than the current state-of-the-art machine and thus has significantly diminished capabilities due to the age of the instrument and technology associated. As such, the requested instrument will be greatly beneficial for the basic and translational research community at Children's Hospital Boston at large. The instrument will be housed in the Proteomics Center at Children's Hospital Boston. The well-trained expert personnel of the Proteomics Center, who have many years of experience with AB SCIEX instruments, will oversee and administer the operation and maintenance of the instrument thereby ensuring efficient instrument time management with minimal idle or downtime thereby advancing the proposed and future projects. PUBLIC HEALTH RELEVANCE: The AB SCIEX TripleTOF 5600 quadrupole time-of-flight mass spectrometer will add critically needed state-of the-art capacity to the Proteomics Center at Children's Hospital Boston. It will be applied to numerous NIH-funded translational and basic projects important to human health. These include: identifying biomarkers for chronic pelvic pain, Ureteropelvic junction (UPJ) obstruction and pancreatitis, understanding the pathophysiology of Sudden Infant Death Syndrome (SIDS) and Spinal Muscular Atrophy (SMA), studies of inflammatory pain and advancing the comprehension of the minimal cell cycle and the systems biochemistry of multisite phosphorylation.