Watching a vertebrate brain learn and behave in a virtual environment
观察脊椎动物大脑在虚拟环境中学习和行为
基本信息
- 批准号:8471387
- 负责人:
- 金额:$ 84.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-09-30 至 2016-07-31
- 项目状态:已结题
- 来源:
- 关键词:AlgorithmsAnestheticsAnimalsBehaviorBehavioralBiological AssayBrainCellsComputersCuesDataElectrodesEnvironmentFishesLaser Scanning MicroscopyLearningMemoryMonitorMotor NeuronsNeuronsParalysedPhotonsPopulationPreparationResolutionStimulusSuctionSwimmingSystemTrainingTransgenic OrganismsVisualZebrafishabstractingawakebasecalcium indicatorrelating to nervous systemsensory feedbackvirtualwater flow
项目摘要
DESCRIPTION
Abstract:
In order to study the neural basis of navigation, learning, and memory, it is important to simultaneously record the activity of large populations of neurons involved in execution of behavior. This has been difficult or impossible in awake, freely moving preparations and the anesthetics and paralytics used to facilitate recordings often alter or abolish the relevant behaviors. I propose to establish a fictive swimming assay that allows paralyzed larval zebrafish to navigate through- and interact with a virtual environment. The sensory feedback provided to the animals will consist of visual cues through video projection screens and water flow provided by a computer controlled valve system. The fish are allowed to control these stimuli via activity in their motor-neurons recorded by a set of external suction electrodes. This arrangement makes it possible for immobilized fish to navigate a virtual environment entirely through power of activity in their motorneurons. As a first step we will combine this assay with 2-photon laser-scanning microscopy and allow transgenic fish lines that express genetically encoded calcium indicators in all neurons to navigate through virtual environments while neuronal activity is monitored. The small size and perfect translucence of the larval zebrafish provides in principle no barrier or restriction to these recordings and thus we have access to the whole brain at single cell resolution during these behavioral tasks. We can thus independently monitor the activities of hundreds or thousands of neurons as animals navigate their virtual worlds. As a subsequent step we will implement different learning assays that the animals have to execute in this virtual environment. In principle this will allow us to follow the flow of neural information in a single animal, before, during and after specific training sessions. The large volumes of resulting data will be analyzed and distilled with automated algorithms and the results used to sketc
描述
摘要:
为了研究导航、学习和记忆的神经基础,同时记录参与行为执行的大量神经元的活动是很重要的。这在清醒的、自由活动的准备中是困难或不可能的,用于促进记录的麻醉剂和麻痹剂经常改变或取消相关行为。我建议建立一个虚构的游泳试验,使瘫痪的斑马鱼幼虫导航通过-并与虚拟环境进行交互。提供给动物的感觉反馈将包括通过视频投影屏幕的视觉提示和由计算机控制的阀门系统提供的水流。这些鱼被允许通过一组外部吸力电极记录的运动神经元的活动来控制这些刺激。这种安排使得固定的鱼完全通过其运动神经元的活动能力来导航虚拟环境成为可能。作为第一步,我们将结合联合收割机这种测定与双光子激光扫描显微镜,并允许转基因鱼线,表达遗传编码的钙指标,在所有神经元导航通过虚拟环境,而神经元的活动进行监测。斑马鱼幼体的小尺寸和完美的透明度原则上对这些记录没有障碍或限制,因此我们在这些行为任务期间可以以单细胞分辨率访问整个大脑。因此,我们可以独立地监测动物在虚拟世界中导航时数百或数千个神经元的活动。作为后续步骤,我们将实施不同的学习试验,动物必须在这个虚拟环境中执行。原则上,这将使我们能够在特定的训练课程之前,期间和之后跟踪单个动物的神经信息流。将使用自动算法分析和提取大量的结果数据,并将结果用于绘制草图。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Florian Engert其他文献
Florian Engert的其他文献
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{{ truncateString('Florian Engert', 18)}}的其他基金
Genetic and neural mechanisms underlying emerging social behavior in zebrafish
斑马鱼新兴社会行为的遗传和神经机制
- 批准号:
10306905 - 财政年份:2021
- 资助金额:
$ 84.5万 - 项目类别:
Sensorimotor processing, decision making, and internal states: towards a realistic multiscale circuit model of the larval zebrafish brain
感觉运动处理、决策和内部状态:建立幼虫斑马鱼大脑的真实多尺度电路模型
- 批准号:
9444232 - 财政年份:2017
- 资助金额:
$ 84.5万 - 项目类别:
Sensorimotor processing, decision making, and internal states: towards a realistic multiscale circuit model of the larval zebrafish brain
感觉运动处理、决策和内部状态:建立幼虫斑马鱼大脑的真实多尺度电路模型
- 批准号:
10241477 - 财政年份:2017
- 资助金额:
$ 84.5万 - 项目类别:
The Heart and the Mind: An Integrative Approach to Brain-Body Interactions in the Zebrafish
心脏和思想:斑马鱼脑体相互作用的综合方法
- 批准号:
10525427 - 财政年份:2017
- 资助金额:
$ 84.5万 - 项目类别:
Sensorimotor processing, decision making, and internal states: towards a realistic multiscale circuit model of the larval zebrafish brain
感觉运动处理、决策和内部状态:建立幼虫斑马鱼大脑的真实多尺度电路模型
- 批准号:
9570757 - 财政年份:2017
- 资助金额:
$ 84.5万 - 项目类别:
The Heart and the Mind: An Integrative Approach to Brain-Body Interactions in the Zebrafish
心脏和思想:斑马鱼脑体相互作用的综合方法
- 批准号:
10686975 - 财政年份:2017
- 资助金额:
$ 84.5万 - 项目类别:
What is going on in the fish's brain? Characterization and Modeling of Neural Dynamics (CNS and ANS and ICNS)
鱼的大脑里发生了什么?
- 批准号:
10525434 - 财政年份:2017
- 资助金额:
$ 84.5万 - 项目类别:
What is going on in the fish's brain? Characterization and Modeling of Neural Dynamics (CNS and ANS and ICNS)
鱼的大脑里发生了什么?
- 批准号:
10686992 - 财政年份:2017
- 资助金额:
$ 84.5万 - 项目类别:
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