NIH-Owned Chimpanzee Research Resource at the SNPRC

描述（由申请人提供）：黑猩猩与人类在遗传、生理、生化和免疫学方面的相似性使它们成为针对人类健康许多方面的生物医学研究的独特模型，其中包括开发传染病（例如HBV、HCV、HIV）的预防和治疗方法，测试专门为人类使用而设计的药物的效果，以及测试人源化单克隆抗体的安全性和有效性。该项目的总体目标是维持由209只黑猩猩组成的ncrr拥有的研究资源，并对其进行管理，以满足国家生物医学研究的需要。我们将追求以下具体目标：1)维持一个稳定、健康、明确的黑猩猩种群，并使它们可用于研究；2)维持一群持续感染HBV、HCV或HIV的黑猩猩，用于肝炎和艾滋病的研究，并测试新的治疗方法的效果；3)从所有209只黑猩猩的肝脏样本，包括mrna和microrna，以及36只慢性丙型肝炎病毒感染或已清除感染的黑猩猩的PBMC样本中开发一种独特的完全测序转录组资源；(4)制定对生物医学研究具有重要意义的生理特征的纵向定量测量方法，并将其与Aim 3的序列数据和健康状况相联系进行分析；5)建立一个数据库，其中包含有关黑猩猩的信息和根据目标3和4获得的数据，并将其提供给国家生物医学研究界，用于研究目的和选择实验对象；6)建立一个项目，教育公众认识到黑猩猩在生物医学研究中的重要性。通过实现这些目标，我们将建立一个在所有黑猩猩研究资源中独一无二的黑猩猩资源，使研究人员首次能够在实验设计和基因组分析中利用黑猩猩个体的基因组序列数据。此外，该教育项目将有助于公众了解在生物医学研究中对黑猩猩的照顾和使用，以及对本应用程序中提出的资源的需求。

DESCRIPTION (provided by applicant): The genetic, physiological, biochemical, and immunological similarities of chimpanzees to humans make them unique models for biomedical research directed at many aspects of human health including, among others, developing preventions and treatments for infectious diseases (e.g., HBV, HCV, HIV), testing the effects of drugs specifically designed for use in humans, and testing the safety and efficacy of humanized monoclonal antibodies. The overall objective of this project is to maintain an NCRR-owned research resource consisting of 209 chimpanzees and to manage it to serve the national biomedical research needs. We will pursue the following specific aims: 1) To maintain a stable, healthy, well defined population of chimpanzees and to make them available for research; 2) To maintain a cohort of well characterized chimpanzees persistently infected with HBV, HCV or HIV for research on hepatitis and AIDS and for testing the efficacy of new therapeutic approaches; 3) To develop a unique resource of fully sequenced transcriptomes from liver samples of all 209 chimpanzees, including mRNAs and microRNAs, and from PBMC samples of a subset of 36 chimpanzees that are chronically infected with HCV or that have cleared the infection; 4) To develop longitudinal quantitative measures of physiological characteristics that are of importance to biomedical research and to analyze them in relation to sequence data from Aim 3 and in relation to health status; 5) To develop a data base that contains information about the chimpanzees and the data obtained under Aims 3 and 4, and to make it available to the national biomedical research community for research purposes and for selection of experimental subjects; 6) To establish a program for educating the public as to the importance of chimpanzees in biomedical research. Via fulfilling these aims, we will establish a chimpanzee resource that is unique among all chimpanzee research resources in enabling investigators for the first time to exploit genome sequence data from individual chimpanzees in experimental design and in genomic analyses. In addition, the educational program will contribute to the public's understanding of the care and use of chimpanzees in biomedical research, and the need for resources such as the one proposed in this application.