Web-based Atlas of Zebrafish Microanatomy as a Community Resource

描述（由申请人提供）：斑马鱼现在是一种常见的、非哺乳动物的人类疾病模型，因为它具有小尺寸、透明和强大的遗传工具等实验特征。为了响应美国国立卫生研究院赞助的斑马鱼和水生模型工作组的建议，美国科学基础设施的一个重要组成部分正在以基于网络的解剖学、组织学和病理学图谱的形式建立起来。该地图集可从www.zfatlas.psu.edu和俄勒冈大学的斑马鱼信息网络（ZFIN） （www.zfin.org）获取。正常解剖将作为研究由遗传缺陷和疾病引起的异常解剖的关键背景。图集的人员、成像、计算机硬件和网站构成了基础设施，供用户在正常的背景下以2D和3D方式观察突变/变形/患病的斑马鱼。具体目标1是从一个已建立的突变集合开始，将异常表型整合到图谱中，创建一个基于网络的、最先进的虚拟幻灯片的远程标记的非破坏性机制，允许以不同的功率查看组织学组织切片，将斑马鱼图谱与其他基于网络的资源（包括斑马鱼网络资源之家（www.zfin.org））整合，并使用网络界面显示一系列突变表型。具体目标2是开发分析和显示3D斑马鱼数据的机制。我们将从斑马鱼的数字3D表示中分割和标记不同的器官，并探索，测试和实现虚拟载玻片和由微ct成像在亚细胞分辨率下创建的斑马鱼3D表示之间的集成接口。具体目标3是为跨模型系统的结构和功能集成建立基础。该目标将利用斑马鱼、小鼠和人类作为感兴趣的生物，并使用特定的骨骼、眼睛和皮肤颜色突变作为描述模型系统在理解生物功能和疾病中的作用的范例。这个目标将集中在所有三个物种共享的正常组织，然后是所有三个物种共享的病理。这个社区资源的整合将作为所有模型系统网站的模型，服务于NIH的多个任务。

DESCRIPTION (provided by applicant): The zebrafish is now a common, non-mammalian model of human disease due to experimental features such as small size, transparency, and powerful genetic tools. In response to NIH-sponsored Zebrafish and Aquatic Models working groups' recommendations, an important piece of American scientific infrastructure is being established in the form of a web-based atlas of anatomy, histology, and pathology. This atlas will be accessible from www.zfatlas.psu.edu and the Zebrafish Information Network (ZFIN) at the University of Oregon (www.zfin.org). Normal anatomy will serve as critical context for the study of abnormal anatomy caused by genetic deficiencies and disease. The atlas' personnel, imaging and computer hardware and web site comprise the infrastructure for users to view mutant/morphant/diseased zebrafish in the context of normal, in both 2D and 3D. Specific Aim 1 is to integrate abnormal phenotypes into the atlas starting with an established mutant collection, to create a nondestructive mechanism for remote labeling of web-based, state-of-the-art virtual slides that allows viewing of histological tissue sections at different powers, to integrate the zebrafish atlas with other web-based resources including the home of zebrafish web-based resources (www.zfin.org), and to use the web interface to display a range of mutant phenotypes. Specific Aim 2 is to develop mechanisms for analyzing and displaying 3D zebrafish data. We will segment and label different organs from digital 3D representations of zebrafish, and explore, test, and implement integrated interfaces between virtual slides and 3D representations of zebrafish created by microCT imaging at subcellular resolutions. Specific Aim 3 is to build a foundation for structural and functional integration across models systems. This aim will utilize zebrafish, mouse, and human as the organisms of interest, and use specific skeletal, eye, and skin color mutations as a paradigm for describing the roles of model systems in understanding a biological function and disease. This aim will focus on normal tissues shared by all three species followed by pathology shared by all three species. The integrations of this community resource will serve as a model for all model system web sites, serving multiple missions across the NIH. PUBLIC HEALTH RELEVANCE (provided by applicant): Compelling experimental features have made the zebrafish a model for a wide range of human maladies, including cancer, heart disease, and infection. The atlas (www.zfatlas.psu.edu) contains high-resolution 2D and 3D representations of normal and, as proposed, abnormal zebrafish. By cross-referencing with other model systems, the atlas will also provide infrastructure for integrative, multidisciplinary projects.