National Resource for Aplysia

该提案说明了继续实施《亚洲国家资源》，其总体目标是： 提供始终如一的高品质养殖海兔Aaprisia californica（及其养殖红藻食品）， 研究人员A.加州是一个重要的非脊椎动物（后鳃类软体动物）的健康模式系统， 相关的研究，主要是在行为和学习的神经生理学。我们将继续生产 动物进行研究，并进行基础研究，旨在探索新的模型用途，并提高 resource.有四个次级项目/具体目标： 1.生产-向美国和国际研究人员出售动物预计将继续在 目前每年有25，000到30，000只动物。该资源提供高质量的 A.加利福尼亚州的所有生命阶段，从鸡蛋到成熟的成年人饲养动物在精心控制的 实验室条件所有生命阶段都有足够的数量，以满足用户的需求， 一年，消除了对野生动物的依赖，这些动物季节性有限， 年龄和健康状况不明。 2.生产研究计划-在过去的12年里，资源不断得到改善， 研究以优化A.加利福尼亚州。拟议的研究计划 将专注于鸡蛋质量的测量和改善。 3.动物健康监测-我们将继续开展基于动物健康筛查的监测计划 参数，以确保快速检测和完整记录任何疾病过程， 发生在孵化场动物的任何发育阶段。任何疾病综合征和疑似 将对观察到的病原体进行调查，并采取适当的控制措施。 4.发育神经生理学-我们将开始通过研究 调节尾部感觉神经元输入的D-Asp和其他激动剂激活离子电流的变化 退缩反射，使用单细胞电压钳位并记录完整反射。 通过这种生产与基础研究的结合，我们将完善模型体系， 对其他研究领域的帮助。 相关性（参见说明）： 加州海兔，是一种大型的海洋蜗牛，有一个非常简单的神经系统。的 每年，美国国家动物资源中心会饲养并运送25,000到30000只这样的动物到美国的实验室 在神经生物学和行为方面进行基础研究。这项建议将 继续生产这些动物，并扩大其在衰老研究方面的用途， 老年痴呆症

This proposal describes the continuation of the National Resource for Aplysia, whose overall goal is to provide consistently high-quality cultured sea hares Aplysia californica (and their cultured red algal food) to researchers. A. californica is an important non-vertebrate (Opisthobranch mollusk) model system for health- related research, primarily in the neurophysiology of behavior and learning. We will continue to produce animals for research and to conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-projects/specific aims: 1. Production - Sales of animals to researchers in the US and internationally is projected to continue at present levels of 25,000 to 30,000 animals per year. This resource makes available high-quality A. californica at all life stages from eggs through mature adults by rearing animals in carefully controlled laboratory conditions. All life-stages are available in sufficient quantities to meet user demand throughout the year, eliminating reliance on wild-collected animals which have limited seasonal availability and are of unknown age and health status. 2. Production research programs - The resource has been continuously improved over the past 12 years by research to optimize rearing conditions for most life stages of A. californica. The proposed research program will focus on measurement and improvement of egg quality. 3. Animal Health Monitoring - We will continue a monitoring program based on screening animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animals at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied. 4. Developmental Neurophysiology - We will begin to develop Aplysia as an aging model by studying the changes in D-Asp- and other agonist-activated ionic currents that modulate sensory neuron input in the tail withdrawal reflex, using single cell voltage clamping and recording of the intact reflex. Through this combination of production and basic research, we will improve the model system and extend its usefulness to other areas of research. RELEVANCE (See instructions): The California sea hare, Aplysia californica, is a large marine snail with a very simple nervous system. The National Resource for Aplysia raises and ships 25,000 to 30,00 of these animals per year to labs in the US and around the world for conducting basic research in neurobiology and behavior. This proposal would continue the production of these animals and expand their usefulness concerning studies of aging and Alzheimer's disease.