Histopathology

组织病理学

• 批准号: 8320336
• 负责人: PELAYO none CORREA
• 金额: $ 12.81万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8320336
• 关键词: 3-nitrotyrosine; Biopsy; Buffers; Caring; Colombia; DNA; Disease; Evaluation; Formalin; Freezing; Frozen Sections; Gastroscopes; Helicobacter pylori; Hematoxylin and Eosin Staining Method; Histology; Histopathology; Human; Human Resources; Ice; Infection; Laboratories; Leukocytes; Methylation; Microdissection; Microscope; Mucous Membrane; Paraffin; Paraffin Embedding; Procedures; Process; Research Personnel; Rodent; Silver Staining; Staining method; Stains; Stomach; Stomach Carcinoma; TdT-Mediated dUTP Nick End Labeling Assay; Tissues; Training; Zinc; experience; human NOS2A protein; tissue processing

Core A takes care of the tissue processing needs for all projects. It is directly supervised by Dr. Pelayo Correa and his assistant Dr. M. Blanca Piazuelo. In Nashville our personnel receive all biopsies from Colombia. Biopsies for histopathology evaluation are fixed immediately in buffered zinc-formalin, embedded in paraffin, sectioned and stained with hematoxylin eosin and modified Warthin- Starry silver stain. Biopsies for frozen sections are received in OCT and frozen immediately. The laboratory performs all immunohistochemical stains, including Ki67, TUNEL assays, inducible nitric oxide synthase, nitrotyrosine and a variety of white blood cell markers. Fully trained and experienced personnel are available for all procedures.

核心 A 负责所有项目的组织处理需求。它由 Pelayo 博士直接监督 科雷亚和他的助手 M. Blanca Piazuelo 博士。在纳什维尔，我们的人员接受来自以下组织的所有活检： 哥伦比亚。用于组织病理学评估的活组织检查立即固定在缓冲的福尔马林锌中，包埋 石蜡中，切片并用苏木精伊红和改良的 Warthin-Starry 银染色剂染色。活检 冷冻切片在 OCT 中收到并立即冷冻。实验室执行所有 免疫组织化学染色，包括 Ki67、TUNEL 检测、诱导型一氧化氮合酶、硝基酪氨酸 以及多种白细胞标记物。训练有素且经验丰富的人员可供所有人使用 程序。