Histopathology

组织病理学

• 批准号: 7749287
• 负责人: PELAYO none CORREA
• 金额: $ 13.13万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7749287
• 关键词: 3-nitrotyrosine; Biopsy; Buffers; Caring; Colombia; DNA; Disease; Evaluation; Formalin; Freezing; Frozen Sections; Gastroscopes; Helicobacter pylori; Hematoxylin and Eosin Staining Method; Histology; Histopathology; Human; Human Resources; Ice; Infection; Laboratories; Leukocytes; Methylation; Microdissection; Microscope; Mucous Membrane; Paraffin; Paraffin Embedding; Procedures; Process; Research Personnel; Rodent; Silver Staining; Staining method; Stains; Stomach; Stomach Carcinoma; TdT-Mediated dUTP Nick End Labeling Assay; Tissues; Training; Zinc; experience; human NOS2A protein; tissue processing

Core A takes care of the tissue processing needs for all projects. It is directly supervised by Dr. Pelayo Correa and his assistant Dr. M. Blanca Piazuelo. In Nashville our personnel receive all biopsies from Colombia. Biopsies for histopathology evaluation are fixed immediately in buffered zinc-formalin, embedded in paraffin, sectioned and stained with hematoxylin eosin and modified Warthin- Starry silver stain. Biopsies for frozen sections are received in OCT and frozen immediately. The laboratory performs all immunohistochemical stains, including Ki67, TUNEL assays, inducible nitric oxide synthase, nitrotyrosine and a variety of white blood cell markers. Fully trained and experienced personnel are available for all procedures.

核心A负责所有项目的组织处理需求。它由佩拉约博士直接监督 科雷亚和他的助手M·布兰卡·皮亚苏埃洛博士。在纳什维尔，我们的工作人员收到了来自 哥伦比亚。用于组织病理学评估的活检立即固定在缓冲锌-福尔马林中，包埋 石蜡切片，苏木精-伊红染色和改良Warthing-Starry银染。活组织检查 对于冻结的切片，在10月份收到并立即冻结。实验室进行了所有的 免疫组织化学染色，包括Ki67、TUNEL法、诱导型一氧化氮合酶、硝基酪氨酸 以及各种白血球标志物。所有人都可以获得经过充分培训和经验丰富的人员 程序。