GENE-TARGETTED SNP DISCOVERY IN RHESUS MACAQUES

恒河猴中基因靶向 SNP 的发现

• 批准号: 8357783
• 负责人: BETSY M FERGUSON
• 金额: $ 5.82万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357783
• 关键词: 3' Untranslated Regions; Chinese People; Data; Databases; Funding; Gene Expression Profile; Gene Frequency; Gene Targeting; Genes; Grant; Macaca mulatta; Messenger RNA; National Center for Research Resources; Population; Prefrontal Cortex; Primates; Principal Investigator; Reading; Reporting; Research; Research Infrastructure; Resources; Single Nucleotide Polymorphism; Source; Technology; United States National Institutes of Health; cost; next generation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project originally aimed to identify single nucleotide polymorphisms (SNPs) in 10,000 gene regions, by sequencing the 3' UTR of unrelated Chinese and Indian rhesus macaques. The compiled SNP data will be analyzed to identify SNPs and allele frequencies in both Indian and Chinese populations and to report the findings in the public databases dbSNP and MonkeySNP. In the past year we adjusted our SNP discovery strategy to include next generation sequencing technologies (Illumina short-read formats). We completed transcriptome sequencing using mRNA from the prefrontal cortex of six Indian and six Chinese rhesus macaques. Sanger re-sequencing was used to validate the candidate SNPs and to inform filtering limits to insure robust data. Over 1 million SNPs were detected in this study thus far.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目最初旨在通过对无关的中国和印度恒河猴的3' UTR进行测序，在10，000个基因区域中鉴定单核苷酸多态性（SNP）。将分析汇编的SNP数据，以确定印度和中国人群中的SNP和等位基因频率，并在公共数据库dbSNP和MonkeySNP中报告结果。在过去的一年中，我们调整了SNP发现策略，以包括下一代测序技术（Illumina短读格式）。 我们完成了转录组测序的mRNA从前额叶皮质的六个印度和六个中国恒河猴。使用桑格重新测序来验证候选SNP并告知过滤限制以确保稳健的数据。到目前为止，在这项研究中检测到超过100万个SNP。