SNP DISCOVERY AND LD ANALYSIS IN RHESUS MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目旨在鉴定300个恒河猴基因的单核苷酸多态性（SNPs），并利用这些SNPs来评估基因间的连锁不平衡（LD）程度。具体而言，将在24只无关的中国和印度恒河猴中分析与非人灵长类研究显著相关的300个基因。将对每个基因的三个不同区域（5 '、3'和一个中间区域）进行PCR扩增、测序和比较，以鉴定序列变体。将分析汇编的SNP数据，以确定印度和中国人群中的等位基因频率。SNP等位基因也将用于估计LD随物理距离衰减的速率。结果将在公共数据库dbSNP和MonkeySNP中报告。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to identify single nucleotide polymorphisms (SNPs) in 300 rhesus macaque genes and to use those SNPs to assess the extent of linkage disequilibrium (LD) across the genes. Specifically, 300 genes with significant relevance to non-human primate research will be analyzed in 24 unrelated Chinese and Indian rhesus macaques. Three distinct regions per gene (5', 3' and one intermediate region) will be PCR amplified, sequenced and compared to identify sequence variants. The compiled SNP data will be analyzed to identify allele frequencies in both Indian and Chinese populations. The SNP alleles will also be used to estimate the rate of LD decay with physical distance. The results will be reported in the public databases dbSNP and MonkeySNP.