GENES CONTROLLING PUBERTY ONSET

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 目的：研究控制青春期发育的基因。 说明： 了解大脑中青春期的机制很重要，就像一些疾病一样 行为障碍与青春期有关。这项研究的目的是 是为了研究Kispeptins在青春期发育中的作用。 进度： 在这项研究中，Kispeptin在青春期GnRH释放增加中的作用是 检查过了。结果表明，Kispeptin-54在茎中释放 微透析法测量的隆起度是搏动性的，平均为Kispeptin-54 电平和脉冲频率，而不是脉冲幅度，在发病时增加 青春期。此外，青春期Kispeptin-54释放的增加是独立的。 从青春期循环中卵巢雌激素的变化来看，随着青春期的增加 在卵巢切除的女性体内，也观察到Kispeptin-54的释放。最后， GPR54对KP敏感性的发育变化通过评估 GnRH对KP激动剂和拮抗剂KP-10和肽234的反应。 结果Kp-10和234肽对GnRH的反应率均为 青春期前和青春期组之间的差异，表明GPR54敏感性 没有经历发育变化。总的来说，这些结果是一致的 假设Kispeptin在青春期起作用。 这项研究使用了WNPRC动物服务和化验服务。 出版物： 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 促性腺激素释放激素神经元调控的新发现 非人灵长类动物。J.神经内分泌。2010年22：630-638。PMID：20456608，PMCID： PMC2908205。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To investigate genes controlling the timing of puberty. DESCRIPTION: Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. The aim of this study is to investigate the role of kisspeptins in the timing of puberty. PROGRESS: In this study the role of kisspeptin in the pubertal increase in GnRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile and mean kisspeptin-54 levels and pulse frequency, but not pulse amplitude, increased at the onset of puberty. Moreover, the pubertal increase in kisspeptin-54 release was independent from the pubertal changes in circulating ovarian estrogens, as the pubertal increase in kisspeptin-54 release was also observed in ovariectomized females. Finally, developmental changes in GPR54 sensitivity to KP were examined by assessing the GnRH response to a KP agonist and antagonist, KP-10 and peptide 234, respectively. Results that the rate of GnRH response to neither KP-10 nor peptide 234 was different between prepubertal and pubertal groups, indicate that GPR54 sensitivity does not undergo developmental changes. Collectively, these results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services. PUBLICATION: Terasawa, E., Kurian, J.R., Guerriero, K.A., Kenealy, B.P., Hutz, E.D., and Keen, K.L. Recent discoveries on the control of gonadotrophin-releasing hormone neuronesin nonhuman primates. J. Neuroendocrinol. 22:630-638, 2010. PMID: 20456608, PMCID: PMC2908205.