GENES CONTROLLING PUBERTY ONSET

控制青春期开始的基因

• 批准号: 8358199
• 负责人: Ei Terasawa-Grilley
• 金额: $ 11.57万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358199
• 关键词: Agonist; Animals; Behavioral; Biological Assay; Brain; Development; Disease; Estrogens; Female; Frequencies; Funding; Genes; Gonadotropin Hormone Releasing Hormone; Grant; KISS1R gene; Measures; Methods; Microdialysis; National Center for Research Resources; Ovarian; Peptides; Physiologic pulse; Play; Primates; Principal Investigator; Puberty; Research; Research Infrastructure; Resources; Role; Services; Source; Time; United States National Institutes of Health; Wisconsin; cost; kisspeptin; median eminence; nonhuman primate; prepuberty; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To investigate genes controlling the timing of puberty. DESCRIPTION: Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. The aim of this study is to investigate the role of kisspeptins in the timing of puberty. PROGRESS: In this study the role of kisspeptin in the pubertal increase in GnRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile and mean kisspeptin-54 levels and pulse frequency, but not pulse amplitude, increased at the onset of puberty. Moreover, the pubertal increase in kisspeptin-54 release was independent from the pubertal changes in circulating ovarian estrogens, as the pubertal increase in kisspeptin-54 release was also observed in ovariectomized females. Finally, developmental changes in GPR54 sensitivity to KP were examined by assessing the GnRH response to a KP agonist and antagonist, KP-10 and peptide 234, respectively. Results that the rate of GnRH response to neither KP-10 nor peptide 234 was different between prepubertal and pubertal groups, indicate that GPR54 sensitivity does not undergo developmental changes. Collectively, these results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services. PUBLICATION: Terasawa, E., Kurian, J.R., Guerriero, K.A., Kenealy, B.P., Hutz, E.D., and Keen, K.L. Recent discoveries on the control of gonadotrophin-releasing hormone neuronesin nonhuman primates. J. Neuroendocrinol. 22:630-638, 2010. PMID: 20456608, PMCID: PMC2908205.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 目的：探讨控制青春发育时间的基因。 产品说明： 了解大脑中青春期的机制很重要，因为一些疾病 和行为障碍与青春期有关。本研究的目的 是研究kisspeptins在青春期发育中的作用。 进展： 在这项研究中，kisspeptin在青春期GnRH释放增加中的作用是 考察结果表明，kisspeptin-54在茎中部释放， 微透析法测量的隆起是脉动的，平均kisspeptin-54 水平和脉冲频率，但不是脉冲幅度，增加了发病时， 青春期此外，青春期kisspeptin-54释放的增加是独立的， 随着青春期的增加，循环卵巢雌激素在青春期的变化 在卵巢切除的雌性中也观察到Kisspeptin-54的释放。最后， GPR 54对KP敏感性的发育变化通过评估 GnRH分别对KP激动剂和拮抗剂KP-10和肽234的反应。 结果表明，GnRH对KP-10和肽234均无反应， 青春期前和青春期组之间的差异，表明GPR 54敏感性 不会发生发育变化。总的来说，这些结果是一致的 kisspeptin在青春期起作用的假设。 本研究使用WNPRC Animal Services和Assay Services。 出版物： Terasawa，E.，Kurian，J.R.，Guerriero，K.A.，Kenealy，B.P.，Hutz，E. D.，和Keen，K.L. 促性腺激素释放激素神经元蛋白调控的新发现 非人类灵长类动物J. Neuroendocrinol. 22：630-638，2010. PMID：20456608，PMCID： PMC2908205。