GENE EXPRESSION IN 3D FOLLICLES
3D 卵泡中的基因表达
基本信息
- 批准号:8357851
- 负责人:
- 金额:$ 4.36万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-05-01 至 2012-04-30
- 项目状态:已结题
- 来源:
- 关键词:AntralBiological PreservationCancer SurvivorCell physiologyCellsComplexDevelopmentEncapsulatedFemaleFertilityFertilizationFundingGene ExpressionGrantHormonesHumanIntracytoplasmic Sperm InjectionsKnowledgeLifeMacacaMetaphaseMusNational Center for Research ResourcesOocytesOvulationPilot ProjectsPrimatesPrincipal InvestigatorProcessResearchResearch InfrastructureResourcesSomatic CellSourceStagingStimulusSystemTechnologyTranslationsUnited States National Institutes of HealthWomanbasecostembryonic cleavagefolliculogenesisin vivononhuman primateoffspringtheca cell
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
This is a pilot project of the Roadmap Consortium (U54 RR02437, T. Woodruff, PI) entitled "The Oncofertility Consortium: Fertility Preservation for Women." The process of folliculogenesis in vivo requires the coordinated development and maturation of the follicle wall and the oocyte enclosed within cumulus cells for eventual ovulation and fertilization. The ability to grow mouse follicles in a three-dimensional (3D) culture system yielding mature oocytes capable of fertilization and development to live offspring formed the basis of our current studies in nonhuman primates. We found that macaque follicles can survive, grow, form an antrum and secrete hormones when grown in encapsulated 3D culture. Despite exposure of macaque small antral follicles derived from encapsulated 3D culture to a maturation stimulus, most of the healthy oocytes remained immature. However, two oocytes matured to metaphase II, one fertilized via intracytoplasmic sperm injection and underwent early embryonic cleavage. Encapsulated 3D culture of macaque follicles to the small antral stage allows the functional gene expression of each follicular compartment (somatic cells, oocytes) to be analyzed separately and compared to follicles that develop to an equivalent stage/size in vivo. These studies will assess whether the encapsulated 3D system allows coordinated development of granulosa/theca cells and cumulus-oocyte complexes similar to that in vivo, and if not, will help define cellular functions that require further optimization in the 3D follicle culture system. This knowledge is critical for optimizing the 3D follicle culture technology for translation to human studies which will contribute to fertility preservation for female cancer survivors.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Mary B Zelinski其他文献
Mary B Zelinski的其他文献
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{{ truncateString('Mary B Zelinski', 18)}}的其他基金
Cryopreservation and Transplantation of Ovarian Cortical Tissue for Fertility Preservation
卵巢皮质组织的冷冻保存和移植以保存生育能力
- 批准号:
9920744 - 财政年份:2016
- 资助金额:
$ 4.36万 - 项目类别:
Cryopreservation and Transplantation of Ovarian Cortical Tissue for Fertility Preservation
卵巢皮质组织的冷冻保存和移植以保存生育能力
- 批准号:
9288194 - 财政年份:2016
- 资助金额:
$ 4.36万 - 项目类别:
PRE-CLINICAL TRIALS FOR FEMALE FERTILITY PRESERVATION
女性生育力保存的临床前试验
- 批准号:
8357745 - 财政年份:2011
- 资助金额:
$ 4.36万 - 项目类别:
IMPACT OF MATERNAL HIGH FAT DIET ON OFFSPRING OVARIAN FUNCTION
母亲高脂肪饮食对后代卵巢功能的影响
- 批准号:
8357852 - 财政年份:2011
- 资助金额:
$ 4.36万 - 项目类别:
ROLE OF STRESS IN PCOS: NEURONAL MECHANISMS
压力在多囊卵巢综合症中的作用:神经机制
- 批准号:
8357853 - 财政年份:2011
- 资助金额:
$ 4.36万 - 项目类别:
AMH AS PREDICTOR OF FOLLICLE FUNCTION DURING ENCAPSULATED 3D CULTURE IN MACAQUES
AMH 作为猕猴封装 3D 培养期间卵泡功能的预测因子
- 批准号:
8357774 - 财政年份:2011
- 资助金额:
$ 4.36万 - 项目类别:
OVARIAN TISSUE CRYOPRESERVATION IN NONHUMAN PRIMATES
非人类灵长类动物的卵巢组织冷冻保存
- 批准号:
8357823 - 财政年份:2011
- 资助金额:
$ 4.36万 - 项目类别:
AMH AS PREDICTOR OF FOLLICLE FUNCTION DURING ENCAPSULATED 3D CULTURE IN MACAQUES
AMH 作为猕猴封装 3D 培养期间卵泡功能的预测因子
- 批准号:
8173239 - 财政年份:2010
- 资助金额:
$ 4.36万 - 项目类别:
PRE-CLINICAL TRIALS FOR FEMALE FERTILITY PRESERVATION
女性生育力保存的临床前试验
- 批准号:
8173193 - 财政年份:2010
- 资助金额:
$ 4.36万 - 项目类别: