OVARIAN TISSUE CRYOPRESERVATION IN NONHUMAN PRIMATES
非人类灵长类动物的卵巢组织冷冻保存
基本信息
- 批准号:8357823
- 负责人:
- 金额:$ 5.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-05-01 至 2012-04-30
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAutologousBiocompatible MaterialsBiological PreservationCancer PatientCancer SurvivorClinicalCryopreservationEmbryonic DevelopmentEncapsulatedFemaleFertilityFreezingFundingGoalsGrantHumanLeadershipLifeMacacaMalignant NeoplasmsMethodsMorphologyNational Center for Research ResourcesOocytesOvarianOvarian TissuePatientsPhysiciansPolymersPrimatesPrincipal InvestigatorResearchResearch InfrastructureResourcesRiskSolutionsSourceTechnologyTimeTissuesTransplantationUnited States National Institutes of HealthUniversitiesWomancostimprovednonhuman primatenoveloffspringsteroid hormone
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
This project is part of a Roadmap Consortium (U54 RR02437, T. Woodruff, PI) entitled "The Oncofertility Consortium: Fertility Preservation for Women". The goal is to provide leadership in state-of-the-art cryopreservation technology to practitioners who are preserving human ovarian tissues under the aegis of the National Physicians Cooperative. The specific aim is to optimize ovarian cryopreservation methods in nonhuman primates for restoring fertility in female cancer survivors by comparing novel vitrification freeze-thaw methods to the currently utilized slow freeze method. Although ovarian transplantation after cryopreservation has yielded live human offspring, autologous transfer in many cancer patients carries the risk of re-seeding the patient with her own cancer. In these patients, encapsulated 3-dimensional (3D) culture of isolated secondary follicles post-thaw may be the best option for fertility preservation. Since slow freezing damages secondary follicles, optimization of ovarian tissue vitrification could bridge a technical gap for patients to utilize frozen ovarian tissue outside of transplantation. For the first time, macaque follicles isolated from vitrified tissue can survive, grow, form an antrum and produce steroid hormones, indicating functional preservation of cryopreserved secondary follicles. The addition of non-permeating polymers to the vitrification solution improved tissue morphology and follicle function in 3D culture. Current efforts are aimed at producing a mature oocyte derived from 3D follicle culture that can fertilize, undergo embryonic development and ultimately produce a live offspring. This project is facilitated by interactions with the Biomaterials Core at Northwestern University. Expected advances will be transferred to clinical efforts at Fertility Centers.
这个子项目是利用资源的许多研究子项目之一。
由NIH/NCRR资助的中心拨款提供。对子项目的主要支持
子项目的首席调查员可能是由其他来源提供的,
包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能
表示该子项目使用的中心基础设施的估计数量,
不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。
该项目是题为“肿瘤性生育联盟:为妇女保留生育力”的路线图联盟(U54 RR02437,T.Woodruff,PI)的一部分。其目标是为在国家医生合作社的支持下保存人类卵巢组织的从业者提供最先进的冷冻保存技术方面的领先地位。其具体目的是通过比较新的玻璃化冷冻-解冻方法和目前使用的慢速冷冻方法,优化非人类灵长类动物的卵巢冷冻保存方法,以恢复女性癌症幸存者的生育能力。尽管冷冻后的卵巢移植已经产生了活的人类后代,但在许多癌症患者中进行自体移植存在重新种植患者自己的癌症的风险。在这些患者中,解冻后分离的次级卵泡的包裹式三维(3D)培养可能是保留生育能力的最佳选择。由于缓慢冷冻会损害次级卵泡,优化卵巢组织玻璃化冷冻可以弥补患者在移植外利用冷冻卵巢组织的技术差距。从玻璃化组织中分离出的猕猴卵泡首次能够存活、生长、形成腔并产生类固醇激素,这表明冷冻保存的次级卵泡具有功能性。在3D培养中,在玻璃化溶液中加入非渗透聚合物改善了组织形态和卵泡功能。目前的努力旨在生产一种来自3D卵泡培养的成熟卵母细胞,这种卵母细胞可以受精,经历胚胎发育,并最终产生活的后代。该项目由与西北大学生物材料核心中心的互动推动。预期的进展将转移到生育中心的临床工作中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Mary B Zelinski其他文献
Mary B Zelinski的其他文献
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{{ truncateString('Mary B Zelinski', 18)}}的其他基金
Cryopreservation and Transplantation of Ovarian Cortical Tissue for Fertility Preservation
卵巢皮质组织的冷冻保存和移植以保存生育能力
- 批准号:
9920744 - 财政年份:2016
- 资助金额:
$ 5.82万 - 项目类别:
Cryopreservation and Transplantation of Ovarian Cortical Tissue for Fertility Preservation
卵巢皮质组织的冷冻保存和移植以保存生育能力
- 批准号:
9288194 - 财政年份:2016
- 资助金额:
$ 5.82万 - 项目类别:
PRE-CLINICAL TRIALS FOR FEMALE FERTILITY PRESERVATION
女性生育力保存的临床前试验
- 批准号:
8357745 - 财政年份:2011
- 资助金额:
$ 5.82万 - 项目类别:
IMPACT OF MATERNAL HIGH FAT DIET ON OFFSPRING OVARIAN FUNCTION
母亲高脂肪饮食对后代卵巢功能的影响
- 批准号:
8357852 - 财政年份:2011
- 资助金额:
$ 5.82万 - 项目类别:
ROLE OF STRESS IN PCOS: NEURONAL MECHANISMS
压力在多囊卵巢综合症中的作用:神经机制
- 批准号:
8357853 - 财政年份:2011
- 资助金额:
$ 5.82万 - 项目类别:
AMH AS PREDICTOR OF FOLLICLE FUNCTION DURING ENCAPSULATED 3D CULTURE IN MACAQUES
AMH 作为猕猴封装 3D 培养期间卵泡功能的预测因子
- 批准号:
8357774 - 财政年份:2011
- 资助金额:
$ 5.82万 - 项目类别:
AMH AS PREDICTOR OF FOLLICLE FUNCTION DURING ENCAPSULATED 3D CULTURE IN MACAQUES
AMH 作为猕猴封装 3D 培养期间卵泡功能的预测因子
- 批准号:
8173239 - 财政年份:2010
- 资助金额:
$ 5.82万 - 项目类别:
PRE-CLINICAL TRIALS FOR FEMALE FERTILITY PRESERVATION
女性生育力保存的临床前试验
- 批准号:
8173193 - 财政年份:2010
- 资助金额:
$ 5.82万 - 项目类别:
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