OVARIAN TISSUE CRYOPRESERVATION IN NONHUMAN PRIMATES

非人类灵长类动物的卵巢组织冷冻保存

• 批准号: 8357823
• 负责人: Mary B Zelinski
• 金额: $ 5.82万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357823
• 关键词: 3-Dimensional; Autologous; Biocompatible Materials; Biological Preservation; Cancer Patient; Cancer Survivor; Clinical; Cryopreservation; Embryonic Development; Encapsulated; Female; Fertility; Freezing; Funding; Goals; Grant; Human; Leadership; Life; Macaca; Malignant Neoplasms; Methods; Morphology; National Center for Research Resources; Oocytes; Ovarian; Ovarian Tissue; Patients; Physicians; Polymers; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Risk; Solutions; Source; Technology; Time; Tissues; Transplantation; United States National Institutes of Health; Universities; Woman; cost; improved; nonhuman primate; novel; offspring; steroid hormone

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project is part of a Roadmap Consortium (U54 RR02437, T. Woodruff, PI) entitled "The Oncofertility Consortium: Fertility Preservation for Women". The goal is to provide leadership in state-of-the-art cryopreservation technology to practitioners who are preserving human ovarian tissues under the aegis of the National Physicians Cooperative. The specific aim is to optimize ovarian cryopreservation methods in nonhuman primates for restoring fertility in female cancer survivors by comparing novel vitrification freeze-thaw methods to the currently utilized slow freeze method. Although ovarian transplantation after cryopreservation has yielded live human offspring, autologous transfer in many cancer patients carries the risk of re-seeding the patient with her own cancer. In these patients, encapsulated 3-dimensional (3D) culture of isolated secondary follicles post-thaw may be the best option for fertility preservation. Since slow freezing damages secondary follicles, optimization of ovarian tissue vitrification could bridge a technical gap for patients to utilize frozen ovarian tissue outside of transplantation. For the first time, macaque follicles isolated from vitrified tissue can survive, grow, form an antrum and produce steroid hormones, indicating functional preservation of cryopreserved secondary follicles. The addition of non-permeating polymers to the vitrification solution improved tissue morphology and follicle function in 3D culture. Current efforts are aimed at producing a mature oocyte derived from 3D follicle culture that can fertilize, undergo embryonic development and ultimately produce a live offspring. This project is facilitated by interactions with the Biomaterials Core at Northwestern University. Expected advances will be transferred to clinical efforts at Fertility Centers.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该项目是题为“肿瘤性生育联盟：为妇女保留生育力”的路线图联盟(U54 RR02437，T.Woodruff，PI)的一部分。其目标是为在国家医生合作社的支持下保存人类卵巢组织的从业者提供最先进的冷冻保存技术方面的领先地位。其具体目的是通过比较新的玻璃化冷冻-解冻方法和目前使用的慢速冷冻方法，优化非人类灵长类动物的卵巢冷冻保存方法，以恢复女性癌症幸存者的生育能力。尽管冷冻后的卵巢移植已经产生了活的人类后代，但在许多癌症患者中进行自体移植存在重新种植患者自己的癌症的风险。在这些患者中，解冻后分离的次级卵泡的包裹式三维(3D)培养可能是保留生育能力的最佳选择。由于缓慢冷冻会损害次级卵泡，优化卵巢组织玻璃化冷冻可以弥补患者在移植外利用冷冻卵巢组织的技术差距。从玻璃化组织中分离出的猕猴卵泡首次能够存活、生长、形成腔并产生类固醇激素，这表明冷冻保存的次级卵泡具有功能性。在3D培养中，在玻璃化溶液中加入非渗透聚合物改善了组织形态和卵泡功能。目前的努力旨在生产一种来自3D卵泡培养的成熟卵母细胞，这种卵母细胞可以受精，经历胚胎发育，并最终产生活的后代。该项目由与西北大学生物材料核心中心的互动推动。预期的进展将转移到生育中心的临床工作中。