IMPACT OF MATERNAL HIGH FAT DIET ON OFFSPRING OVARIAN FUNCTION

母亲高脂肪饮食对后代卵巢功能的影响

• 批准号: 8357852
• 负责人: Mary B Zelinski
• 金额: $ 3.63万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357852
• 关键词: Adolescent; Adult; Apoptosis; Caloric Restriction; Child; Controlled Study; Diabetes Mellitus; Diet; Environment; Ethics; Fatty acid glycerol esters; Female; Female Adolescents; Fetal Development; Fetus; Funding; Grant; Infertility; Inflammation; Insulin Resistance; Macaca; Maternal Health; Metabolic; Morphology; Mothers; National Center for Research Resources; Neonatal; Obesity; Oocytes; Ovarian; Ovary; Pregnancy; Primates; Principal Investigator; Proliferation Marker; Puberty; Reporting; Research; Research Infrastructure; Resources; Risk; Rodent; Sheep; Source; Specimen; Syndrome; United States National Institutes of Health; Woman; cost; diabetic; dietary restriction; early onset; feeding; obesity in children; offspring; reproductive; reproductive function

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A dramatic increase in childhood obesity and diabetes is currently evident. While mainly attributed to calorically dense diets and a lack of activity, another contributing factor is maternal health and diet during pregnancy and the early neonatal period. Both intrauterine and neonatal environments contribute to metabolic abnormalities in offspring of rodents and sheep. Children born to diabetic women have abnormal adiposity, are insulin resistant, and have increased risk of developing early onset obesity. While numerous reports detail detrimental effects on reproductive function in offspring of mothers fed calorie-restricted diets during pregnancy, the direct impact of maternal obesity on reproductive function of female offspring is rarely studied. Obese adolescent girls are at increased risk for developing polycystic ovarian syndrome, a common form of infertility. There are no controlled studies in women and their female children relating the mother's diet during pregnancy to the child's propensity for reproductive anomalies either prior to or at puberty. There are also no ovarian specimens to examine from children due to ethical considerations. We preserved ovaries from adult female macaques fed control, or high fat diets, as well as female fetuses (gestational day 130) and female juvenile macaques derived from mothers fed control or high fat diets during pregnancy. Ovarian morphology and immunocytochemical markers of proliferation, apoptosis, vascularity, inflammation and oocyte integrity are being analyzed. These studies will determine the impact of maternal high fat diet on ovarian function of offspring in primates, and whether effects can be observed during fetal development and/or prior to puberty.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 目前，儿童肥胖和糖尿病的急剧增加是显而易见的。 虽然主要归因于热量密集的饮食和缺乏活动，但另一个影响因素是孕产妇健康以及怀孕期间和新生儿早期的饮食。 子宫内和新生儿环境都会导致啮齿动物和绵羊后代的代谢异常。 糖尿病女性所生的孩子患有异常肥胖、胰岛素抵抗，并且患早发性肥胖的风险增加。 尽管许多报告详细说明了在怀孕期间摄入热量限制饮食的母亲对后代生殖功能的不利影响，但很少研究母亲肥胖对女性后代生殖功能的直接影响。 肥胖的少女患多囊卵巢综合症的风险增加，多囊卵巢综合症是一种常见的不孕症。 目前还没有针对妇女及其女儿的对照研究，将母亲怀孕期间的饮食与孩子在青春期之前或青春期时出现生殖异常的倾向联系起来。 出于伦理考虑，也没有儿童卵巢标本可供检查。 我们保存了喂养对照或高脂肪饮食的成年雌性猕猴的卵巢，以及怀孕期间喂养对照或高脂肪饮食的雌性胎儿（妊娠第 130 天）和雌性幼年猕猴的卵巢。 正在分析卵巢形态和增殖、凋亡、血管分布、炎症和卵母细胞完整性的免疫细胞化学标记物。 这些研究将确定母亲高脂肪饮食对灵长类动物后代卵巢功能的影响，以及是否可以在胎儿发育期间和/或青春期之前观察到影响。