IMPACT OF MATERNAL HIGH FAT DIET ON OFFSPRING OVARIAN FUNCTION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 儿童肥胖症和糖尿病的急剧增加目前是显而易见的。 虽然主要归因于高热量饮食和缺乏活动，但另一个促成因素是孕产妇健康和怀孕期间和新生儿早期的饮食。 子宫内和新生儿的环境都有助于啮齿动物和绵羊后代的代谢异常。 糖尿病妇女所生的孩子有异常的肥胖，胰岛素抵抗，并增加了早发性肥胖的风险。 虽然许多报告详细说明了母亲在怀孕期间喂食热量限制饮食对后代生殖功能的不利影响，但很少研究母亲肥胖对雌性后代生殖功能的直接影响。 肥胖的青春期女孩患多囊卵巢综合征的风险增加，这是一种常见的不孕症。 没有对妇女及其女童进行对照研究，将母亲怀孕期间的饮食与儿童在青春期之前或青春期出现生殖异常的倾向联系起来。 出于伦理方面的考虑，也没有儿童的卵巢标本可供检查。 我们保存了喂食对照或高脂饮食的成年雌性猕猴的卵巢，以及妊娠期间喂食对照或高脂饮食的雌性胎儿（妊娠第130天）和雌性幼龄猕猴的卵巢。 卵巢形态学和增殖、凋亡、血管分布、炎症和卵母细胞完整性的免疫细胞化学标志物正在进行分析。 这些研究将确定母体高脂肪饮食对灵长类动物后代卵巢功能的影响，以及是否可以在胎儿发育期间和/或青春期前观察到影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A dramatic increase in childhood obesity and diabetes is currently evident. While mainly attributed to calorically dense diets and a lack of activity, another contributing factor is maternal health and diet during pregnancy and the early neonatal period. Both intrauterine and neonatal environments contribute to metabolic abnormalities in offspring of rodents and sheep. Children born to diabetic women have abnormal adiposity, are insulin resistant, and have increased risk of developing early onset obesity. While numerous reports detail detrimental effects on reproductive function in offspring of mothers fed calorie-restricted diets during pregnancy, the direct impact of maternal obesity on reproductive function of female offspring is rarely studied. Obese adolescent girls are at increased risk for developing polycystic ovarian syndrome, a common form of infertility. There are no controlled studies in women and their female children relating the mother's diet during pregnancy to the child's propensity for reproductive anomalies either prior to or at puberty. There are also no ovarian specimens to examine from children due to ethical considerations. We preserved ovaries from adult female macaques fed control, or high fat diets, as well as female fetuses (gestational day 130) and female juvenile macaques derived from mothers fed control or high fat diets during pregnancy. Ovarian morphology and immunocytochemical markers of proliferation, apoptosis, vascularity, inflammation and oocyte integrity are being analyzed. These studies will determine the impact of maternal high fat diet on ovarian function of offspring in primates, and whether effects can be observed during fetal development and/or prior to puberty.