ASSESSMENT OF FORESKIN/PENILE SIV CHALLENGE IN MACAQUES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这些实验将为SIV包皮/阴茎猕猴攻击模型的开发提供重要的初步数据。 基于默克STEP试验的发现，其中接种包皮的男性更有可能成为HIV+，我们建议在未来的SIV疫苗中应利用包皮攻毒途径作为粘膜攻毒途径。优化包皮/阴茎攻击途径对于在SIV/猴模型中通过这一重要粘膜途径测试疫苗将是重要的。 这些实验利用与细菌杜克雷嗜血杆菌（软下疳的病原体）的共感染模型来评估影响SIV通过包皮感染的因素。 最近的实验是在诱导H. Ducreyi感染，H. Ducreyi通过两种不同的感染途径（皮内和皮下）给予猕猴的包皮区域。 目前正在对这些实验进行评估。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. These experiments will provide important preliminary data for the development of the SIV foreskin/penile macaque challenge model. Based on the finding from the Merck STEP trial, in which vaccinated men with a foreskin were more likely to become HIV+, we propose that the foreskin challenge route should be utilized as a mucosal challenge route in future SIV vaccines. Optimizing the foreskin/penile challenge route will be important to test vaccines via this important mucosal route in the SIV/monkey model. These experiments utilize a coinfection model with the bacteria Haemophilus Ducreyi (causative agent of chancroid) to evaluate the factors that influence SIV infection via the foreskin. The most recent experiment is one in which foreskin/penile SIV transmission is assessed following the induction of H. Ducreyi infection, with the H. Ducreyi being administered to the foreskin area of the macaques via two different infection routes (intradermal and subcutaneously). Assessment of these experiments is currently underway.