NEUTROPENIA FOLLOWING G-CSF ADMINISTRATION

G-CSF 给药后中性粒细胞减少症

• 批准号: 8358172
• 负责人: Xavier Alvarez-Hernandez
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358172
• 关键词: Antibodies; Antigens; Blood Circulation; CSF3 gene; Confocal Microscopy; Endothelium; Funding; Grant; Label; Lung; Myelogenous; National Center for Research Resources; Neutropenia; Nuclear; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; Splenic Tissue; Staining method; Stains; United States National Institutes of Health; caveolin 1; cost; histiocyte; monocyte; neutrophil

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Shortly following G-CSF administration there is a dramatic yet transient disappearance of neutrophils from the circulation. The transient loss of neutrophils from the circulation following G-CSF administration appeared to be due to an accumulation of neutrophils within the pulmonary and splenic tissues. Detailed confocal microscopy using a myeloid/histiocyte antigen clone Mac387 which labels neutrophils and monocytes, an endothelial marker anti-caveolin-1-Cy3 antibody, and a nuclear stain (To-Pro3) showed that the neutrophils were elongated, appeared activated, and were tightly adhered to the endothelium of the pulmonary vasculature. These results suggest that the transient neutropenia following G-CSF administration is associated with an accumulation of neutrophils within the pulmonary and splenic tissues.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 G-CSF给药后不久，循环中嗜中性粒细胞的急剧消失了。 G-CSF给药后循环中嗜中性粒细胞的瞬时丧失似乎是由于肺和脾组织中嗜中性粒细胞的积累。使用髓样/组织细胞抗原克隆MAC387，标记中性粒细胞和单核细胞，一种内皮标记抗蛋白抗蛋白-1-Cy3抗体，核染色（TO-PRO3）使用核细胞和单核细胞标记，详细的共聚焦显微镜检查（TO-PRO3）表明，中性粒细胞显着，并且在肺泡上呈现了无限的范围。这些结果表明，施用G-CSF后的瞬时中性粒细胞减少与肺和脾组织中嗜中性粒细胞的积累有关。