NEUTROPENIA FOLLOWING G-CSF ADMINISTRATION

G-CSF 给药后中性粒细胞减少症

• 批准号: 8358172
• 负责人: Xavier Alvarez-Hernandez
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358172
• 关键词: Antibodies; Antigens; Blood Circulation; CSF3 gene; Confocal Microscopy; Endothelium; Funding; Grant; Label; Lung; Myelogenous; National Center for Research Resources; Neutropenia; Nuclear; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; Splenic Tissue; Staining method; Stains; United States National Institutes of Health; caveolin 1; cost; histiocyte; monocyte; neutrophil

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Shortly following G-CSF administration there is a dramatic yet transient disappearance of neutrophils from the circulation. The transient loss of neutrophils from the circulation following G-CSF administration appeared to be due to an accumulation of neutrophils within the pulmonary and splenic tissues. Detailed confocal microscopy using a myeloid/histiocyte antigen clone Mac387 which labels neutrophils and monocytes, an endothelial marker anti-caveolin-1-Cy3 antibody, and a nuclear stain (To-Pro3) showed that the neutrophils were elongated, appeared activated, and were tightly adhered to the endothelium of the pulmonary vasculature. These results suggest that the transient neutropenia following G-CSF administration is associated with an accumulation of neutrophils within the pulmonary and splenic tissues.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 注射G-CSF后不久，中性粒细胞从循环中戏剧性地但短暂地消失。给予G-CSF后循环中的中性粒细胞一过性丢失似乎是由于中性粒细胞在肺和脾组织中的积聚。使用标记中性粒细胞和单核细胞的髓系/组织细胞抗原克隆Mac387、内皮标记物抗小窝蛋白-1-Cy3抗体和核染色(TO-Pro3)的详细共聚焦显微镜显示，中性粒细胞被拉长，似乎被激活，并紧密地附着在肺血管内皮细胞上。这些结果表明，给予G-CSF后的一过性中性粒细胞减少与中性粒细胞在肺和脾组织中的聚集有关。