Protein Core

将需要对符合以下条件的流感和乙肝抗原进行抗原特异性抗体定量 疫苗成分，以及非疫苗流感抗原，以确定交叉反应。测量 标准化的乙肝疫苗抗原将通过医疗服务提供商。然而，一个细心和 彻底定义流感抗原是这项提议的核心。核心将不得不准确地量化 血清抗体效价针对：i)流感疫苗成分抗原，ii)流感非疫苗成分抗原， 以及iii)来自不同流感毒株的血凝素。该核心将通过四个目标支持U19计划： 目的1：抗原特异性抗体和抗原特异性B细胞的定量检测。核心将会 用高保真的血清抗体检测流感抗原。这将支持项目1、2、3、4、 和5. 目的2：抗流感中和抗体的鉴定。核心将得分中和反- 流感血清抗体水平，并评价其交叉反应性。这将支持项目1、2、3、4和5。 目的3：测定血清细胞因子和趋化因子水平。核心将测量以下水平 血清和培养上清液中的多种细胞因子和趋化因子。这将支持项目1、2、3、4和 5. 目的4：检测抗自身抗原抗体。核心将进行蛋白质组范围的调查 项目3的自身抗体。这将支持项目4。 目的5：新型生物标志物检测试剂的研制。核心将开发新的蛋白质和 以抗体为基础的生物标记物，用于验证在所有项目区取得的发现。这将支持 项目1、2、3、4和5。

Antigen-specific antibody quantification will be required for Influenza and Hepatitis B antigens that are vaccine components, as well as non-vaccine Influenza antigens to define cross-reactivity. Measurements for standardized hepatitis B vaccine antigens will be through medical service providers. However, a careful and thorough definition of influenza antigens is central to this proposal. The core will have to accurately quantify serum antibody titers against: i) Flu vaccine component antigens, ii) Flu non-vaccine component antigens, and iii) hemagglutinins from different Flu strains. This core will support the U19 program through four aims: Aim 1: Quantification of antigen-specific antibodies and antigen-specific B cells. The core will measure with high fidelity serum antibodies against Influenza antigens. This will support Project 1, 2, 3, 4, and 5. Aim 2: Characterization of anti-Influenza neutralizing antibodies. The core will score neutralizing anti- Influenza serum antibody levels and appraise their cross-reactivity. This will support Project 1, 2, 3, 4, and 5. Aim 3: Measurement of serum cytokine and chemokine levels. The core will measure the levels of multiple cytokines and chemokines in sera and culture supernatants. This will support Project 1,2,3, 4, and 5. Aim 4: Detection of antibodies against self antigens. The core will perform proteome-wide surveys of auto-antibodies for Project 3. This will support Project 4. Aim 5: Development of novel biomarker detection reagents. The core will develop novel protein and antibody-based biomarkers for validation of discoveries made through all the Project areas. This will support Project 1, 2, 3,4, and 5.