Protein Core

对于流感和乙型肝炎抗原，需要进行抗原特异性抗体定量。 疫苗成分以及非疫苗流感抗原来定义交叉反应性。测量值 标准化乙型肝炎疫苗抗原将通过医疗服务提供者提供。然而，细心且 流感抗原的彻底定义是该提案的核心。核心必须准确量化 针对以下各项的血清抗体滴度：i) 流感疫苗成分抗原，ii) 流感非疫苗成分抗原， iii) 来自不同流感病毒株的血凝素。该核心将通过四个目标支持 U19 计划： 目标 1：抗原特异性抗体和抗原特异性 B 细胞的定量。核心将 使用针对流感抗原的高保真血清抗体进行测量。这将支持项目 1、2、3、4、 和 5. 目标 2：抗流感中和抗体的表征。核心将得分中和反 流感血清抗体水平并评估其交叉反应性。这将支持项目 1、2、3、4 和 5。 目标 3：测量血清细胞因子和趋化因子水平。核心将测量以下水平 血清和培养上清液中存在多种细胞因子和趋化因子。这将支持项目 1、2、3、4 和 5. 目标 4：检测针对自身抗原的抗体。核心将进行全蛋白质组调查 项目 3 的自身抗体。这将支持项目 4。 目标 5：开发新型生物标志物检测试剂。核心将开发新的蛋白质和 基于抗体的生物标志物，用于验证所有项目领域的发现。这将支持 项目 1、2、3、4 和 5。

Antigen-specific antibody quantification will be required for Influenza and Hepatitis B antigens that are vaccine components, as well as non-vaccine Influenza antigens to define cross-reactivity. Measurements for standardized hepatitis B vaccine antigens will be through medical service providers. However, a careful and thorough definition of influenza antigens is central to this proposal. The core will have to accurately quantify serum antibody titers against: i) Flu vaccine component antigens, ii) Flu non-vaccine component antigens, and iii) hemagglutinins from different Flu strains. This core will support the U19 program through four aims: Aim 1: Quantification of antigen-specific antibodies and antigen-specific B cells. The core will measure with high fidelity serum antibodies against Influenza antigens. This will support Project 1, 2, 3, 4, and 5. Aim 2: Characterization of anti-Influenza neutralizing antibodies. The core will score neutralizing anti- Influenza serum antibody levels and appraise their cross-reactivity. This will support Project 1, 2, 3, 4, and 5. Aim 3: Measurement of serum cytokine and chemokine levels. The core will measure the levels of multiple cytokines and chemokines in sera and culture supernatants. This will support Project 1,2,3, 4, and 5. Aim 4: Detection of antibodies against self antigens. The core will perform proteome-wide surveys of auto-antibodies for Project 3. This will support Project 4. Aim 5: Development of novel biomarker detection reagents. The core will develop novel protein and antibody-based biomarkers for validation of discoveries made through all the Project areas. This will support Project 1, 2, 3,4, and 5.